Name: monitoring heart function in larval drosophila melanogaster for physiological studies
Uploaded: 2009-11-16T17:02:00
Duration: 9 min 37 s
Description: Watch this Scientific Journal Video about Monitoring Heart Function in Larval Drosophila melanogaster for Physiological Studies at JoVE.com

生物学の部門でのG.のリブルフェローシップ、ケンタッキー州若手研究者と学部教育=ユリーカで提供される資金調達！事務所（ケンタッキー大）。

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<ol><li>解剖ツール：ファイン＃5ピンセットと（すべてのファイン科学のツール（米国）、（株）、373 - Gビンテージパークドライブ、フォスターシティー、CA 94404から1139から得られる）罰金はさみ</li><li>解剖と計数心拍数のためのズーム機能で顕微鏡を解剖。心臓の局所刺激のためにこれも必要です。 </li><li>細胞外および細胞内記録のために直立の目標（4 ×と20倍）を持つ化合物の顕微鏡が使用されます。 コンピュータへのライン録音での標準的な細胞内のアンプとA / Dボード。電気信号は、PowerLab/4sインタフェース（ADInstruments、オーストラリア）への行に記録されます。我々は、チャートまたはScopeというADInstrumentsから標準のソフトウェアを使用してください。 </li><li>化学物質：すべての生理食塩水の化学物質は、シグマケミカル社（セントルイス、MO）から入手されています。 </li><li>細胞内記録のための我々はガラスキャピラリチューブ（FHC、ブランズウィック、ME、04011、アメリカ合衆国カタログ＃30-31-0）を使用すると焦点macropatchの記録と刺激電極のために我々はKimax - 51を使用して、キンブルプロダクトアート。 100ミリメートル番号34502、ID 0.8〜1.1ミリメートル、長さ。細胞内電極は、20〜30オームの抵抗を持つ必要があります。 macropatch電極は、微細な先端が電極の引き手から作られた後のガラスの先端を切り離すことにより構築されます。壊れたオフの先端の直径は20μM程度きれいな垂直のブレークにする必要があります。ヒントは、焦点外記録用10μM約内径に研磨熱である。心臓を駆動するために刺激電極のために我々は、直径約20〜50μMの最後のヒントを使用してください。電極のシャフトは、それが徐々に曲げて約45度に曲げるために発生する発熱体を介して実行されます。これは準備にさらに45度程度が生成されますマイクロマニピュレータを持つ角度など、心臓の管がフラットまたは垂直電極の内腔を生成します。 </li></ol>

monitoring heart function in larval drosophila melanogaster for physiological studies

私たちは、幼虫の心臓機能を記録するためにさまざまな方法を提示<em&gt;ショウジョウバエ</em&gt;。アプローチは、心拍数が無制限と控えめ全体幼虫で測定することができます。心臓の周りの環境を直接制御するための別のアプローチは、所望の化合物に心を入浴するために切開幼虫や内臓の除去を利用しています。露出の心も膜電位が心筋細胞で、心臓の管に沿って電気伝導のために生成されたイオン電流の洞察力を与えることができる監視することができます。これらのアプローチは説明されている将来の実験のための様々な長所と短所があります。幼虫の心の準備は、ほかに追加のモデルが用意されています<em&gt;ショウジョウバエ</em細胞機能に対する細胞内カルシウム調節の役割を調査するために&gt;骨格NMJ。様々な種における筋細胞における活動電位を形作る基本的なイオン電流をより深く学ぶ、人は哺乳類の様々な疾患の原因特異的な遺伝子に関連する既知のイオン性機能障害のハンドルを取得するために期待できます。

Watch this Scientific Journal Video about Monitoring Heart Function in Larval Drosophila melanogaster for Physiological Studies at JoVE.com

Monitoring Heart Function in Larval Drosophila melanogaster for Physiological Studies

我々はの幼虫に心機能を監視するためにさまざまな方法を提示<em&gt;ショウジョウバエ</em&gt;ギャップ結合、イオンチャネルの変異が、ペースメーカー活性の調節と薬理学的研究の機能を扱うの質問を評価する。

幼虫のモニタリングの心機能キイロショウジョウバエ生理学的研究のための

We present various methods to record cardiac function in the larval Drosophila. The approaches allow heart rate to be measured in unrestrained  and  ...

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Biology

Monitoring Heart Function in Larval Drosophila melanogaster for Physiological Studies

Dissection of Larval CNS in Drosophila Melanogaster

キイロショウジョウバエの幼虫中枢神経系の解剖

The central nervous system (CNS) of Drosophila larvae is complex and poorly understood.  One way to investigate the CNS is to use immunohistochemistry to ...

High-Resolution Video Tracking of Locomotion in Adult Drosophila Melanogaster

アダルトキイロショウジョウバエのロコモーションの高解像度のビデオトラッキング

Flies provide an important model for studying complex behavior due to the plethora of genetic tools available to researchers in this field.  Studying ...

Drosophila Larval NMJ Dissection

ショウジョウバエ幼虫NMJの解剖

The Drosophila neuromuscular junction (NMJ) is an established model system used for the study of synaptic development and plasticity. The widespread use ...

Drosophila Larval NMJ Immunohistochemistry

Drosophila Larval NMJ Immunohistochemistry

ショウジョウバエ幼虫NMJ免疫組織化学

Visually Mediated Odor Tracking During Flight in Drosophila

ショウジョウバエのフライトの間に視覚的に仲介臭気の追跡

Flying insects use visual cues to stabilize their heading in a wind stream. Many animals additionally track odors carried in the wind. As such, visual ...

Visualizing the Beating Heart in Drosophila

Visualizing the Beating Heart in Drosophila

の心臓の鼓動の可視化ショウジョウバエ</em

The Drosophila heart has recently emerged as a good model system for examining the genetic, cellular, and molecular mechanisms underlying function in ...

Preparation of embryos for Electron Microscopy of the Drosophila embryonic heart tube

Preparation of embryos for Electron Microscopy of the Drosophila embryonic heart tube

の電子顕微鏡のための胚の調製ショウジョウバエ胎児心臓の管

The morphogenesis of the Drosophila  embryonic heart tube has emerged as a valuable model system for studying cell migration, cell-cell adhesion and cell ...

Heart Dissection in Larval, Juvenile and Adult Zebrafish, Danio rerio

Heart Dissection in Larval, Juvenile and Adult Zebrafish, Danio rerio

幼虫の心臓の解剖、少年と大人ゼブラフィッシュ、ゼブラフィッシュ</em

Zebrafish have become a beneficial and practical model organism for the study of embryonic heart development (see recent reviews1-6), however, work ...

Measurement of Lifespan in Drosophila melanogaster

Measurement of Lifespan in Drosophila melanogaster

における寿命の測定<em&gt;キイロショウジョウバエ</em

Aging is a phenomenon that results in steady physiological deterioration in nearly all organisms in which it has been examined, leading to reduced ...

Drosophila Preparation and Longitudinal Imaging of Heart Function In Vivo Using Optical Coherence Microscopy (OCM)

Drosophila Preparation and Longitudinal Imaging of Heart Function In Vivo Using Optical Coherence Microscopy OCM

ショウジョウバエの調製と光コヒーレンス顕微鏡を用いてin vivoで心機能の縦イメージング（OCM）

Longitudinal study of the heartbeat in small animals contributes to understanding structural and functional changes during heart development. Optical ...