Name: a reversible, non-invasive method for airway resistance measurements and bronchoalveolar lavage fluid sampling in mice
Uploaded: 2010-04-13T17:00:00
Description: Watch this Scientific Journal Video about A Reversible, Non-invasive Method for Airway Resistance Measurements and Bronchoalveolar Lavage Fluid Sampling in Mice at JoVE.com

我们感谢W。Mintzer博士的建议，执行纤维气管插管。支持的赠款U19AI070973，R01AI057696，K02HL75243，并从美国国立卫生研究院R01HL082487。

过敏原的挑战： <ol><li> C57BL / 6小鼠，年龄4-8周，是在一个密闭的有机玻璃清除氧蒸汽混合物在3.2％异氟醚10分钟，以实现深全身麻醉室麻醉。 </li><li>鼻腔过敏原的挑战（45μLOVA（22.5微克）和7μLA.水稻（7微克），在PBS）的管理，每星期二，星期四和星期日，共连续七年申请。 </li></ol>麻醉： <ol start="3"><li>管理之前，每个过敏原的挑战，并按照第七挑战，小鼠腹腔注射48毫克/公?...

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	<li>Hamelmann, E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Noninvasive+measurement+of+airway+responsiveness+in+allergic+mice+using+barometric+plethysmography.">Noninvasive measurement of airway responsiveness in allergic mice using barometric plethysmography.</a> Am. J. Respir. Crit. Care Med. 156, 766-775 (1997).</li><li>Adler, A., Cieslewicz, G., Irvin, C. G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Unrestrained+plethysmography+is+an+unreliable+measure+of+airway+responsiveness+in+BALB/c+and+C57BL/6+mice.">Unrestrained plethysmography is an unreliable measure of airway responsiveness in BALB/c and C57BL/6 mice.</a> J. Appl. Physiol. 97, 286-292 (2004).</li><li>Bates, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+use+and+misuse+of+penh+in+animal+models+of+lung+disease.">The use and misuse of penh in animal models of lung disease.</a> Am. J. Respir. Cell Mol. Biol. 31, 373-374 (2004).</li><li>Lundblad, L. K., Irvin, C. G., Adler, A., Bates, J. H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+reevaluation+of+the+validity+of+unrestrained+plethysmography+in+mice.">A reevaluation of the validity of unrestrained plethysmography in mice.</a> J. Appl. Physiol. 93, 1198-1207 (2002).</li><li>Grunig, G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Requirement+for+IL-13+independently+of+IL-4+in+experimental+asthma.">Requirement for IL-13 independently of IL-4 in experimental asthma.</a> Science. 282, 2261-2263 (1998).</li><li>Corry, D. B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Requirements+for+allergen-induced+airway+hyperreactivity+in+T+and+B+cell-deficient+mice.">Requirements for allergen-induced airway hyperreactivity in T and B cell-deficient mice.</a> Mol. Med. 4, 344-355 (1998).</li><li>Corry, D. B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Interleukin+4,+but+not+interleukin+5+or+eosinophils,+is+required+in+a+murine+model+of+acute+airway+hyperreactivity.">Interleukin 4, but not interleukin 5 or eosinophils, is required in a murine model of acute airway hyperreactivity.</a> J. Exp. Med. 183, 109-117 (1996).</li><li>Amdur, M. O., Mead, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Mechanics+of+respiration+in+unanesthetized+guinea+pigs.">Mechanics of respiration in unanesthetized guinea pigs.</a> Am J Physiol. 192, 364-368 (1958).</li></ol>

哮喘的研究，以及其他各种气道阻塞性疾病，构成一个生物医学研究的活跃和扩大的领域。与哮喘有关的实验研究的一个重要组成部分，是衡量不同条件下的气道大小的变化的能力。过多的气道狭窄回应挑衅性的挑战，哮喘和相关的肺部疾病的一个典型特征，称为气道高反应性呼吸道财产，是一个有临床意义的攻击导致气短，呼吸困难等症状，其中包括死亡的重要组成部分。 <p class="jove_content...

呼吸道生理测量软件（Rescomp）是自定义的准备（千年总理集团; 415-519-4371）。 使用一台PC运行Windows XP的工作站，配备Pentium III的CPU（英特尔）公司，美国加州圣克拉拉，和一个17针的模拟数字信号转换器（美国国家仪器，＃PC - LPM16）的数据进行分析。 小动物的呼吸道生理工作站自定义组装（千年总理集团）使用市售压力传感器（＃TRD5700和TRD4510），前置放大器模块（部件编号：MAX2270），底盘（部分＃MAX1320; Buxco，北卡罗莱纳州威尔明顿）和一个定制的小动物体积描记。 0.5毫米螺纹外径光纤连接到光源（科尔帕尔默照明灯，41722系列） 呼吸机（美国哈佛大学器械鼠标风机，＃687） 10毫米，27ga针（BD公司，猫没有。309602） 散热灯 1 ml注射器（BD公司，猫没有。305109） 4个夹子（小马3200弹簧夹） 0.5毫米的外部插管导丝 Hemacytometer Superfrost / PLUS显微镜载玻片（费舍尔猫。12-550-15） Shandon过滤卡（热猫没有。5991022） 差分细胞幻灯片染色（费舍尔猫。。122911） 光显微镜（Leica） Cytospin 3（Shandon） 20 GA，1.25英寸ProtectIV（史密斯医疗）静脉导管 0.5毫米聚合物光纤（埃德蒙光学＃NT02 - 532）。

a reversible, non-invasive method for airway resistance measurements and bronchoalveolar lavage fluid sampling in mice

实验性哮喘模型气道高反应（AHR）的测量和支气管肺泡灌洗（BAL）流体取样是必不可少的，但重复的程序，以获取这样的测量，在相同的动物一般都是不可行的。在这里，我们展示了用于从小鼠中获得的AHR的反复测量和支气管肺泡灌洗液标本的协议。小鼠超过14天的挑战滴鼻，一个强有力的过敏原或治疗深水七倍。之前最初的挑战，并在24小时内，每个鼻内挑战，相同的动物麻醉后，经口头插管和机械通气。 AHR，通过增加乙酰胆碱（Ach）深水和过敏原的挑战动物之间的氯化的静脉注射引起的呼吸道系统阻力（RRS）的剂量反应曲线比较评估，进行了测定。之后，通过相同的插管，左肺灌洗，使气道细胞的差枚举可以执行。这些研究显示，反复测量，AHR的BAL液收集可能来自同一动物，在7-10天开始过敏原的挑战实现，最大的气道高反应性气道嗜酸性粒细胞增多。这种新颖的技术，大大减少了纵向实验所需的老鼠的数量，并适用于各种啮齿类动物，疾病模型气道生理仪器。

Watch this Scientific Journal Video about A Reversible, Non-invasive Method for Airway Resistance Measurements and Bronchoalveolar Lavage Fluid Sampling in Mice at JoVE.com

A Reversible, Non-invasive Method for Airway Resistance Measurements and Bronchoalveolar Lavage Fluid Sampling in Mice

重复测量的啮齿类动物的呼吸生理学和呼吸道炎症细胞取样是可取的，但一般并不可行。在这里，我们描述了一种可重复的方法，口头插管的小鼠气道高反应和呼吸道炎症细胞取样的反复测量，允许。

一个可逆的，非侵入性方法，气道阻力测量和支气管肺泡灌洗小鼠流体取样

Airway hyperreactivity (AHR) measurements and bronchoalveolar lavage (BAL) fluid sampling are essential to experimental asthma models, but repeated ...

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