Name: live imaging of drosophila melanogaster embryonic hemocyte migrations
Uploaded: 2010-02-12T18:30:00
Description: Watch this Scientific Journal Video about Live Imaging Of Drosophila melanogaster Embryonic Hemocyte Migrations at JoVE.com

इस प्रोटोकॉल के भीतर और पॉल मार्टिन और एंटोनियो Jacinto की प्रयोगशालाओं के साथ सहयोग में हमारे काम के माध्यम से विकसित किया गया है. हम अपनी उत्कृष्ट सेवा के लिए और शेयर के लिए उड़ान भरने लाइनों जारी रखने के लिए ड्रोसोफिला समुदाय ब्लूमिंगटन स्टॉक केंद्र धन्यवाद. बी एस वर्तमान में एक बीबीएसआरसी परियोजना अनुदान द्वारा वित्त पोषित है. WW वेलकम ट्रस्ट कैरियर विकास फैलोशिप द्वारा वित्त पोषित है.

तैयार <ol><li> उपयुक्त ड्रोसोफिला एक hemocyte विशेष Gal4 (जैसे 2 SRP-Gal4) ड्राइवर और एक आनुवंशिक रूप से इनकोडिंग यूएएस नियंत्रण (जैसे UAS GFP) के तहत फ्लोरोसेंट संवाददाता युक्त लाइनों प्राप्त करते हैं . एसआरप...

<ol>
	<li>Brand, A. H., Perrimon, N. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Targeted+gene+expression+as+a+means+of+altering+cell+fates+and+generating+dominant+phenotypes.">Targeted gene expression as a means of altering cell fates and generating dominant phenotypes.</a> Development. 118, 401-415 (1993).</li><li>Bruckner, K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+PDGF/VEGF+receptor+controls+blood+cell+survival+in+Drosophila.">The PDGF/VEGF receptor controls blood cell survival in Drosophila.</a> Dev Cell. 7, 73-84 (2004).</li><li>Dutta, D., Bloor, J. W., Ruiz-Gomez, M., VijayRaghavan, K., Kiehart, D. 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S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=New+fluorescent+protein+reporters+for+use+with+the+Drosophila+Gal4+expression+system+and+for+vital+detection+of+balancer+chromosomes.">New fluorescent protein reporters for use with the Drosophila Gal4 expression system and for vital detection of balancer chromosomes.</a> Genesis. 34, 135-138 (2002).</li><li>Sullivan, W., Ashburner, M., Hawley, R. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> Drosophila protocols. , (2000).</li><li>Tepass, U., Fessler, L. 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इस प्रक्रिया का सबसे महत्वपूर्ण तत्वों को स्पष्ट रूप से लेबल hemocytes के साथ स्वस्थ भ्रूण के चयन कर रहे हैं और उन्हें नुकसान पहुँचाए बिना उन्हें ध्यान से माउंट. एक बार भ्रूण हेलोकार्बन तेल में हैं वे निर्जल...

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live imaging of drosophila melanogaster embryonic hemocyte migrations

कई अध्ययनों से पता सेल का उपयोग प्रवास<em&gt; इन विट्रो में</em&gt; विधियों, जबकि physiologically प्रासंगिक पर्यावरण कि जीव ही है. यहाँ हम बढ़ते के लिए एक प्रोटोकॉल वर्तमान<em&gt; ड्रोसोफिला मेलानोगास्टर</em&gt; भ्रूण और fluorescently लेबल hemocytes के बाद रहते इमेजिंग, इस जीव के भ्रूण मैक्रोफेज. Gal4 - UAS प्रणाली का उपयोग<sup&gt; 1</sup&gt; हम आनुवंशिक रूप से इनकोडिंग hemocytes में fluorescently टैग मार्करों भ्रूण भर में अपने विकास प्रसार का पालन करें की एक किस्म की अभिव्यक्ति ड्राइव. भ्रूण के विकास के वांछित स्तर पर निम्नलिखित संग्रह, बाहरी chorion निकाल दिया है और भ्रूण तो एक hydrophobic, गैस पारगम्य झिल्ली और रहते इमेजिंग के लिए एक गिलास coverslip के बीच हेलोकार्बन तेल में बढ़ रहे हैं. गति और दिशात्मकता, उच्च संकल्प एफ actin और सूक्ष्मनलिकाएं अधिक इन cytoskeletal घटकों की गतिशीलता से संबंधित विस्तृत जानकारी प्रदान कर सकते हैं फ्लोरोसेंट संवाददाताओं के उपयोग के साथ युग्मित इमेजिंग के रूप में सकल प्रवासी मापदंडों के अलावा.

