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DOI :
10.3791/1662-v
•
12:55 min
January 7th, 2010
Chapters
0:00
Title
0:44
Introduction
2:07
Preparing the Mouse Embryonic Fibroblasts
4:00
The 96-well Electroporation Plates
5:20
Electroporating the MEFs in 96-well Plates
10:11
Epifluorescence Microscopy Images of Transfected Cells
12:26
Conclusion
7:49
Electroporating the MEFs in Cuvettes
此过程说明如何使用基因脉冲MXcell电系统,以迅速和容易地识别小鼠胚胎成纤维细胞(MEFs)或其他主要细胞的最佳电条件。故障排除注意事项还讨论了相关的视频。
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