Name: assembly, tuning and use of an apertureless near field infrared microscope for protein imaging
Uploaded: 2009-11-25T03:33:00
Description: Watch this Scientific Journal Video about Assembly, Tuning and Use of an Apertureless Near Field Infrared Microscope for Protein Imaging at JoVE.com

우리는 기꺼이 NSF, NSERC, NIH, 그리고 ONR을 인정합니다.

배경 : Apertureless 프로브 니어 필드 적외선 현미경은 높은 공간 해상도의 영상을 제공합니다. 그것은 사건 적외선 광선이 가까운 샘플로 캔틸레버의 공진 주파수로 진동 날카로운 원자 힘 현미경 (AFM) 팁에 의해 흩어져있는 비교적 새로운 기술입니다. IR 탐지기가 흩어져 빛을를 수집하고이 공진 주파수 또는 그 고조파에 복조된입니다. 이러한 방법으로 샘플 표면의 나머지 부?...

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assembly, tuning and use of an apertureless near field infrared microscope for protein imaging

본 논문은 회절 한계 이상의 이미징을위한 적외선 니어 필드 현미경의 조립 및 운영에 독자를 지시하는 것을 목표로하고있다. apertureless 니어 필드 현미경 써카 20 nm의 해상도에서 적외선 스펙트럼을 제공하는 광 산란 형태의 악기이다. 구성 요소와 사용하기 위해 단계별로 프로토콜의 전체 목록이 제공됩니다. 조립 및 악기 튜닝에 일반 오류가 설명합니다. 아밀로이드 근모의 차 구조를 보여주는 대표적인 데이터 집합이 제공됩니다.

Watch this Scientific Journal Video about Assembly, Tuning and Use of an Apertureless Near Field Infrared Microscope for Protein Imaging at JoVE.com

Assembly, Tuning and Use of an Apertureless Near Field Infrared Microscope for Protein Imaging

이미징 단백질 집계에 nearfield 적외선 현미경의 어셈블리가 설명되어 있습니다.

조립, 조정 및 단백질 이미징을위한 Apertureless 니어 필드 적외선 분광 현미경의 사용

This paper aims to instruct the reader in the assembly and operation of an infrared near-field microscope for imaging beyond the diffraction limit.  The ...

Research

JoVE Journal

Biology

Proper Care and Cleaning of the Microscope

현미경의 올바른 관리 및 청소

Keeping the microscope optics clean is important for high-quality imaging. Dust, fingerprints, excess immersion oil, or mounting medium on or in a ...

연구

생물학

Major Components of the Light Microscope

라이트 현미경의 주요 구성 요소

The light microscope is a basic tool for the cell biologist, who should have a thorough understanding of how it works, how it should be aligned for ...

Exploring Cognitive Functions in Babies, Children &amp; Adults with Near Infrared Spectroscopy

Exploring Cognitive Functions in Babies, Children & Adults with Near Infrared Spectroscopy

가까운 적외선 분광학과 함께 아기, 어린이 및 성인의인지 기능을 탐색

An explosion of functional Near Infrared Spectroscopy (fNIRS) studies investigating cortical activation in relation to higher cognitive processes, such as ...

Assembly, Loading, and Alignment of an Analytical Ultracentrifuge Sample Cell

조립, 로딩 및 분석 초원 심 분리기 샘플 셀 정렬

The analytical ultracentrifuge (AUC) is a powerful biophysical tool that allows us to record macromolecular sedimentation profiles during high speed ...

Using the GELFREE 8100 Fractionation System for Molecular Weight-Based Fractionation with Liquid Phase Recovery

액상 복구와 분자량 기반 분류에 대한 GELFREE 8100 분획화 시스템을 사용하여

The GELFREE 8100 Fractionation System is a novel protein fractionation system designed to maximize protein recovery during molecular weight based ...

Imaging C. elegans Embryos using an Epifluorescent Microscope and Open Source Software

Imaging C. elegans Embryos using an Epifluorescent Microscope and Open Source Software

이미징 C. 엘레간스</emEpifluorescent 현미경과 오픈 소스 소프트웨어를 사용> 태아

Cellular processes, such as chromosome assembly, segregation and cytokinesis,are inherently dynamic. Time-lapse imaging of living cells, using ...

Automated Hydrophobic Interaction Chromatography Column Selection for Use in Protein Purification

단백질 정제에서 사용하기 위해 자동화된 소수성 상호 작용 크로마 토그래피 칼럼 선정

In contrast to other chromatographic methods for purifying proteins (e.g. gel filtration, affinity, and ion exchange), hydrophobic interaction ...

Method for the Assessment of Effects of a Range of Wavelengths and Intensities of Red/near-infrared Light Therapy on Oxidative Stress In Vitro

Method for the Assessment of Effects of a Range of Wavelengths and Intensities of Red/near-infrared Light Therapy on Oxidative Stress In Vitro

파장과 산화 스트레스에 레드 / 근적외선 치료의 강렬의 범위의 효과의 평가를위한 방법<em&gt; 체외</em

Red/near-infrared light therapy (R/NIR-LT), delivered by laser or light emitting diode (LED), improves functional and morphological outcomes in a range of ...

A Quantitative Dot Blot Assay for AAV Titration and Its Use for Functional Assessment of the Adeno-associated Virus Assembly-activating Proteins

AAV 적정 및 Adeno 관련 바이러스 어셈블리 활성화의 기능 평가 대 한 그것의 사용에 대 한 양적 점 오 점 분석 결과 단백질

While adeno-associated virus (AAV) is widely accepted as an attractive vector for gene therapy, it also serves as a model virus for understanding virus ...

Detecting and Characterizing Protein Self-Assembly In Vivo by Flow Cytometry

유세포분석으로 생체내 단백질 자가 조립 검출 및 특성화

Protein self-assembly governs protein function and compartmentalizes cellular processes in space and time. Current methods to study it suffer from ...