Name: monitoring heart function in larval drosophila melanogaster for physiological studies
Uploaded: 2009-11-16T17:02:00
Duration: 9 min 37 s
Description: Watch this Scientific Journal Video about Monitoring Heart Function in Larval Drosophila melanogaster for Physiological Studies at JoVE.com

Il finanziamento fornito dal borse Ribble G. nel Dipartimento di Biologia, il Kentucky Giovani Ricercatori universitari e Istruzione-eureka! Ufficio (Univ. di KY).

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<ol><li> Strumenti di dissezione: Fine # 5 pinzette e forbici fine (tutti ottenuti da Strumenti Scienze Fine (USA), Inc., 373-G Vintage Park Drive, Foster City, CA 94404-1139) </li><li> Dissezione microscopio con funzione di zoom per la dissezione e la frequenza cardiaca di conteggio. Per la stimolazione focale del cuore questo è necessario pure. </li><li> Per le registrazioni extracellulari e intracellulari un microscopio composto con obiettivi in ​​posizione verticale (4 x e 20X) viene utilizzato. Standard amplificatore intracellulare e A / D a bordo per la registrazione in linea a un computer. I segnali elettrici vengono registrati in linea ad una interfaccia PowerLab/4s (ADInstruments, Australia). Noi utilizziamo software standard da ADInstruments nome o Campo Grafico. </li><li> Prodotti chimici: Tutti i prodotti chimici saline sono ottenuti da Sigma Chemical Company (St. Louis, MO). </li><li> Per le registrazioni intracellulari usiamo tubo capillare di vetro (catalogo # 30-31-0 da FHC, Brunswick, ME, 04011, USA) e per la registrazione macropatch focali e gli elettrodi stimolanti usiamo Kimax-51, Kimble Art Prodotti. N. 34502, ID 0.8-1.1mm, lunghezza 100mm. L&#39;elettrodo intracellulare dovrebbe avere una resistenza di 20 a 30 mOhm. L&#39;elettrodo macropatch è costruito rompendo la punta di un vetro dopo una punta fine è stata fatta da un elettrodo estrattore. La punta fuori rotta deve essere una rottura perpendicolare pulito circa 20μM di diametro. La punta è poi il calore lucidato a circa 10μM diametro interno per la registrazione focale extracellulare. Per l&#39;elettrodo stimolante per guidare il cuore che usiamo una punta finale di circa 20-50 micron di diametro. L&#39;albero dell&#39;elettrodo viene investito un elemento riscaldante per indurla a piegarsi di circa 45 gradi con una curva graduale. Questo produce un appartamento o lume elettrodo perpendicolare sul tubo cuore come l&#39;angolo con il micro-manipolatore produrrà circa altri 45 gradi rispetto alla preparazione. </li></ol>

monitoring heart function in larval drosophila melanogaster for physiological studies

Vi presentiamo vari metodi per registrare la funzione cardiaca nei larvale<em&gt; Drosophila</em&gt;. Gli approcci permettono la frequenza cardiaca di essere misurato in sfrenato e trattenuto larve intero. Per il controllo diretto dell&#39;ambiente intorno al cuore un altro approccio sfrutta le larve sezionato e la rimozione degli organi interni in modo da bagnare il cuore in composti desiderato. Il cuore esposto consente inoltre di potenziali di membrana da monitorare che può dare comprensione delle correnti ioniche generate dal miociti e di conduzione elettrica lungo il tubo cuore. Questi approcci hanno vantaggi e svantaggi per gli esperimenti futuri che sono discussi. La preparazione cuore larvale fornisce un modello aggiuntivo oltre ai<em&gt; Drosophila</em&gt; Scheletrico NMJ per indagare il ruolo della regolazione del calcio intracellulare sulla funzione cellulare. Conoscere meglio le correnti ioniche di fondo che danno forma al potenziali d&#39;azione in miociti in varie specie, si può sperare di ottenere un handle sul disfunzioni noto ionici associati a specifici geni responsabili di varie malattie nei mammiferi.

