Name: monitoring heart function in larval drosophila melanogaster for physiological studies
Uploaded: 2009-11-16T17:02:00
Duration: 9 min 37 s
Description: Watch this Scientific Journal Video about Monitoring Heart Function in Larval Drosophila melanogaster for Physiological Studies at JoVE.com

Le financement fourni par les Bourses Ribble G. dans le Département de biologie, les chercheurs du Kentucky et de jeunes du premier cycle d&#39;éducation-Eureka! Bureau (Univ. de KY).

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A: Molecular &#38; Integrative Physiol. 154, 173-183 (2009).</li><li>Ponzielli, R., Astier, M., Chartier, A., Gallet, A., Therond, P., Semeriva, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Heart+tube+patterning+in+Drosophila+requires+integration+of+axial+and+segmental+information+provided+by+the+Bithorax+Complex+genes+and+hedgehog+signaling.">Heart tube patterning in Drosophila requires integration of axial and segmental information provided by the Bithorax Complex genes and hedgehog signaling.</a> Develop. 129, 4509-4521 (2002).</li><li>Sl ma, K., Farkas, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Heartbeat+patterns+during+the+postembryonic+development+of+Drosophila+melanogaster.">Heartbeat patterns during the postembryonic development of Drosophila melanogaster.</a> J. 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<ol><li> Des outils de dissection: Beaux # 5 pinces et ciseaux fins (tous obtenus à partir des outils Fine Science (USA), Inc, 373-G Vintage Park Drive, Foster City, CA 94404-1139) </li><li> Loupe binoculaire avec fonction zoom pour la dissection et la fréquence cardiaque de comptage. Pour la stimulation focale du cœur cela est nécessaire aussi bien. </li><li> Pour les enregistrements extracellulaires et intracellulaires un microscope composé avec les objectifs debout (4 x et 20x) est utilisé. Standard amplificateur intracellulaire et A / D sur le conseil d&#39;administration de l&#39;enregistrement en ligne à un ordinateur. Les signaux électriques sont enregistrées sur la ligne à une interface PowerLab/4s (ADInstruments, Australie). Nous utilisons un logiciel standard de ADInstruments nommé Chart ou la portée. </li><li> Chimie: Tous les produits chimiques salins sont obtenus chez Sigma Chemical Company (St. Louis, MO). </li><li> Pour les enregistrements intracellulaires nous utilisons tube capillaire en verre (catalogue # 30-31-0 du FHC, Brunswick, ME, 04011, USA) et pour l&#39;enregistrement macropatch focale et électrodes de stimulation que nous utilisons Kimax-51, Kimble produits d&#39;art. N ° 34502, ID de 0,8 1,1 mm, longueur 100mm. L&#39;électrode intracellulaire doivent avoir une résistance de 20 à 30 Mohm. L&#39;électrode macropatch est construite par la rupture de la pointe du verre après une pointe fine a été faite à partir d&#39;un extracteur d&#39;électrode. La pointe hors cassée doit être une pause perpendiculaires propre sujet 20μM de diamètre. La pointe est alors de la chaleur à environ 10 uM polie diamètre intérieur pour l&#39;enregistrement de contact extracellulaire. Pour l&#39;électrode de stimulation de conduire le cœur, nous utilisons un bout final d&#39;environ 20-50 m de diamètre. L&#39;arbre de l&#39;électrode est exécuté sur un élément chauffant pour le faire plier d&#39;environ 45 degrés avec un coude progressif. Cela produit un plat ou d&#39;électrodes perpendiculaires lumière sur le tube cardiaque comme l&#39;angle avec le micro-manipulateur va produire sur une autre de 45 degrés à la préparation. </li></ol>

monitoring heart function in larval drosophila melanogaster for physiological studies

Nous présentons différentes méthodes pour enregistrer la fonction cardiaque chez la larve<em&gt; Drosophile</em&gt;. Les approches permettent de fréquence cardiaque pour être mesuré dans l&#39;ensemble des larves effrénée et sobre. Pour le contrôle direct de l&#39;environnement autour du cœur une autre approche utilise les larves disséquées et l&#39;enlèvement des organes internes afin de se baigner au cœur de composés désirés. Le cœur permet également exposés des potentiels de membrane à surveiller, qui peuvent donner un aperçu des courants ioniques générés par les myocytes et la conduction électrique le long du tube cardiaque. Ces approches ont des avantages et des inconvénients pour les futures expériences qui sont discutées. La préparation du cœur des larves fournit un modèle supplémentaire en plus du<em&gt; Drosophile</em&gt; Squelettiques JNM pour enquêter sur le rôle de la régulation du calcium intracellulaire sur la fonction cellulaire. En apprendre davantage sur les courants sous-jacents ioniques qui déterminent les potentiels d&#39;action dans les myocytes dans diverses espèces, on peut espérer obtenir une poignée sur les dysfonctionnements connus ioniques associés à des gènes spécifiques responsables de diverses maladies chez les mammifères.

Watch this Scientific Journal Video about Monitoring Heart Function in Larval Drosophila melanogaster for Physiological Studies at JoVE.com

Monitoring Heart Function in Larval Drosophila melanogaster for Physiological Studies

Nous présentons différentes manières de surveiller la fonction cardiaque chez la larve de<em&gt; Drosophile</em&gt; Pour l&#39;évaluation de questions ayant trait à la fonction de jonctions gap, des mutations des canaux ioniques, la modulation de l&#39;activité pacemaker et des études pharmacologiques.

Surveillance de la fonction cardiaque chez les larves Drosophila melanogaster Pour les études physiologiques

We present various methods to record cardiac function in the larval Drosophila. The approaches allow heart rate to be measured in unrestrained  and  ...

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Monitoring Heart Function in Larval Drosophila melanogaster for Physiological Studies

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