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DOI :
10.3791/1726-v
February 3rd, 2010
Chapters
0:00
Title
0:47
Introduction
1:18
Assembling Injection Slides and Imaging Slides
2:36
Injecting DNA Constructs
4:43
Mounting Embryos for Imaging
6:52
4D Imaging of Cell Behaviors on Olympus FV1000 Confocal with IX81 Microscope
8:49
Behavior of Individual Labeled Neurons and Neural Crest Cells
10:23
Conclusion
이 프로토콜은 zebrafish 배아를 생활에서 개별 뉴런 또는 신경 능선 세포의 이미징을 설명합니다. 이 방법은 형광 공촛점 시간 경과 현미경을 사용하여 셀룰러 행동과 굴지의 현지화을 검사하는 데 사용됩니다.
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