Name: determining the reactivity and titre of serum using a haemagglutination assay
Uploaded: 2010-01-29T00:00:00
Description: Watch this Scientific Journal Video about Determining the Reactivity and Titre of Serum using a Haemagglutination Assay at JoVE.com

<p class="jove_content">笔者想感谢劳拉马修斯小姐表演的技巧和摄影郑明牧羊犬。这个项目是由南澳大学，实验室医学程序。 </p>

<p class="jove_title">制备的5倍Veronal缓冲液（VBS） </p><ol><li>要准备Veronal缓冲液（VBS），3个单独的解决方案都需要做好准备。 </li><li>准备通过溶解在蒸馏水350毫升NaCl和0.94gm钠巴比妥21.25gm溶液1。氯化钠和钠巴比妥终浓度分别为1.02M和13MM。 </li><li>准备通过溶解在热蒸馏水125毫升巴比妥1.44gm溶液2。巴比妥最终浓度62.5mM。 </li><li>准备通过溶解在蒸馏水100ml <sub>氯化钙</sub>氯化镁<sub>2</sub>和4.41gm 20.33gm溶液3。 <sub>2</sub>氯化镁...

<p class="jove_content">血凝检测是一个非常简单的的技术，允许大量的血清样本，将在很短的时间内评估。它还具有无需要专门的设备读取的优势，与合适的眼睛。这种技术也被修改为检测尿中的人绒毛膜促性腺激素激素，作为一个血凝抑制试验的一部分。在有关标准的血凝试验，有一个需要注意的是为了正确执行检测的几个领域。首先，这项技术是基于一个凹面上的细胞沉淀。因此，至关重要的是，使用一个U型或圆...

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<td>Revercells</td>
<td>&nbsp;</td>
<td>CSL</td>
<td>02490201</td>
<td>Use at 1% final</td>
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<tr>
<td>Anti-A serum</td>
<td>&nbsp;</td>
<td>CSL</td>
<td>02611305</td>
<td>&nbsp;</td>
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<td>96 well U bottom plate</td>
<td>&nbsp;</td>
<td>TRP</td>
<td>92097</td>
<td>Must be U-bottom</td>
</tr>
<tr>
<td>Veronal buffered saline</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>Use at 1x final</td>
</tr>
<tr>
<td>37&deg;C room/incubator</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
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determining the reactivity and titre of serum using a haemagglutination assay

<p class="jove_content">血凝是一种特定的凝集的形式，使用时红血细胞，它作为颗粒性抗原抗体结合。红血细胞是特别有用的目标，因为他们都是现成的，是用肉眼观察凝集。这种技术常用来确定的抗体滴度（AB），血型和病毒定量。在这个视频，在编制和执行血凝检测所涉及的步骤演示使用一个抗原添加到红血细胞（Revercells）血型特异性抗体。抗血清稀释96以及U型底酶标托盘，这是增加了一个悬挂的Revercells的。样本混合在一起，然后在37 ° C孵育60分钟。过了这段时间，样品可以很容易地取得VE，+ ve和中间的(-/+)血凝反应。此方法允许使用一个快速和简单的的技术进行评估血清样品的活性和滴度。视频将覆盖检测背后的理论，如何理解和解释的结果是，如何确定滴度是可以修改，如何检测和使用这项技术与相关的任何问题。</p>

Watch this Scientific Journal Video about Determining the Reactivity and Titre of Serum using a Haemagglutination Assay at JoVE.com

Determining the Reactivity and Titre of Serum using a Haemagglutination Assay

<p class="jove_content">血凝是抗体结合到红细胞的凝集形成。红血细胞都是现成的，结果很容易用肉眼观察。本视频演示了在血凝实验所涉及的步骤，解释的结果，并确定滴度。</p>

确定使用血凝检测血清中的反应和滴度

Haemagglutination is a specific form of agglutination and is used when antibodies bind to red blood cells, which act as a particulate antigen. Red blood ...

