Name: monitoring heart function in larval drosophila melanogaster for physiological studies
Uploaded: 2009-11-16T17:02:00
Duration: 9 min 37 s
Description: Watch this Scientific Journal Video about Monitoring Heart Function in Larval Drosophila melanogaster for Physiological Studies at JoVE.com

생물의 도서관, 켄터키 영 연구자 및 대학 교육 유레카의 G.의 리블 장학금 기금에 의해 제공! 오피스 (KY의 Univ.).

<ol>
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<ol><li> 해부 도구 : 좋아요 # 5 핀셋과 (모든 파인 과학 도구 (미국), 주식 회사, 373 - G 빈티지 파크 드라이브, 포스터 시티, CA 94404-1139에서 얻은) 고급 가위 </li><li> 해부 및 계산 심장 박동을위한 줌 기능과 함께 현미경을 해부. 심장의 자극에 초점 이것은뿐만 아니라 필요합니다. </li><li> 세포외 및 세포내 레코딩을위한 직립 목표 (4 x와 20X)와 복합 현미경이 사용됩니다. 표준 세포 증폭기와 컴퓨터에 라인 녹음에 대해서도 A / D 보드. 전기 신호는 PowerLab/4s 인터페이스 (ADInstruments, 호주)에 온라인으로 기록됩니다. 우리는 차트 또는 범위 이름을 ADInstruments에서 표준 소프트웨어를 사용합니다. </li><li> 화학 물질 : 모든 생리 화학 물질 시그마 화학 회사 (세인트 루이스, MO)에서 얻을 수 있습니다. </li><li> 세포내 녹음을 위해 우리는 유리 모세관 튜브 (FHC, 브런즈윅, ME, 04011, 미국의 카탈로그 # 30-31-0)과 초점 macropatch 녹음 그리고 우리가 Kimax - 51을 사용하여 자극 전극, 킴블 제품 아트를 사용합니다. 100mm 번호 34502, ID 0.8 - 1.1mm, 길이. 세포내 전극은 20-30 mOhm의 저항이 있어야합니다. macropatch 전극은 좋은 팁이 전극 풀러에서 만든 후 유리의 끝을 위반하여 구성되어 있습니다. 깨진 해제 팁 직경은 20μM에 대한 깨끗한 수직 휴식이 필요합니다. 팁 다음 초점 세포 녹음 10μM에 대한 내경을 연마 열 수 있습니다. 마음을 드라이브에 자극 전극을 위해 우리는 직경 20-50 μm의 약의 마지막 팁을 사용합니다. 전극의 샤프트는 그것이 점진적으로 구부 러와 함께 약 45 도로 구부 원인 가열 요소를 통해 실행됩니다. 이것은 준비하는 또 다른 45도에 대한 생산되는 마이크로 조작하는 사람과 각도로 심장 관을 통해 평면 또는 수직 전극 루멘을 생산하고 있습니다. </li></ol>

monitoring heart function in larval drosophila melanogaster for physiological studies

우리는 애벌레에서 심장 기능을 기록하는 다양한 방법을 제시<em&gt; Drosophila</em&gt;. 접근 방식은 심장 박동이 억제 및 억제 전체 애벌레에서 측정 수 있습니다. 심장 주변의 환경을 직접 제어를위한 또 다른 방법은 원하는 화합물의 마음을 목욕하기 위해 해부 애벌레와 내장 제거를 사용합니다. 노출 심장도 막 잠재력이 myocytes에 의해 및 심장 관을 따라 전기 전도에 대해 생성된 이온 전류의 통찰력을 줄 수있는 모니터링할 수 있습니다. 이러한 방식은 논의 이후의 실험을위한 다양한 장점과 단점이 있습니다. 애벌레 심장 준비가 외에 추가 모델을 제공합니다<em&gt; Drosophila</em&gt; 골격 NMJ는 세포 기능에 세포 내 칼슘 조절의 역할을 조사합니다. 더 다양한 수종의 myocytes의 행동 잠재력을 형성 기본 이온 전류에 대한 학습, 하나는 포유류의 다양한 질환에 대한 책임 특정 유전자에 관련된 알려진 이온 장애에 대한 핸들을 얻을 수 있도록 노력하겠습니다하실 수 있습니다.

