Name: assembly, tuning and use of an apertureless near field infrared microscope for protein imaging
Uploaded: 2009-11-25T03:33:00
Description: Watch this Scientific Journal Video about Assembly, Tuning and Use of an Apertureless Near Field Infrared Microscope for Protein Imaging at JoVE.com

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Bakgrund: Apertureless sond närområdet IR-mikroskopi ger hög rumslig upplösning. Det är en relativt ny teknik där en incident infraröd stråle är utspridd av en skarp atomkraftsmikroskopi (AFM) spets oscillerande vid resonansfrekvens av fribärande nära provet. En IR-detektor samlar spritt ljus och är demodulerade vid denna resonansfrekvens eller dess övertoner. På detta sätt kan bakgrunden skingra den fokuserade laserstrålen incident på resten av provets yta minskas och en rums...

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assembly, tuning and use of an apertureless near field infrared microscope for protein imaging

Detta dokument syftar till att instruera läsaren i montering och drift av en infraröd närområdet mikroskop för avbildning bortom diffraktionsgränsen. Den apertureless närområdet mikroskop är en ljusspridning typ instrument som ger infraröda spektra på ca 20 nm upplösning. En fullständig lista över komponenter och en steg-för-steg-protokoll för användningen. Vanliga fel i montering och instrument tuning diskuteras. En representant datauppsättning som visar den sekundära strukturen av en amyloid ROTHÅR presenteras.

Watch this Scientific Journal Video about Assembly, Tuning and Use of an Apertureless Near Field Infrared Microscope for Protein Imaging at JoVE.com

Assembly, Tuning and Use of an Apertureless Near Field Infrared Microscope for Protein Imaging

Montering av en nearfield infraröd mikroskop för avbildning protein aggregat beskrivs.

Montering, Tuning och användning av en Apertureless Near Field Infraröd Mikroskop för protein Imaging

This paper aims to instruct the reader in the assembly and operation of an infrared near-field microscope for imaging beyond the diffraction limit.  The ...

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Biology

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Imaging C. elegans Embryos using an Epifluorescent Microscope and Open Source Software

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Automated Hydrophobic Interaction Chromatography Column Selection for Use in Protein Purification

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Att detektera och karakterisera proteiners självmontering in vivo med flödescytometri

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