Name: assembly, tuning and use of an apertureless near field infrared microscope for protein imaging
Uploaded: 2009-11-25T03:33:00
Description: Watch this Scientific Journal Video about Assembly, Tuning and Use of an Apertureless Near Field Infrared Microscope for Protein Imaging at JoVE.com

Noi riconosciamo con gratitudine NSF, NSERC, NIH, e ONR.

Di fondo: Apertureless sonda di campo vicino microscopia IR offre immagini ad alta risoluzione spaziale. Si tratta di una tecnica relativamente nuova, in cui è diffusa un fascio incidente a infrarossi da un forte microscopia a forza atomica (AFM) punta oscilla alla frequenza di risonanza del cantilever vicino al campione. Un rivelatore IR raccoglie la luce diffusa ed è demodulato a questa frequenza di risonanza o sue armoniche. In questo modo, la dispersione sfondo della vicenda fascio laser...

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assembly, tuning and use of an apertureless near field infrared microscope for protein imaging

Questo lavoro si propone di istruire il lettore nel montaggio e funzionamento di un vicino infrarosso microscopio a campo per l&#39;imaging oltre il limite di diffrazione. Il apertureless microscopio a campo vicino è una luce scattering-tipo di strumento che fornisce gli spettri infrarossi a circa 20 nm risoluzione. Un elenco completo dei componenti e uno step-by-step del protocollo per l&#39;uso è fornito. Gli errori più comuni in assemblea e messa a punto dello strumento sono discussi. Un insieme rappresentativo di dati che mostra la struttura secondaria di fibrille amiloidi è presentato.

Watch this Scientific Journal Video about Assembly, Tuning and Use of an Apertureless Near Field Infrared Microscope for Protein Imaging at JoVE.com

Assembly, Tuning and Use of an Apertureless Near Field Infrared Microscope for Protein Imaging

L&#39;assemblaggio di un microscopio a raggi infrarossi per nearfield aggregati proteici di imaging è descritto.

Assemblea, Tuning e utilizzo di un microscopio Apertureless Near Field Infrared for Imaging Proteine

This paper aims to instruct the reader in the assembly and operation of an infrared near-field microscope for imaging beyond the diffraction limit.  The ...

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