Name: osmotic avoidance in caenorhabditis elegans: synaptic function of two genes, orthologues of human nrxn1 and nlgn1, as candidates for autism
Uploaded: 2009-12-11T16:00:00
Description: Watch this Scientific Journal Video about Osmotic Avoidance in Caenorhabditis elegans: Synaptic Function of Two Genes, Orthologues of Human NRXN1 and NLGN1, as Candidates for Autism at JoVE.com

我们想感谢他的宝贵帮助医生安东尼米兰达Vizuete。我们也想表达我们的宝贵的技术援助表示感谢萨尔玛Boulayoune和伊莎贝尔卡瓦列罗。这项工作是由军政府安达卢西亚（BIO - 272）的赠款资助。目前在欧洲的遗传实验的法律协议，这项研究已经进行了。

1：渗透避免检测。 <ol><li>检测前约16-24小时，挑到一个新的NGM板含有OP50 大肠杆菌 L4各基因型的幼虫阶段动物大肠杆菌 andincubate在20 ° C第二天，开始实验青壮年。 </li><li>建议开展“盲目”的检测。每个被检测的应变板应relabelled第二个实验者，使用的实验已经完成时，将东窗事发relabelled板进行检测。 </li><li>检测的一天，准备与4M原液的果糖，1％的刚果红溶液在室温下完全溶解。我...

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Neurexins和neuroligins执行突触传递的11和12个突触连接的分化中的重要作用。两种分子已被确定为自闭症13,14的候选基因。 在这个视频中，我们展示一个简单的方法，它可以让我们研究的影响渗透在C回避反应的基因的作用线虫 。的neuroligin缺陷的突变体缺陷检测渗透强度，但缺乏突变体在neurexin显示野生型菌株类似的响应，避免一个4M的果糖?...

表1。C.线虫株。 <table border="1" cellspacing="0" cellpadding="0"><tbody><tr><td> 应变 </td><td> 基因 </td><td> 等位基因 </td><td> 来源 </td></tr><tr><td>布里斯托尔氮气</td><td> - </td><td> - </td><td> ACGC </td></tr><tr><td> VC228 </td><td> NLG - 1 </td><td> ok259 </td><td>政府总部大楼</td></tr><tr><td> FX00474 </td><td> NLG - 1 </td><td> tm474 </td><td> bNBP - JAPAN </td></tr><tr><td> VC1416 </td><td> NRX - 1 </td><td> ok1649 </td><td>政府总部大楼</td></tr><tr><td> FX1961 </td><td> NRX - 1 </td><td> tm1961 </td><td> NBP - JAPAN </td></tr><tr><td> NL2099 </td><td> RRF - 3 </td><td> pk1436 </td><td>政府总部大楼</td></tr><tr><td> CRR21 </td><td> NRX - 1 NLG - 1; </td><td> ok1649; ok259 </td><td>这项工作</td></tr><tr><td> CRR22 </td><td> NRX - 1 NLG - 1; </td><td> tm1961; ok259 </td><td>这项工作</td></tr></tbody></table> A. 线虫遗传中心，美国明尼苏达大学。 B.国家实验动物“的线虫C. elegans的 ”生物资源项目。 日本东京女子医科大学。

osmotic avoidance in caenorhabditis elegans: synaptic function of two genes, orthologues of human nrxn1 and nlgn1, as candidates for autism

Neurexins和neuroligins目前在兴奋和抑制性突触细胞粘附分子，他们需要正确的神经网络功能 1 。这些蛋白在突触前和突触后膜2。在小鼠的研究表明，neurexins和neurologins在突触传递 1中的重要作用。最近的报告表明，改变神经元连接在人体神经系统的的发展可能构成的自闭症谱系障碍的许多案件3的病因的基础上。 线虫可以用来作为实验工具，方便的突触组成部分的运作的研究，因为它简单实验室试验，并得到了充分的特点，其神经系统和突触接线。 在 C 线虫NRX - 1和NLG - 1基因是同源NRXN1和NLGN1人类基因编码α- neurexin - 1和的neuroligin - 1蛋白，分别的。在人类与线虫，组织neurexins和neuroligins是类似的功能域。 线虫的头部包含amphid，线虫，介导的反应不同的刺激，包括渗透强度的感觉器官。 amphid 12纤毛树突和突触前终端轴突4感官双极神经元。这些神经元命名ASHR和ASHL，两个特别重要的渗透的感觉功能，检测与高渗透压实力 5水溶性的驱虫剂。这两种神经元的树突延长到嘴的尖端和轴突延伸到神经环，在那里他们作出决定的行为反应 6的其他神经元突触连接。 为了评估在高渗透压的力量避免neurexin和的neuroligin的影响，我们展示了不同的反应NRX - 1和NLG - 1基因缺陷的线虫突变体，使用一个4M的果糖环7一种方法。行为表型被证实使用特定的RNAi 克隆 8 。 在 C 线虫 ，触发的RNAi的dsRNA可管理喂养9。通过食物传递的dsRNA诱导的利益，从而使遗传组件和网络途径的识别基因的RNAi干扰。