Watch this Scientific Journal Video about Monitoring Heart Function in Larval Drosophila melanogaster for Physiological Studies at JoVE.com

Monitoring Heart Function in Larval Drosophila melanogaster for Physiological Studies

우리의 유충에서 심장 기능을 모니터링하는 여러 가지 방법을 제시<em&gt; Drosophila</em&gt; 간격 분기점, 이온 채널 돌연변이, 맥박 조정기 활동의 변조 및 약리 연구의 기능을 다루는 질문을 평가하십시오.

애벌레의 모니터링 심장​​ 기능 Drosophila melanogaster 생리 연구

We present various methods to record cardiac function in the larval Drosophila. The approaches allow heart rate to be measured in unrestrained  and  ...

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Biology

Monitoring Heart Function in Larval Drosophila melanogaster for Physiological Studies

Dissection of Larval CNS in Drosophila Melanogaster

Drosophila Melanogaster에서 애벌레 CNS의 해부

The central nervous system (CNS) of Drosophila larvae is complex and poorly understood.  One way to investigate the CNS is to use immunohistochemistry to ...

연구

생물학

High-Resolution Video Tracking of Locomotion in Adult Drosophila Melanogaster

성인 Drosophila Melanogaster의 로코의 고해상도 비디오 추적

Flies provide an important model for studying complex behavior due to the plethora of genetic tools available to researchers in this field.  Studying ...

Drosophila Larval NMJ Dissection

Drosophila 애벌레 NMJ의 해부

The Drosophila neuromuscular junction (NMJ) is an established model system used for the study of synaptic development and plasticity. The widespread use ...

Drosophila Larval NMJ Immunohistochemistry

Drosophila Larval NMJ Immunohistochemistry

Drosophila 애벌레 NMJ Immunohistochemistry

Visually Mediated Odor Tracking During Flight in Drosophila

Drosophila의 비행 중 시각 인한 악취 추적

Flying insects use visual cues to stabilize their heading in a wind stream. Many animals additionally track odors carried in the wind. As such, visual ...

Visualizing the Beating Heart in Drosophila

Visualizing the Beating Heart in Drosophila

에 잔 심장을 떠올리 Drosophila</em

The Drosophila heart has recently emerged as a good model system for examining the genetic, cellular, and molecular mechanisms underlying function in ...

Preparation of embryos for Electron Microscopy of the Drosophila embryonic heart tube

Preparation of embryos for Electron Microscopy of the Drosophila embryonic heart tube

의 전자 현미경을위한 배아의 준비 Drosophila 태아의 심장 관

The morphogenesis of the Drosophila  embryonic heart tube has emerged as a valuable model system for studying cell migration, cell-cell adhesion and cell ...

Heart Dissection in Larval, Juvenile and Adult Zebrafish, Danio rerio

Heart Dissection in Larval, Juvenile and Adult Zebrafish, Danio rerio

애벌레의 심장 해부, 소년과 성인 Zebrafish, Danio rerio</em

Zebrafish have become a beneficial and practical model organism for the study of embryonic heart development (see recent reviews1-6), however, work ...

Measurement of Lifespan in Drosophila melanogaster

Measurement of Lifespan in Drosophila melanogaster

의 수명 측정<em&gt; Drosophila melanogaster</em

Aging is a phenomenon that results in steady physiological deterioration in nearly all organisms in which it has been examined, leading to reduced ...

Drosophila Preparation and Longitudinal Imaging of Heart Function In Vivo Using Optical Coherence Microscopy (OCM)

Drosophila Preparation and Longitudinal Imaging of Heart Function In Vivo Using Optical Coherence Microscopy OCM

초파리 준비 및 빛 간섭 현미경을 사용하여 생체 내에서 심장 기능의 세로 이미징 (OCM)

Longitudinal study of the heartbeat in small animals contributes to understanding structural and functional changes during heart development. Optical ...

애벌레의 모니터링 심장​​ 기능 Drosophila melanogaster 생리 연구

애벌레의 모니터링 심장 기능 Drosophila melanogaster 생리 연구