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DOI :
10.3791/1726-v
February 3rd, 2010
Chapters
0:00
Title
0:47
Introduction
1:18
Assembling Injection Slides and Imaging Slides
2:36
Injecting DNA Constructs
4:43
Mounting Embryos for Imaging
6:52
4D Imaging of Cell Behaviors on Olympus FV1000 Confocal with IX81 Microscope
8:49
Behavior of Individual Labeled Neurons and Neural Crest Cells
10:23
Conclusion
このプロトコルは、ゼブラフィッシュ胚の生体内で個々の神経細胞や神経堤細胞のイメージングを説明します。このメソッドは、蛍光共焦点タイムラプス顕微鏡を使用して細胞の行動とアクチンの局在を調べるために使用されます。
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