Name: monitoring heart function in larval drosophila melanogaster for physiological studies
Uploaded: 2009-11-16T17:02:00
Duration: 9 min 37 s
Description: Watch this Scientific Journal Video about Monitoring Heart Function in Larval Drosophila melanogaster for Physiological Studies at JoVE.com

Financiamento fornecido pelo Bolsas Ribble G. no Departamento de Biologia, os pesquisadores Kentucky Jovem e Educação de Graduação-Eureka! Office (Univ. de KY).

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<ol><li> Ferramentas de dissecção: Fine # 5 pinças e tesouras finas (todos obtidos a partir de Ferramentas Ciência Fine (EUA), Inc., 373-G Vintage Park Drive, Foster City, CA 94404-1139) </li><li> Dissecando microscópio com função de zoom para dissecção e freqüência cardíaca de contagem. Focal para a estimulação do coração que isto é necessário também. </li><li> Para gravações extracelular e intracelular um microscópio composto com os objetivos de pé (4 x e 20x) é usado. Amplificador intracelular padrão e A / D placa para gravação em linha para um computador. Sinais elétricos são registrados na linha de uma interface PowerLab/4s (ADInstruments, Austrália). Nós usamos software padrão de ADInstruments chamado gráfico ou Âmbito. </li><li> Produtos químicos: Todos os produtos químicos salinos são obtidos da Sigma Chemical Company (St. Louis, MO). </li><li> Para gravações intracelular usamos um tubo de vidro capilar (30-31-0 catalogo de FHC, Brunswick, ME, 04011, EUA) e para a gravação macropatch focal e eletrodos de estimulação usamos Kimax-51, Kimble Art Products. N º 34.502, ID 0,8 1,1 milímetros, comprimento 100mm. O eletrodo intracelular deve ter uma resistência de 20 a 30 mOhm. O eletrodo é construído por macropatch quebrando a ponta do vidro após uma ponta fina foi feita a partir de um extrator de eletrodo. A ponta fora quebrado precisa ser uma ruptura perpendicular limpo sobre 20Hm de diâmetro. A dica é, então, o calor polido para cerca de 10μM diâmetro interno para a gravação focal extracelular. Para o eletrodo estimulando a conduzir o coração usamos uma ponta final de cerca de 20-50 m de diâmetro. O eixo do eletrodo é executado ao longo de um elemento de aquecimento para fazer com que ele dobre cerca de 45 graus com uma curvatura gradual. Isso produz um apartamento ou lúmen eletrodo perpendicular ao longo do tubo de coração como o ângulo com o micro-manipulador irá produzir cerca de mais 45 graus para a preparação. </li></ol>

monitoring heart function in larval drosophila melanogaster for physiological studies

Apresentamos vários métodos para gravar a função cardíaca no larval<em&gt; Drosophila</em&gt;. As abordagens permitem que a freqüência cardíaca a ser medido em larvas sem restrição todo. Para o controle direto do ambiente em torno do coração outra abordagem utiliza as larvas dissecadas e remoção dos órgãos internos, a fim de banhar o coração em compostos desejados. O coração exposto também permite que os potenciais de membrana para ser monitorado e que pode dar uma visão das correntes iônicas geradas pelo miócitos e para condução elétrica do coração ao longo do tubo. Essas abordagens têm várias vantagens e desvantagens para futuros experimentos que são discutidos. A preparação do coração larval fornece um modelo adicional além do<em&gt; Drosophila</em&gt; Esqueléticos MNJ para investigar o papel da regulação de cálcio intracelular na função celular. Aprender mais sobre as correntes subjacentes iônico que forma os potenciais de ação em miócitos em várias espécies, pode-se esperar obter uma alça sobre as disfunções conhecida iônicos associados a genes específicos responsáveis ​​por várias doenças em mamíferos.

Watch this Scientific Journal Video about Monitoring Heart Function in Larval Drosophila melanogaster for Physiological Studies at JoVE.com

Monitoring Heart Function in Larval Drosophila melanogaster for Physiological Studies

Apresentamos várias maneiras de monitorar a função cardíaca na larva de<em&gt; Drosophila</em&gt; Para avaliar questões relacionadas com a função de junções, mutações canal iônico, modulação da atividade de marcapasso e estudos farmacológicos.

Função de monitorização cardíaca em larval Drosophila melanogaster Para estudos fisiológicos

We present various methods to record cardiac function in the larval Drosophila. The approaches allow heart rate to be measured in unrestrained  and  ...

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Monitoring Heart Function in Larval Drosophila melanogaster for Physiological Studies

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Visualizar o coração batendo no Drosophila</em

The Drosophila heart has recently emerged as a good model system for examining the genetic, cellular, and molecular mechanisms underlying function in ...

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Preparation of embryos for Electron Microscopy of the Drosophila embryonic heart tube

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The morphogenesis of the Drosophila  embryonic heart tube has emerged as a valuable model system for studying cell migration, cell-cell adhesion and cell ...

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Dissecção do coração em larval, Zebrafish Juvenil e Adulto, Danio rerio</em

Zebrafish have become a beneficial and practical model organism for the study of embryonic heart development (see recent reviews1-6), however, work ...

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Measurement of Lifespan in Drosophila melanogaster

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Aging is a phenomenon that results in steady physiological deterioration in nearly all organisms in which it has been examined, leading to reduced ...

Drosophila Preparation and Longitudinal Imaging of Heart Function In Vivo Using Optical Coherence Microscopy (OCM)

Drosophila Preparation and Longitudinal Imaging of Heart Function In Vivo Using Optical Coherence Microscopy OCM

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Longitudinal study of the heartbeat in small animals contributes to understanding structural and functional changes during heart development. Optical ...