Name: dissection of 6.5 dpc mouse embryos
Uploaded: 2007-02-25T00:00:00
Description: Watch this Scientific Journal Video about Dissection of 6.5 dpc Mouse Embryos at JoVE.com

<p class="jove_step">从雌性小鼠生殖器官的解剖</p><ol><li>怀孕的动物安乐死的CO <sub>2，</sub>使用压缩气体窒息。 </li><li>莱吸水垫的动物仰卧浸泡在70％的乙醇，以减少污染解剖与鼠毛的风险。 </li><li>捏皮肤，做一个小切口与常规手术剪刀中线外侧。牢牢把握的上方和下方的切口的皮肤，除了拉向头部和尾部的皮肤暴露腹部。 </li><li>掌握与钳腹膜切开暴露腹腔。 </li><li>在体腔的背地区找到生殖器官：两个子宫角，输卵?...

<ol>
	<li>Nagy, A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> <em style='font-style: italic'>Manipulating the mouse embryo: A laboratory manual third edition</em>. , (2003).</li></ol>

<p>在小鼠植入后发生4.5 DPC和出生。 ，本质上描述的那样，这夹层的方法可应用于隔离较早或较晚的阶段胚胎在5.5-8.5 DPC。 </p>

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		<th>Material Name</th>
		<th>Type</th>
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		<th>Catalogue Number</th>
		<th>Comment</th>
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<td>Micro Dissecting Forceps serrated, straight</td>
<td>Tool</td>
<td>Roboz</td>
<td>RS-5130 </td>
<td>2 pairs</td>
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<tr>
<td>Micro Dissecting Tweezers Pattern #55 INOX</td>
<td>Tool</td>
<td>Roboz</td>
<td>RS-5010 </td>
<td>2 pairs</td>
</tr>
<tr>
<td>Dulbecco's Phosphate Buffered Saline (1X) </td>
<td>Reagent</td>
<td>Invitrogen</td>
<td>14190 </td>
<td>&nbsp;</td>
<tr>
<td>Fisherbrand Sterile Polysytrene Petri Dish</td>
<td>Tool</td>
<td>Fisher Scientific</td>
<td>08-757-12 </td>
<td>&nbsp;</td>
<tr>
<td>4% Paraformaldehyde in PBS</td>
<td>Reagent</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<tr>
<td>Straight Sharp-Pointed Dissecting Scissors</td>
<td>Tool</td>
<td>Fisher Scientific</td>
<td>08-940 </td>
<td>&nbsp;</td>
<tr>
<td>Pregnant female mice </td>
<td>Animal</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<tr>
<td>Dissecting Microscope</td>
<td>Microscope</td>
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dissection of 6.5 dpc mouse embryos

<p class="jove_content">在哺乳动物胚胎发育的早期阶段中基因表达模式的分析可以提供有关基因的功能，细胞与细胞间的相互作用和信号传导机制，引导胚胎图案的重要线索。然而，从小鼠胚胎植入后不久蜕膜剥离可以是一个具有挑战性的的过程，缺乏详细的一步一步的这个过程中的文档。 </p><p class="jove_content">在这里，我们将演示如何植入后（6.5 DPC）的胚胎首先剖析了孕鼠的子宫（阴道塞被指定每天0.5 poist coitum检测），随后解剖胚胎从母体蜕膜的隔离。赖克特的膜的夹层以及拆除ectoplacental锥描述。 </p>

Watch this Scientific Journal Video about Dissection of 6.5 dpc Mouse Embryos at JoVE.com

Dissection of 6.5 dpc Mouse Embryos

<p class="jove_content">植入后胚胎的隔离允许一个研究基因的图案和分析细胞系的胚胎发育过程中的决策过程，但早期胚胎的正常清​​扫，可以是具有挑战性的。这个协议描述为一个方法，隔离早期胚胎原始连胜阶段（〜6.5天coitum [DPC]）。</p>

6.5 DPC小鼠胚胎剖析

Analysis of gene expression patterns during early stages of mammalian embryonic development can provide important clues about gene function, cell-cell ...

Research

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Dissection of Organizer and Animal Pole Explants from Xenopus laevis Embryos and Assembly of a Cell Adhesion Assay

组织者和动物极植从非洲爪蟾胚胎和装配一个细胞粘附实验的剖析

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In Utero Intraventricular Injection and Electroporation of E15 Mouse Embryos

E15小鼠胚胎在子宫内侧脑室注射和电穿孔

In-utero in-vivo injection and electroporation of the embryonic mouse neocortex provides a powerful tool for the manipulation of individual progenitors ...

Collection and Cryopreservation of Hamster Oocytes and Mouse Embryos

收集和仓鼠卵母细胞和小鼠胚胎冷冻保存

Embryos and oocytes were first successfully cryopreserved more than 30 years ago, when Whittingham et al. 1 and Wilmut 2 separately described that mouse ...

Dissection of Hippocampal Dentate Gyrus from Adult Mouse

从成年小鼠海马齿状回的剖析

The hippocampus is one of the most widely studied areas in the brain because of its important functional role in memory processing and learning, its ...

Live Dissection of <em>Drosophila</em> Embryos: Streamlined Methods for Screening Mutant Collections by Antibody Staining

Live Dissection of Drosophila Embryos: Streamlined Methods for Screening Mutant Collections by Antibody Staining

现场剖析<em>果蝇</em>胚胎抗体染色筛选突变体集合的简化方法

Drosophila embryos between stages 14 and 17 of embryonic development can be readily dissected to generate "fillet" preparations.  In these preparations, ...

<em>In vivo</em> Imaging and Therapeutic Treatments in an Orthotopic Mouse Model of Ovarian Cancer

In vivo Imaging and Therapeutic Treatments in an Orthotopic Mouse Model of Ovarian Cancer

<em>活体</em>成像和治疗卵巢癌的原位小鼠模型

Human cancer and response to therapy is better represented in orthotopic animal models.  This paper describes the development of an orthotopic mouse model ...

Whole Mount <em>in Situ</em> Hybridization of E8.5 to E11.5 Mouse Embryos

Whole Mount in Situ Hybridization of E8.5 to E11.5 Mouse Embryos

全山<em>原位</em>杂交E8.5，E11.5小鼠胚胎

Whole mount in situ hybridization is a very informative approach for defining gene expression patterns in embryos. The in situ hybridization procedures ...

Cryopreservation of Mouse Embryos by Ethylene Glycol-Based Vitrification

基于乙二醇玻璃化冷冻小鼠胚胎的超低温保存

Cryopreservation of mouse embryos is a technological basis that supports biomedical sciences, because many strains of mice have been produced by genetic ...

Dissection and Downstream Analysis of Zebra Finch Embryos at Early Stages of Development

解剖和斑马雀胚胎下游分析在发展的早期阶段

The zebra finch (Taeniopygiaguttata) has become an increasingly important model organism in many areas of research including toxicology1,2, behavior3, and ...

Trans-inner Cell Mass Injection of Embryonic Stem Cells Leads to Higher Chimerism Rates

胚胎干细胞的跨内细胞质量注射导致嵌合体率增高

In an effort to increase efficiency in the creation of genetically modified mice via ES Cell methodologies, we present an adaptation to the current ...