Name: ambulatory ecg recording in mice
Uploaded: 2010-05-27T17:00:00
Description: Watch this Scientific Journal Video about Ambulatory ECG Recording in Mice at JoVE.com

马克麦考利是支持由美国国立卫生研究院辅导的培训补助金5T32HL066991 - 07 Xander的Wehrens是支持由美国国立卫生研究院/ NHLBI补助1R01HL091947 01A209和3R01HL089598 - 03S109

1。遥测手术植入的制备<ol><li>插入鼠标心电图遥测之前，重要的是要确保遥测心电图是无菌的，在良好的工作秩序。通常是在无菌条件下由制造商提供新的遥测设备。可重复使用心电图遥测提供的设备是使用至少4个小时Tergazyme 1％溶液清洗。您可以用无菌水冲洗Tergazyme清洗后的遥测。此外，使用Wavicide - 01的消毒剂一夜之间消毒心电图遥测。请务必用无菌水冲洗消毒液植入到小鼠体内，48小时前?...

<ol>
	<li>Cerrone, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Bidirectional+ventricular+tachycardia+and+fibrillation+elicited+in+a+knock-in+mouse+model+carrier+of+a+mutation+in+the+cardiac+ryanodine+receptor.">Bidirectional ventricular tachycardia and fibrillation elicited in a knock-in mouse model carrier of a mutation in the cardiac ryanodine receptor.</a> Circ Res. 96 (10), e77-e77 (2005).</li><li>Chelu, M. G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Calmodulin+kinase+II-mediated+sarcoplasmic+reticulum+Ca2++leak+promotes+atrial+fibrillation+in+mice.">Calmodulin kinase II-mediated sarcoplasmic reticulum Ca2+ leak promotes atrial fibrillation in mice.</a> J Clin Invest. 119 (7), 1940-1940 (1940).</li><li>Sood, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Intracellular+calcium+leak+due+to+FKBP12.6+deficiency+in+mice+facilitates+the+inducibility+of+atrial+fibrillation.">Intracellular calcium leak due to FKBP12.6 deficiency in mice facilitates the inducibility of atrial fibrillation.</a> Heart Rhythm. 5 (7), 1047-1047 (2008).</li><li>Swoap, S. J., Gutilla, M. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Cardiovascular+changes+during+daily+torpor+in+the+laboratory+mouse.">Cardiovascular changes during daily torpor in the laboratory mouse.</a> Am J Physiol Regul Integr Comp Physiol. 297 (3), R769-R769 (2009).</li><li>Costa-Goncalves, d. a., C, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Role+of+the+multidomain+protein+spinophilin+in+blood+pressure+and+cardiac+function+regulation.">Role of the multidomain protein spinophilin in blood pressure and cardiac function regulation.</a> Hypertension. 52 (4), 702-702 (2008).</li><li>Wehrens, X. H., Kirchhoff, S., Doevendans, P. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Mouse+electrocardiography:+an+interval+of+thirty+years.">Mouse electrocardiography: an interval of thirty years.</a> Cardiovasc Res. 45 (1), 231-231 (2000).</li></ol>

此过程的关键步骤是正确的清洗和小鼠生存手术前的准备。当遥测和鼠标手术部位被正确地清理，有一个更好的手术后的结果。此外，密切关注导致安置，减少手术后的皮肤糜烂。可能修改的技术包括替代的配置，以评估横向左心室壁功能（导致我的配置），或使用不仅为遥测心电波形分析，也超出了本文的范围温度，甚至血压分析[] 。心电遥测记录的一个替代的位置是皮下导致外部导线的位置?...

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ambulatory ecg recording in mice

在小鼠体内的遥测心电图记录是必不可少的了解背后的机制性心律失常，传导障碍，心脏猝死。虽然体表心电图是利用短期测量波形的时间间隔，它是不实际的心率变异或捕获罕见的心律失常发作的长期研究。植入式心电遥测提供简单的植入手术，长期卧床小鼠电图的记录，并与多种动物的同步录音的可扩展性的优势。在这里，我们提出了动态心电图记录在小鼠植入遥测一步一步的指导。认真遵守无菌技术操作是必需的植入和记录的可能性，从几周到几个月的有利的生存结果。因此，植入式心电遥测是一个有价值的关键信息在日间的动物模型，如鼠标心脏电生理检测工具。

Watch this Scientific Journal Video about Ambulatory ECG Recording in Mice at JoVE.com

Ambulatory ECG Recording in Mice

遥测心电图已成为一个必不可少的工具在评估心律失常和心脏猝死动物模型。在这里，我们提出了一个在长期的动态心电图监测在小鼠中的应用遥测心电图录音，逐步引导。

在小鼠体内的动态心电图记录

Telemetric ECG recording in mice is essential to understanding the mechanisms behind arrhythmias, conduction disorders, and sudden cardiac death. Although ...

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