Name: assembly, tuning and use of an apertureless near field infrared microscope for protein imaging
Uploaded: 2009-11-25T03:33:00
Description: Watch this Scientific Journal Video about Assembly, Tuning and Use of an Apertureless Near Field Infrared Microscope for Protein Imaging at JoVE.com

我们非常感谢美国国家科学基金会，NSERC，美国国立卫生研究院和ONR。

背景： Apertureless探头近场红外显微镜提供高空间分辨率的成像。它是一个相对较新的技术，其中一个事件的红外光束被锋利的原子力显微镜（AFM）在接近样品的悬臂共振频率振荡提示分散。红外探测器收集的散射光，并在这个共振频率及其谐波解调。这样一来，其余样品表面上的聚焦激光束事件的背景分散可以降低一个远远超出了光的衍射极限的空间分辨率可以达到获得与纳...

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assembly, tuning and use of an apertureless near field infrared microscope for protein imaging

本文旨在指导读者在超越衍射极限的成像的红外线近场显微镜的装配和操作。 apertureless近场显微镜是一种光散射式仪器，可提供大约20 nm分辨率的红外光谱。一个组件的完整列表和使用一步一步的协议提供。大会和仪器调谐常见的错误进行了讨论。一个有代表性的数据集，显示了二级结构的淀粉样原纤维。

Watch this Scientific Journal Video about Assembly, Tuning and Use of an Apertureless Near Field Infrared Microscope for Protein Imaging at JoVE.com

Assembly, Tuning and Use of an Apertureless Near Field Infrared Microscope for Protein Imaging

描述成像蛋白质总量的大会，是一个近场红外显微镜。

大会，调整和使用蛋白成像Apertureless近场红外显微镜

This paper aims to instruct the reader in the assembly and operation of an infrared near-field microscope for imaging beyond the diffraction limit.  The ...

Research

JoVE Journal

Biology

Proper Care and Cleaning of the Microscope

适当的照顾和清洁的显微镜

Keeping the microscope optics clean is important for high-quality imaging. Dust, fingerprints, excess immersion oil, or mounting medium on or in a ...

科研

生物学

Major Components of the Light Microscope

光镜下的主要组成部分

The light microscope is a basic tool for the cell biologist, who should have a thorough understanding of how it works, how it should be aligned for ...

Exploring Cognitive Functions in Babies, Children &amp; Adults with Near Infrared Spectroscopy

Exploring Cognitive Functions in Babies, Children & Adults with Near Infrared Spectroscopy

近红外光谱技术在婴儿，儿童及成人的认知功能探索

An explosion of functional Near Infrared Spectroscopy (fNIRS) studies investigating cortical activation in relation to higher cognitive processes, such as ...

Assembly, Loading, and Alignment of an Analytical Ultracentrifuge Sample Cell

大会，加载和分析超速离心机样品池对齐

The analytical ultracentrifuge (AUC) is a powerful biophysical tool that allows us to record macromolecular sedimentation profiles during high speed ...

Using the GELFREE 8100 Fractionation System for Molecular Weight-Based Fractionation with Liquid Phase Recovery

液相恢复GELFREE 8100分馏系统使用基于分子量分馏

The GELFREE 8100 Fractionation System is a novel protein fractionation system designed to maximize protein recovery during molecular weight based ...

Imaging C. elegans Embryos using an Epifluorescent Microscope and Open Source Software

Imaging C. elegans Embryos using an Epifluorescent Microscope and Open Source Software

成像 C。线虫胚胎使用啶显微镜和开放源码软件

Cellular processes, such as chromosome assembly, segregation and cytokinesis,are inherently dynamic. Time-lapse imaging of living cells, using ...

Automated Hydrophobic Interaction Chromatography Column Selection for Use in Protein Purification

自动疏水层析蛋白质纯化用柱选择

In contrast to other chromatographic methods for purifying proteins (e.g. gel filtration, affinity, and ion exchange), hydrophobic interaction ...

Method for the Assessment of Effects of a Range of Wavelengths and Intensities of Red/near-infrared Light Therapy on Oxidative Stress In Vitro

Method for the Assessment of Effects of a Range of Wavelengths and Intensities of Red/near-infrared Light Therapy on Oxidative Stress In Vitro

对于波长和红/近红外光治疗对氧化应激强度的范围影响的评估方法<em&gt;在体外</em

Red/near-infrared light therapy (R/NIR-LT), delivered by laser or light emitting diode (LED), improves functional and morphological outcomes in a range of ...

A Quantitative Dot Blot Assay for AAV Titration and Its Use for Functional Assessment of the Adeno-associated Virus Assembly-activating Proteins

AAV 滴定定量斑点印迹法及其在腺相关病毒组装活化蛋白功能评价中的应用

While adeno-associated virus (AAV) is widely accepted as an attractive vector for gene therapy, it also serves as a model virus for understanding virus ...

Detecting and Characterizing Protein Self-Assembly In Vivo by Flow Cytometry

通过流动细胞测定检测和描述 Vivo 中的蛋白质自组装

Protein self-assembly governs protein function and compartmentalizes cellular processes in space and time. Current methods to study it suffer from ...