Name: monitoring heart function in larval drosophila melanogaster for physiological studies
Uploaded: 2009-11-16T17:02:00
Duration: 9 min 37 s
Description: Watch this Scientific Journal Video about Monitoring Heart Function in Larval Drosophila melanogaster for Physiological Studies at JoVE.com

Förderung durch das G. Ribble Fellowships in der Abteilung für Biologie, das Kentucky Young Researchers und Undergraduate Education-Eureka zur Verfügung gestellt! Office (Univ. of KY).

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<ol><li> Dissection-Tools: Fine Nr. 5 Pinzette und einer feinen Schere (alle von Fine Science Tools (USA), Inc., 373-G Vintage Park Drive, Foster City, CA 94404-1139 erhalten) </li><li> Präpariermikroskop mit Zoom-Funktion für die Präparation und das Zählen der Herzfrequenz. Für fokale Stimulation des Herzens ist dies auch notwendig. </li><li> Für extra-und intrazellulären Aufnahmen ein zusammengesetztes Mikroskop mit aufrechten Ziele (4 x und 20x) verwendet wird. Standard intrazellulären Verstärker und A / D-Karte für Online-Aufzeichnung auf einem Computer. Elektrische Signale werden auf Linie zu einem PowerLab/4s Schnittstelle (ADInstruments, Australien) aufgezeichnet. Wir verwenden Standard-Software von ADInstruments namens Chart-oder Scope. </li><li> Chemikalien: Alle Kochsalzlösung Chemikalien von Sigma Chemical Company (St. Louis, MO) erhalten. </li><li> Für intrazelluläre Ableitungen verwenden wir Glaskapillare Schlauch (Katalog # 30-31-0 von FHC, Brunswick, ME, 04011, USA) und für fokale macropatch Aufnahme und stimulierenden Elektroden verwenden wir Kimax-51, Kimble Products Kunst. Nr. 34502, ID 0,8-1.1mm, Länge 100mm. Die intrazelluläre Elektrode sollte einen Widerstand von 20 bis 30 mOhm. Die macropatch Elektrode wird durch den Abbruch der Spitze des Glases nach einer feinen Spitze von einer Elektrode Abzieher gemacht wurde gebaut. Die abgebrochene Spitze braucht, um eine saubere senkrecht zu brechen etwa 20 um im Durchmesser. Die Spitze wird dann Wärme poliert, um etwa 10 &amp;mgr; m Innendurchmesser für den Schwerpunkt extrazellulären Aufnahme. Für die Stimulationselektrode das Herz-Laufwerk verwenden wir eine abschließende Spitze der etwa 20-50 m im Durchmesser. Der Schaft der Elektrode ist über ein Heizelement ausführen, um dazu führen, dass etwa 45 Grad mit einer allmählichen Kurve biegen. Dadurch entsteht eine ebene oder senkrecht Elektrode Lumen über dem Herzen Rohr als der Winkel mit dem Mikro-Manipulator wird über weitere 45 Grad auf die Vorbereitung zu produzieren. </li></ol>

monitoring heart function in larval drosophila melanogaster for physiological studies

Wir präsentieren verschiedene Methoden, um die Herzfunktion in der Larven-Rekord<em&gt; Drosophila</em&gt;. Die Ansätze ermöglichen Herzfrequenz in hemmungsloser und zurückhaltend ganze Larven gemessen werden. Für die direkte Kontrolle über die Umgebung rund um das Herz anderer Ansatz nutzt die zergliedert Larven und Entfernung der inneren Organe, um die Herzen in der gewünschten Verbindungen zu baden. Die freiliegenden Herzen ermöglicht auch Membranpotentiale zu überwachenden welche Einblick in die Ionenströme durch die Myozyten und zur elektrischen Leitung entlang der Herzen Röhre erzeugt geben. Diese Ansätze haben verschiedene Vor-und Nachteile für zukünftige Experimente, die diskutiert werden. Die Larven Herzen Vorbereitung bietet ein zusätzliches Modell neben der<em&gt; Drosophila</em&gt; Skelett NMJ zu untersuchen, die Rolle der intrazellulären Calcium-Regulation der zellulären Funktion. Lernen Sie mehr über die zugrunde liegenden Ionenströme, dass die Aktionspotentiale Form in Myozyten in verschiedenen Arten, kann man hoffen, einen Griff an der bekannten ionischen Störungen verbunden, um spezifische Gene, die für verschiedene Krankheiten bei Säugetieren erhalten.

