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DOI :
10.3791/1614-v
•
13:39 min
December 10th, 2009
Chapters
0:00
Title
0:55
Introduction
1:39
Preparation for Injection and Fabrication of Injection Pipettes
12:00
Whole-cell Recordings of Currents through Heterologously-Expressed G-protein Coupled Inwardly Rectifying K+ (GIRK) Channels from SCG Neurons
4:54
Intranuclear Microinjection
12:56
Conclusion
cDNAの直接核内注入は、有糸分裂後の細胞のための効果的なトランスフェクションの手法です。このメソッドは、単一または複数のcDNA構築物から異種タンパク質の発現の高いレベルを提供し、単一細胞アッセイの様々な生理学的に適切な環境で検討するタンパク質の機能を有効にします。
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