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DOI :
10.3791/162-v
February 25th, 2007
Chapters
2:57
Assemble hanging drop culture
6:05
Starting EB culture
0:06
Assembling handing drops differentiation culture
15:18
Transplantation
11:25
Preparation of ES cell-derived progenitors for FACS
0:08
Collect undifferentiated ES cells
16:19
Discussion
2:24
Prepare materials
4:34
Collecting differentiated ES cells form hanging drops
6:53
EB dissociation and infection
このプロトコルの詳細マウス胚性幹細胞(ESC)と致死量の放射線照射、レシピエントマウスへの彼らのその後の注射から移植造血幹細胞の派生。簡単に言うと、ESCは、レトロウイルスHoxB4とOP9ストローマ細胞と造血サイトカインとの共培養に感染している胚様体、として区別されます。
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