Name: assembly, tuning and use of an apertureless near field infrared microscope for protein imaging
Uploaded: 2009-11-25T03:33:00
Description: Watch this Scientific Journal Video about Assembly, Tuning and Use of an Apertureless Near Field Infrared Microscope for Protein Imaging at JoVE.com

Agradecemos NSF, NSERC, NIH, y ONR.

Antecedentes: Apertureless sonda cerca de la microscopía de campo IR ofrece imágenes de alta resolución espacial. Es una técnica relativamente nueva en la que se dispersa un haz incidente de infrarrojos por un fuerte microscopía de fuerza atómica (AFM) de punta que oscila a la frecuencia de resonancia del cantilever cerca de la muestra. Un detector de infrarrojos recoge la luz dispersada y se demodula en esta frecuencia de resonancia o sus armónicos. De esta manera, la dispersión de fo...

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assembly, tuning and use of an apertureless near field infrared microscope for protein imaging

Este documento tiene como objetivo instruir al lector en el montaje y operación de un infrarrojo cercano microscopio de campo para obtener imágenes más allá del límite de difracción. El apertureless cerca de microscopio de campo es una dispersión de luz, tipo de instrumento que proporciona espectros infrarrojos a una resolución de alrededor de 20 nm. Una lista completa de componentes y un protocolo de paso a paso para su uso se proporciona. Errores comunes en el montaje y ajuste de instrumentos se discuten. Un conjunto de datos representativo que muestra la estructura secundaria de un amiloide fibrilar se presenta.

Watch this Scientific Journal Video about Assembly, Tuning and Use of an Apertureless Near Field Infrared Microscope for Protein Imaging at JoVE.com

Assembly, Tuning and Use of an Apertureless Near Field Infrared Microscope for Protein Imaging

El montaje de un microscopio de campo cercano infrarrojo de agregados de proteínas de imagen se describe.

Montaje, ajuste y uso de un microscopio de campo Apertureless de Infrarrojo Cercano para la imagen de proteínas

This paper aims to instruct the reader in the assembly and operation of an infrared near-field microscope for imaging beyond the diffraction limit.  The ...

Research

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Biology

Proper Care and Cleaning of the Microscope

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Automated Hydrophobic Interaction Chromatography Column Selection for Use in Protein Purification

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