Watch this Scientific Journal Video about Monitoring Heart Function in Larval Drosophila melanogaster for Physiological Studies at JoVE.com

Monitoring Heart Function in Larval Drosophila melanogaster for Physiological Studies

Vi presentiamo vari modi per monitorare la funzione cardiaca in larva di<em&gt; Drosophila</em&gt; Per valutare le domande che fare con la funzione delle giunzioni gap, le mutazioni dei canali ionici, modulazione dell&#39;attività pacemaker e degli studi farmacologici.

Il monitoraggio delle funzioni cardiaca in larvali Drosophila melanogaster Per gli Studi fisiologici

We present various methods to record cardiac function in the larval Drosophila. The approaches allow heart rate to be measured in unrestrained  and  ...

Research

JoVE Journal

Biology

Monitoring Heart Function in Larval Drosophila melanogaster for Physiological Studies

Dissection of Larval CNS in Drosophila Melanogaster

Dissezione del sistema nervoso centrale larvali in Drosophila melanogaster

The central nervous system (CNS) of Drosophila larvae is complex and poorly understood.  One way to investigate the CNS is to use immunohistochemistry to ...

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Biologia

High-Resolution Video Tracking of Locomotion in Adult Drosophila Melanogaster

Monitoraggio ad alta risoluzione video di locomozione per adulti in Drosophila melanogaster

Flies provide an important model for studying complex behavior due to the plethora of genetic tools available to researchers in this field.  Studying ...

Drosophila Larval NMJ Dissection

Drosophila larvali NMJ Dissection

The Drosophila neuromuscular junction (NMJ) is an established model system used for the study of synaptic development and plasticity. The widespread use ...

Drosophila Larval NMJ Immunohistochemistry

Drosophila Larval NMJ Immunohistochemistry

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Visivamente monitoraggio Odore mediata durante il volo in Drosophila

Flying insects use visual cues to stabilize their heading in a wind stream. Many animals additionally track odors carried in the wind. As such, visual ...

Visualizing the Beating Heart in Drosophila

Visualizing the Beating Heart in Drosophila

Visualizzare il cuore pulsante di Drosophila</em

The Drosophila heart has recently emerged as a good model system for examining the genetic, cellular, and molecular mechanisms underlying function in ...

Preparation of embryos for Electron Microscopy of the Drosophila embryonic heart tube

Preparation of embryos for Electron Microscopy of the Drosophila embryonic heart tube

Preparazione di embrioni per la microscopia elettronica del Drosophila Tubo cuore embrionale

The morphogenesis of the Drosophila  embryonic heart tube has emerged as a valuable model system for studying cell migration, cell-cell adhesion and cell ...

Heart Dissection in Larval, Juvenile and Adult Zebrafish, Danio rerio

Heart Dissection in Larval, Juvenile and Adult Zebrafish, Danio rerio

Dissezione di cuore in larvale, Zebrafish giovanile e degli adulti, Danio rerio</em

Zebrafish have become a beneficial and practical model organism for the study of embryonic heart development (see recent reviews1-6), however, work ...

Measurement of Lifespan in Drosophila melanogaster

Measurement of Lifespan in Drosophila melanogaster

Misura della durata della vita in<em&gt; Drosophila melanogaster</em

Aging is a phenomenon that results in steady physiological deterioration in nearly all organisms in which it has been examined, leading to reduced ...

Drosophila Preparation and Longitudinal Imaging of Heart Function In Vivo Using Optical Coherence Microscopy (OCM)

Drosophila Preparation and Longitudinal Imaging of Heart Function In Vivo Using Optical Coherence Microscopy OCM

Drosophila preparazione e imaging longitudinale della funzione cardiaca in vivo utilizzando Optical Coherence Microscopy (OCM)

Longitudinal study of the heartbeat in small animals contributes to understanding structural and functional changes during heart development. Optical ...