Research

JoVE Journal

Immunology and Infection

Measuring the 50% Haemolytic Complement (CH<sub>50</sub>) Activity of Serum

Measuring the 50% Haemolytic Complement CH50 Activity of Serum

测量50％的溶血性补充（CH<sub> 50</sub>）活性的血清

The complement system is a group of proteins that when activated lead to target cell lysis and facilitates phagocytosis through opsonisation. Individual ...

科研

免疫与感染

Determining the Phagocytic Activity of Clinical Antibody Samples

确定临床抗体样品的吞噬活性

Antibody-driven phagocytosis is induced via the engagement of Fc receptors on professional phagocytes, and can contribute to both clearance as well as ...

Determining Optimal Cytotoxic Activity of Human Her2neu Specific CD8 T cells by Comparing the Cr51 Release Assay to the xCELLigence System

确定最佳人力Her2neu特异性CD8 + T细胞的细胞毒活性的比较CR51发行含量xCELLigence系统

Cytotoxic CD8 T cells constitute a subgroup of T  cells that are capable of inducing the death of infected or malignant host  cells1. These cells express ...

Investigating the Effects of Probiotics on Pneumococcal Colonization Using an <em>In Vitro</em> Adherence Assay

Investigating the Effects of Probiotics on Pneumococcal Colonization Using an In Vitro Adherence Assay

使用一个调查益生素对肺炎球菌定植的影响<em&gt;体外</em&gt;粘附试验

Adherence of Streptococcus pneumoniae (the pneumococcus) to the epithelial lining of the nasopharynx can result in colonization and is considered a ...

Pairwise Growth Competition Assay for Determining the Replication Fitness of Human Immunodeficiency Viruses

成对增长竞争测定法测定人类免疫缺陷病毒的复制健身

In vitro fitness assays are essential tools for determining viral replication fitness for viruses such as HIV-1. Various measurements have been used to ...

High-throughput Quantitative Real-time RT-PCR Assay for Determining Expression Profiles of Types I and III Interferon Subtypes

高通量实时定量RT-PCR检测方法的确定I型和III型干扰素亚型的表达谱

Described in this report is a qRT-PCR assay for the analysis of seventeen human IFN subtypes in a 384-well plate format that incorporates highly specific ...

Assessing Retinal Microglial Phagocytic Function <em>In Vivo</em> Using a Flow Cytometry-based Assay

Assessing Retinal Microglial Phagocytic Function In Vivo Using a Flow Cytometry-based Assay

评估视网膜小胶质细胞吞噬功能<em&gt;在体内</em&gt;使用基于流式细胞术流量测定

Microglia are the tissue resident macrophages of the central nervous system (CNS) and they perform a variety of functions that support CNS homeostasis, ...

Optimization of a Quantitative Micro-neutralization Assay

定量微中和试验的优化

The micro-neutralization (MN) assay is a standard technique for measuring the infectivity of the influenza virus and the inhibition of virus replication. ...

<em>In Vivo</em> Assay for Detection of Antigen-specific T-cell Cytolytic Function Using a Vaccination Model

In Vivo Assay for Detection of Antigen-specific T-cell Cytolytic Function Using a Vaccination Model

<em>在体内</em>应用免疫模型检测抗原特异性 T 细胞细胞功能的试验

Current methodologies for antigen-specific killing are limited to in vitro use or utilized in infectious disease models. However, there is not a protocol ...

A Simple Flow Cytometry Based Assay to Determine <em>In Vitro</em> Antibody Dependent Enhancement of Dengue Virus Using Zika Virus Convalescent Serum

A Simple Flow Cytometry Based Assay to Determine In Vitro Antibody Dependent Enhancement of Dengue Virus Using Zika Virus Convalescent Serum

基于流式细胞仪的 Zika 病毒疗养血清测定登革热病毒的<em>体外</em>抗体依赖性

Antibody dependent enhancement of infection has been shown to play a major role in Dengue viral pathogenesis. Traditional assays that measure the capacity ...