Name: synthesis and calibration of phosphorescent nanoprobes for oxygen imaging in biological systems
Uploaded: 2010-03-03T17:30:00
Description: Watch this Scientific Journal Video about Synthesis and Calibration of Phosphorescent Nanoprobes for Oxygen Imaging in Biological Systems at JoVE.com

来自美国国家卫生研究院的资助EB007279和HL081273的支持表示感谢。

1。氧气测量协议的一般描述 （本节不会有任何行动，但对于理解本文的其余部分的关键。例如，它可以被拍摄下来，，作为一个序列数的Power Point幻灯片，伴随着的声音。） 1.1）探头传递到利益的媒介，例如，在一个动物的血液或组织间液注射。 1.2）的对象（组织表面）是适当波长的光照亮，以促进其激发三重态的探测。通常情况...

<ol>
	<li>Vanderkooi, J. M., Maniara, G., Green, T. J., Wilson, D. F. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=An+optical+method+for+measurement+of+dioxygen+concentration+based+on+quenching+of+phosphorescence.">An optical method for measurement of dioxygen concentration based on quenching of phosphorescence.</a> J. Biol. Chem. 262, 5476-5482 (1987).</li><li>Rumsey, W. L., Vanderkooi, J. M., Wilson, D. F. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Imaging+of+phosphorescence:+A+novel+method+for+measuring+the+distribution+of+oxygen+in+perfused+tissue.">Imaging of phosphorescence: A novel method for measuring the distribution of oxygen in perfused tissue.</a> Science. 241, 1649-1651 (1988).</li><li>Lebedev, A. Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Dendritic+phosphorescent+probes+for+oxygen+Imaging+in+biological+systems.">Dendritic phosphorescent probes for oxygen Imaging in biological systems.</a> Acs Applied Materials and Interfaces. 1, 1292-1304 (2009).</li><li>Finikova, O. S., Cheprakov, A. V., Beletskaya, I. P., Carroll, P. J., Vinogradov, S. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Novel+versatile+synthesis+of+substituted+tetrabenzoporphyrins.">Novel versatile synthesis of substituted tetrabenzoporphyrins.</a> Journal of Organic Chemistry. 69, 522-535 (2004).</li><li>Lindsey, J. S., Schreiman, I. C., Hsu, H. C., Kearney, P. C., Marguerettaz, A. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Rothemund+and+Adler-Longo+Reactions+revisited:+Synthesis+of+tetraphenylporphyrins+under+equilibrium+conditions.">Rothemund and Adler-Longo Reactions revisited: Synthesis of tetraphenylporphyrins under equilibrium conditions.</a> Journal of Organic Chemistry. 52, 827-836 (1987).</li><li>Lebedev, A. Y., Troxler, T., Vinogradov, S. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Design+of+metalloporphyrin-based+dendritic+nanoprobes+for+two-photon+microscopy+of+oxygen.">Design of metalloporphyrin-based dendritic nanoprobes for two-photon microscopy of oxygen.</a> J. Porphyrins and Phthalocyanines. 12, 1261-1269 (2008).</li></ol>

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synthesis and calibration of phosphorescent nanoprobes for oxygen imaging in biological systems

氧气测量磷光猝灭[1，2]包括以下步骤：1）探头被交付到利益的媒介（如血液或组织间液）; 2）对象是适当波长的光照射，以激发探测到它的三重态; 3）所发出的磷光是收集，分析其时间当然是产生的磷光寿命，这是转换成氧气的浓度（或分压，PO<sub&gt; 2</sub&gt;）。探头必须与生物环境相互作用，并在某些情况下4）从媒介测量完成后排出体外。上述每个步骤规定上的磷光探针，从而构成侵入组件的唯一的测量协议的分子设计的要求。在这里，我们审查为在生物系统中的氧气测量的树突状磷光纳米传感器的设计。探头由铂或钯卟啉的polyarylglycine（AG）树枝状，改性聚乙二醇（PEG）残留外围。对于有效的双光子激发，总站的树枝状双光子天线生色，捕获的激发能量通过分子内和通道探头的三重核心的FRET（福斯特共振能量转移）可能会被修改。我们描述的光物理性质的探头和目前详细的校正协议的关键。

Watch this Scientific Journal Video about Synthesis and Calibration of Phosphorescent Nanoprobes for Oxygen Imaging in Biological Systems at JoVE.com

Synthesis and Calibration of Phosphorescent Nanoprobes for Oxygen Imaging in Biological Systems

我们目前磷光淬火和审查设计基于卟啉 - 树突状纳米传感器在生物系统中的氧气成像的氧气测量的原则。

氧成像在生物系统中的磷光纳米探针的合成和校准

Oxygen measurement by phosphorescence quenching [1, 2] consists of the following steps: 1) the probe is delivered into the medium of interest (e.g. blood ...

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