Name: aortic ring assay
Uploaded: 2009-11-24T15:33:00
Description: Watch this Scientific Journal Video about Aortic Ring Assay at JoVE.com

前一天的实验： <ol><li>允许BME（生长因子减少，Cultrex，Trevigen）解冻在冰上或4 ° C，一旦解冻保持在冰上，以防止重新巩固。 </li></ol>日实验： <ol><li>小鼠（6-7周龄）是麻醉和流血。 </li><li>到冷无菌PBS填充培养皿中取出胸主动脉。 </li><li>从周围的脂肪组织进行机械清洗。注意，避免在任何时间干燥主动脉。 </li><li>使用手术刀片，片主动脉平均为1毫米的戒指。必须是统一的，...

<ol>
	<li>Goodwin, A. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=In+vitro+assays+of+angiogenesis+for+assessment+of+angiogenic+and+anti-angiogenic+agents.">In vitro assays of angiogenesis for assessment of angiogenic and anti-angiogenic agents.</a> Microvascular Research. 74, 172-183 (2007).</li><li>Masson, V., Devy, L., Grignet-Debrus, C., Bernt, S., Bajou, K., Blacher, S., Roland, G., Chang, Y., Fong, T., Carmeliet, P., Foidart, J. M., Noel, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Mouse+aortic+ring+assay:+a+new+approach+of+the+molecular+genetics+of+angiogenesis.">Mouse aortic ring assay: a new approach of the molecular genetics of angiogenesis.</a> Biol. Proced. Online. 4, 24-31 (2002).</li></ol>

主动脉环实验的方式，以评估血管生成以及抗血管生成的因素是一个有益的工具。主动脉环招聘平滑肌细胞和周细胞生长的关联与内皮细胞管，这意味着它们在体内neovessels解剖学类似的船只。此外，小鼠主动脉环实验，认为对这一物种的基因工具繁多的优势。然而，有几个缺点主动脉环检测。首先， 在体内的血管生长发生微血管和大血管，如主动脉。其次，在处理环不一致和周围?...

aortic ring assay

血管生成，从先前存在的血管血管发芽与自然和病理过程。各种血管生成的实验涉及个别内皮细胞在培养条件（1）的研究。主动脉环实验是一种血管生成模型，器官培养的基础上。在这个实验中，血管生成血管生长的主动脉（从（2）修改）段。简言之，小鼠胸主动脉切除，脂肪层和外膜被删除，并准备戒指约1毫米长。然后嵌入在一个小的坚实的圆顶地下室矩阵提取物（BME）的48孔板个别水井内投，个人环。血管生成因子和血管生成抑制剂，可直接添加到戒指，和主动脉环和其他类型的细胞混合共培养可以旁分泌的血管生成影响的研究工作。发芽立体显微镜下观察期6-12天检查。由于大的变化，在主动脉段，实验的违规行为造成6 plicates，强烈建议。 Neovessel生长监测整个实验过程，并用相差显微镜成像，以及相关的血管生成和抗血管生成因子，细胞死亡标记和亚硝酸盐的测量收集上清。

Watch this Scientific Journal Video about Aortic Ring Assay at JoVE.com

Aortic Ring Assay

血管生成，从预先存在的血管的血管的萌芽，是与自然和病理过程中。在这里，我们展示了一个主动脉环实验允许的血管生成potentiators和抑制剂可直接添加到文化的主动脉环。发芽和neovessel产物，可以通过检查为期6-12天的主动脉环确定。

主动脉环含量

Angiogenesis, the sprouting of blood vessels from preexisting vasculature is associated with both natural and pathological processes. Various angiogenesis ...

Research

JoVE Journal

Biology

Creation of Murine Experimental Abdominal Aortic Aneurysms with Elastase 

创建小鼠实验与弹性蛋白酶的腹主动脉瘤

Transient intraluminal infusion of porcine pancreatic elastase into the infrarenal segment of the abdominal aorta is the most widely used animal model of ...

科研

生物学

Transverse Aortic Constriction in Mice

在小鼠体内的横向主动脉缩窄

Transverse aortic constriction (TAC) in the mouse is a commonly used experimental model for pressure overload-induced cardiac hypertrophy and heart ...

Intracellular Refolding Assay

细胞内复性检测

This protocol describes a method to measure the enzymatic activity of molecular chaperones in a cell-based system and the possible effects of compounds ...

Na&iuml;ve Adult Stem Cells Isolation from Primary Human Fibroblast Cultures

NaÃƒÆ’Ã‚Â¯ve Adult Stem Cells Isolation from Primary Human Fibroblast Cultures

天真成人干细胞的分离从初级人类成纤维细胞培养

Over the last decade, several adult stem cell  populations have been identified in human skin 1-4.  The isolation of multipotent adult dermal precursors ...

Super-resolution Imaging of the Cytokinetic Z Ring in Live Bacteria Using Fast 3D-Structured Illumination Microscopy (f3D-SIM)

Super-resolution Imaging of the Cytokinetic Z Ring in Live Bacteria Using Fast 3D-Structured Illumination Microscopy f3D-SIM

在Cytokineticž圈的活细菌超分辨率成像使用快速三维结构照明显微镜（F3D-SIM卡）

Imaging of biological samples using fluorescence microscopy has advanced substantially with new technologies to overcome the resolution barrier of the ...

Isolation and Primary Culture of Mouse Aortic Endothelial Cells

隔离和鼠标主动脉内皮细胞原代培养

The vascular endothelium is essential to normal vascular homeostasis. Its dysfunction participates in various cardiovascular disorders. The mouse is an ...

Murine Aortic Crush Injury: An Efficient In Vivo Model of Smooth Muscle Cell Proliferation and Endothelial Function

Murine Aortic Crush Injury: An Efficient In Vivo Model of Smooth Muscle Cell Proliferation and Endothelial Function

小鼠主动脉夹层损伤：高效<em&gt;体内</em&gt;平滑肌细胞增殖和内皮功能模型

Arterial reconstruction, whether angioplasty or bypass surgery, involves iatrogenic trauma causing endothelial disruption and vascular smooth muscle cell ...

Differentiation Capacity of Human Aortic Perivascular Adipose Progenitor Cells

人主动脉周脂肪祖细胞的分化能力

Adipose tissue is a rich source of multi-potent mesenchymal stem cells (MSC) capable of differentiating into osteogenic, adipogenic, and chondrogenic ...

Isolation and Culture of Primary Aortic Endothelial Cells from Miniature Pigs

微型猪原主主动脉内皮细胞的隔离与培养

Xenotransplantation is a promising way to resolve the shortage of human organs for patients with end-stage organ failure, and the pig is considered as a ...

Isolation of Mouse Interstitial Valve Cells to Study the Calcification of the Aortic Valve In Vitro

Isolation of Mouse Interstitial Valve Cells to Study the Calcification of the Aortic Valve In Vitro

小鼠间质瓣细胞分离研究主动脉瓣体外钙化

The calcification of aortic valve cells is the hallmark of aortic stenosis and is associated with valve cusp fibrosis. Valve interstitial cells (VICs) ...