Watch this Scientific Journal Video about Live Imaging Of Drosophila melanogaster Embryonic Hemocyte Migrations at JoVE.com

Live Imaging Of Drosophila melanogaster Embryonic Hemocyte Migrations

ड्रोसोफिला hemocytes विकासशील भ्रूण की सम्पूर्णता पर फैलाने. इस प्रोटोकॉल को दर्शाता है कैसे माउंट करने के लिए और छवि इन fluorescently लेबल hemocytes के साथ भ्रूण का उपयोग प्रवास.

के लाइव इमेजिंग ड्रोसोफिला मेलानोगास्टर भ्रूण Hemocyte migrations

Many studies address cell migration using in vitro methods, whereas the physiologically relevant environment is that of the organism itself. Here we ...

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Live Imaging Of Drosophila melanogaster Embryonic Hemocyte Migrations

Dissection of Larval CNS in Drosophila Melanogaster

ड्रोसोफिला मेलानोगास्टर में लारवल सीएनएस के विच्छेदन

The central nervous system (CNS) of Drosophila larvae is complex and poorly understood.  One way to investigate the CNS is to use immunohistochemistry to ...

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Confocal माइक्रोस्कोपी द्वारा Zebrafish भ्रूण मस्तिष्क की इमेजिंग लाइव

In this video, we demonstrate the method our lab has developed to analyze the cell shape changes and rearrangements required to bend and fold the ...

Live-cell Imaging and Quantitative Analysis of Embryonic Epithelial Cells in Xenopus laevis

Live-cell Imaging and Quantitative Analysis of Embryonic Epithelial Cells in Xenopus laevis

लाइव सेल इमेजिंग और भ्रूणीय उपकला कोशिकाओं के मात्रात्मक विश्लेषण में Xenopus laevis</em

Embryonic epithelial cells serve as an ideal model to study morphogenesis where multi-cellular tissues undergo changes in their geometry, such as changes ...

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In Drosophila melanogaster, as in other insects, a waxy layer on the outer surface of the cuticle, composed primarily of hydrocarbon compounds, provides ...

Isolation of Drosophila melanogaster Testes

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The testes of Drosophila melanogaster provide an important model for the study of stem cell maintenance and differentiation, meiosis, and soma-germline ...

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Live cell imaging is an important technique applied to a number of Drosophila tissues used as models to investigate topics such as axis specification, ...

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में प्रोटीन GFP लेबल की इमेजिंग लाइव<em&gt; ड्रोसोफिला</em&gt; Oocytes

The  Drosophila oocyte has been established as a versatile system for investigating  fundamental questions such as cytoskeletal function, cell ...

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उम्र के मापन में<em&gt; ड्रोसोफिला मेलानोगास्टर</em

Aging is a phenomenon that results in steady physiological deterioration in nearly all organisms in which it has been examined, leading to reduced ...

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Apoptotic सेल क्लीयरेंस की इमेजिंग के दौरान जीते<em&gt; ड्रोसोफिला</em&gt; Embryogenesis

The  proper elimination of unwanted or aberrant cells through apoptosis and  subsequent phagocytosis (apoptotic cell clearance) is crucial for normal  ...

Imaging Through the Pupal Case of Drosophila melanogaster

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की पोटा संबंधी मामले के माध्यम से इमेजिंग<em&gt; ड्रोसोफिला मेलानोगास्टर</em

The longstanding use of Drosophila as a model for cell and developmental biology has yielded an array of tools. Together, these techniques have enabled ...