Watch this Scientific Journal Video about Monitoring Heart Function in Larval Drosophila melanogaster for Physiological Studies at JoVE.com

Monitoring Heart Function in Larval Drosophila melanogaster for Physiological Studies

Wir präsentieren verschiedene Wege, um die Herzfunktion in der Larve des Monitors<em&gt; Drosophila</em&gt; Für die Beurteilung der Fragen, die sich mit der Funktion von Gap Junctions, Ionenkanal-Mutationen, Modulation der Schrittmacher-Aktivität und pharmakologischen Studien.

Überwachung Herzfunktion Larven Drosophila melanogaster Für physiologische Untersuchungen

We present various methods to record cardiac function in the larval Drosophila. The approaches allow heart rate to be measured in unrestrained  and  ...

Research

JoVE Journal

Biology

Monitoring Heart Function in Larval Drosophila melanogaster for Physiological Studies

Dissection of Larval CNS in Drosophila Melanogaster

Dissection der Larven ZNS von Drosophila melanogaster

The central nervous system (CNS) of Drosophila larvae is complex and poorly understood.  One way to investigate the CNS is to use immunohistochemistry to ...

Forschung

Biologie

High-Resolution Video Tracking of Locomotion in Adult Drosophila Melanogaster

Hochauflösendes Video Tracking von Locomotion in Adult Drosophila melanogaster

Flies provide an important model for studying complex behavior due to the plethora of genetic tools available to researchers in this field.  Studying ...

Drosophila Larval NMJ Dissection

Drosophila Larven NMJ Dissection

The Drosophila neuromuscular junction (NMJ) is an established model system used for the study of synaptic development and plasticity. The widespread use ...

Drosophila Larval NMJ Immunohistochemistry

Drosophila Larval NMJ Immunohistochemistry

Drosophila Larven NMJ Immunhistochemie

Visually Mediated Odor Tracking During Flight in Drosophila

Visuell vermittelte Odor-Tracking während des Fluges in Drosophila

Flying insects use visual cues to stabilize their heading in a wind stream. Many animals additionally track odors carried in the wind. As such, visual ...

Visualizing the Beating Heart in Drosophila

Visualizing the Beating Heart in Drosophila

Visualisierung des schlagenden Herzens in Drosophila</em

The Drosophila heart has recently emerged as a good model system for examining the genetic, cellular, and molecular mechanisms underlying function in ...

Preparation of embryos for Electron Microscopy of the Drosophila embryonic heart tube

Preparation of embryos for Electron Microscopy of the Drosophila embryonic heart tube

Vorbereitung der Embryonen für Elektronenmikroskopie der Drosophila Embryonale Herz Rohr

The morphogenesis of the Drosophila  embryonic heart tube has emerged as a valuable model system for studying cell migration, cell-cell adhesion and cell ...

Heart Dissection in Larval, Juvenile and Adult Zebrafish, Danio rerio

Heart Dissection in Larval, Juvenile and Adult Zebrafish, Danio rerio

Herz Dissection in Larven, juvenile und adulte Zebrafisch, Danio rerio</em

Zebrafish have become a beneficial and practical model organism for the study of embryonic heart development (see recent reviews1-6), however, work ...

Measurement of Lifespan in Drosophila melanogaster

Measurement of Lifespan in Drosophila melanogaster

Messung der Lebensdauer in<em&gt; Drosophila melanogaster</em

Aging is a phenomenon that results in steady physiological deterioration in nearly all organisms in which it has been examined, leading to reduced ...

Drosophila Preparation and Longitudinal Imaging of Heart Function In Vivo Using Optical Coherence Microscopy (OCM)

Drosophila Preparation and Longitudinal Imaging of Heart Function In Vivo Using Optical Coherence Microscopy OCM

Drosophila Vorbereitung und Longitudinal Imaging der Herzfunktion in vivo unter Verwendung optischer Kohärenzmikroskopie (OCM)

Longitudinal study of the heartbeat in small animals contributes to understanding structural and functional changes during heart development. Optical ...