Name: noninvasive in vivo small animal mri and mrs: basic experimental procedures
Uploaded: 2009-10-20T14:00:00
Description: Watch this Scientific Journal Video about Noninvasive In Vivo Small Animal MRI and MRS: Basic Experimental Procedures at JoVE.com

这项研究是由美国国立卫生研究院/ NIBIB R21EB008166支持DL和NIA的AG028455和NIA的粉喷桩AG022385部分。我们感谢提供他们D282荷瘤小鼠的弗雷德哈钦森癌症研究中心的詹姆斯奥尔森博士。

第1部分：安全磁铁 MRI和MRS使用一个强大的磁场，需要格外小心。例如，仪器，我们目前的工作中使用的4.7 T磁场，地球磁场的约90000倍。高磁场始终是即使不被使用的磁共振扫描仪。任何金属物体接触到这样一个高磁场的强烈和迅速将磁铁吸引。这是极其危险的，如果一个实验题目或运营商是在位于飞入磁铁的金属对象的弹丸路径。因此，进行致辞实验的人员应小心删除任何?...

<ol>
	<li>Stecco, A., Saponaro, A., Carriero, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Patient+safety+issues+in+magnetic+resonance+imaging:+state+of+the+art.">Patient safety issues in magnetic resonance imaging: state of the art.</a> Radiol Med. 112, 491-491 (2007).</li><li>Heineman, F. W., Eng, J., Berkowitz, B. A., Balaban, R. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=NMR+spectral+analysis+of+kinetic+data+using+natural+lineshapes.">NMR spectral analysis of kinetic data using natural lineshapes.</a> Magn Reson Med. 13, 490-490 (1990).</li><li>Amara, C. E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Mitochondrial+function+in+vivo:+spectroscopy+provides+window+on+cellular+energetics.">Mitochondrial function in vivo: spectroscopy provides window on cellular energetics.</a> Methods. 46, 312-312 (2008).</li><li>Blei, M. L., Conley, K. E., Kushmerick, M. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Separate+measures+of+ATP+utilization+and+recovery+in+human+skeletal+muscle.">Separate measures of ATP utilization and recovery in human skeletal muscle.</a> J Physiol. 465, 203-203 (1993).</li><li>Marcinek, D. J., Schenkman, K. A., Ciesielski, W. A., Conley, K. E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Mitochondrial+coupling+in+vivo+in+mouse+skeletal+muscle.">Mitochondrial coupling in vivo in mouse skeletal muscle.</a> Am J Physiol Cell Physiol. 286, C457-C457 (2004).</li><li>Paganini, A. T., Foley, J. M., Meyer, R. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Linear+dependence+of+muscle+phosphocreatine+kinetics+on+oxidative+capacity.">Linear dependence of muscle phosphocreatine kinetics on oxidative capacity.</a> Am J Physiol. 272, 501-501 (1997).</li><li>Sun, C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=In+vivo+MRI+detection+of+gliomas+by+chlorotoxin-conjugated+superparamagnetic+nanoprobes.">In vivo MRI detection of gliomas by chlorotoxin-conjugated superparamagnetic nanoprobes.</a> Small. 4, 495-495 (2008).</li><li>Marcinek, D. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Reduced+mitochondrial+coupling+in+vivo+alters+cellular+energetics+in+aged+mouse+skeletal+muscle.">Reduced mitochondrial coupling in vivo alters cellular energetics in aged mouse skeletal muscle.</a> J Physiol. 569, 467-467 (2005).</li></ol>

调整/匹配和垫补收购前的步骤是至关重​​要的，以获得高空间分辨率和高的信号噪声比（SNR）。同样重要的是监测动物与动物监测系统，以保持在一个稳定的信号采集的生理状态的动物，符合人性化的动物处理，以防止任何潜在的artifactual测量条件。在协议解释的程序，可以进行修改，以获取更多的信息，包括在体内的扩散，灌注和血流成像和本地化光谱。所有的动物准备工作应该是相似的，?...

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<td>Inova 200 MR scanner/4.7 T</td>
<td>&nbsp;</td>
<td>Varian/Bruker</td>
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<td>Used for mouse MRI</td>
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<td>Inova 300 NMR spectrometer/7 T</td>
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<td>Varian/Oxford</td>
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<td>Used for MRS of mouse skeletal muscle</td>
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noninvasive in vivo small animal mri and mrs: basic experimental procedures

小动物磁共振（MR）的研究已成为现代生物医学研究，由于其非侵入性的性质和它提供了丰富的生物信息的重要元素。议员并不需要任何电离辐射，非侵入性，可以提供更高的分辨率和更好的信号噪声比在其他断层或光谱方式相比。在这个协议中，我们将重点放在小动物磁共振成像和磁共振波谱（MRI / MRS）的非侵入性收购放松加权<sup&gt; 1</sup&gt; H图像的​​鼠标，并获得<sup&gt; 31</sup&gt; P光谱小鼠肌肉。这项工作并不试图涵盖所有方面的小动物MRI /刘健，而是引入鼠标MRI /刘健实验的基本程序。这项工作的主要目标是告知研究人员的基本程序<em&gt;在体内</em&gt;致辞实验的小动物。我们的目标是提供一个更好地了解基本的实验程序，允许研究人员的新致辞领域，以更好地规划，使议员和非议员的程序无缝集成的研究非议员组成部分。

Watch this Scientific Journal Video about Noninvasive In Vivo Small Animal MRI and MRS: Basic Experimental Procedures at JoVE.com

Noninvasive In Vivo Small Animal MRI and MRS: Basic Experimental Procedures

这项工作描述无创性的小动物MRI和MRS的基本程序<em&gt;在体内</em&gt;。

无创在体内小动物的MRI和MRS：基本实验程序

Small animal Magnetic Resonance (MR) research has emerged as an important element of modern biomedical research due to its non-invasive nature and the ...

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Noninvasive In Vivo Small Animal MRI and MRS: Basic Experimental Procedures

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