Watch this Scientific Journal Video about Osmotic Avoidance in Caenorhabditis elegans: Synaptic Function of Two Genes, Orthologues of Human NRXN1 and NLGN1, as Candidates for Autism at JoVE.com

Osmotic Avoidance in Caenorhabditis elegans: Synaptic Function of Two Genes, Orthologues of Human NRXN1 and NLGN1, as Candidates for Autism

Neurexins和neuroligins执行突触分化和传输中的重要作用的神经元膜的粘附蛋白。缺乏突变体的neuroligin<em&gt; C。线虫</em&gt;检测渗透强度的缺陷，但它们也包含在编码neurexin基因的突变时，他们恢复野生型表型。

渗透避免IN线虫：突触功能的两个基因，人类同源 NRXN1 NLGN1，作为候选人自闭症

Neurexins and neuroligins are cell adhesion molecules present in excitatory and inhibitory synapses, and they are required for correct neuron network ...

Research

JoVE Journal

Biology

Osmotic Avoidance in Caenorhabditis elegans: Synaptic Function of Two Genes, Orthologues of Human NRXN1 and NLGN1, as Candidates for Autism

Isolation and In vitro Activation of Caenorhabditis elegans Sperm

Isolation and In vitro Activation of Caenorhabditis elegans Sperm

分离和<em在体外激活线虫精子

Males and hermaphrodites are the two naturally found sexual forms in the nematode C. elegans. The amoeboid sperm are produced by both males and ...

科研

生物学

Time-lapse Microscopy of Early Embryogenesis in Caenorhabditis elegans

Time-lapse Microscopy of Early Embryogenesis in Caenorhabditis elegans

时间推移显微镜早期胚胎发育线虫

Caenorhabditis elegans has often been used as a model system in studies of early developmental processes.  The transparency of the 
embryos, the genetic ...

Basic Caenorhabditis elegans Methods: Synchronization and Observation

Basic Caenorhabditis elegans Methods: Synchronization and Observation

基本的<em&gt;线虫</em&gt;方法：同步和观察

Research into the molecular and developmental biology of the nematode Caenorhabditis elegans was begun in the early seventies by Sydney Brenner and it has ...

Biochemical and High Throughput Microscopic Assessment of Fat Mass in Caenorhabditis Elegans

Biochemical and High Throughput Microscopic Assessment of Fat Mass in Caenorhabditis Elegans

生化和高通量的微观评估的脂肪含量<em&gt;秀丽隐杆线虫</em

The nematode C. elegans has emerged as an important  model for the study of conserved genetic pathways regulating fat metabolism as  it relates to human ...

Prostaglandin Extraction and Analysis in Caenorhabditis elegans

Prostaglandin Extraction and Analysis in Caenorhabditis elegans

前列腺素的提取与分析<em&gt;线虫</em

Caenorhabditis elegans is emerging as a powerful animal model to study the biology of lipids1-9. Prostaglandins are an important class of eicosanoids, ...

Using Caenorhabditis elegans as a Model System to Study Protein Homeostasis in a Multicellular Organism

Using Caenorhabditis elegans as a Model System to Study Protein Homeostasis in a Multicellular Organism

The folding and assembly of proteins is essential for protein function, the long-term health of the cell, and longevity of the organism. Historically, the ...

Dietary Supplementation of Polyunsaturated Fatty Acids in Caenorhabditis elegans

Dietary Supplementation of Polyunsaturated Fatty Acids in Caenorhabditis elegans

多不饱和脂肪酸的膳食补充剂<em&gt;秀丽隐杆线虫</em

Fatty acids are essential for numerous cellular functions. They serve as efficient energy storage molecules, make up the hydrophobic core of membranes, ...

Sample Preparation and Imaging of Exosomes by Transmission Electron Microscopy

透射电镜下体的样品制备与成像

Exosomes are nano-sized extracellular vesicles secreted by body fluids and are known to represent the characteristics of cells that secrete them. The ...

Measurement of Oxygen Consumption Rates in Intact Caenorhabditis elegans

Measurement of Oxygen Consumption Rates in Intact Caenorhabditis elegans

线虫的耗氧率测定

Optimal mitochondrial function is critical for healthy cellular activity, particularly in cells that have high energy demands like those in the nervous ...

Modeling Age-Associated Neurodegenerative Diseases in Caenorhabditis elegans

Modeling Age-Associated Neurodegenerative Diseases in Caenorhabditis elegans

建模年龄相关的神经退行性疾病在 卡诺哈布迪蒂斯埃利根

Battling human neurodegenerative pathologies and managing their pervasive socioeconomic impact is becoming a global priority. Notwithstanding their ...