Name: a microfluidic device for quantifying bacterial chemotaxis in stable concentration gradients
Uploaded: 2010-04-19T17:40:00
Description: Watch this Scientific Journal Video about A Microfluidic Device for Quantifying Bacterial Chemotaxis in Stable Concentration Gradients at JoVE.com

这项工作是支持部分由美国国家科学基金会（CBET 0846453）。

1。制备硅的主人，使用标准的SU - 8光刻1（在这个视频没有显示）。 <ol><li>使用标准的SU - 8光刻方法，编造包含微流体网络和趋化室的PDMS模具的苏- 8“主”（SU - 8 2025年，MicroChem，牛顿，MA）的创建。这种掌握模具，可以制作任何微细加工设施（例如，斯坦福大学的微流体铸造<a href="http://thebigone.stanford.edu/foundry/">;</a> http://thebigone.stanford.edu/foundry/） ​​。 </li><li>的PDMS复制之前，?...

<ol>
	<li>McDonald, J. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Prototyping+of+microfluidic+devices+in+poly(dimethylsiloxane)+using+solid-object+printing.">Prototyping of microfluidic devices in poly(dimethylsiloxane) using solid-object printing.</a> Anal Chem. 74, 1537-1545 (2002).</li><li>Hansen, M. C., Palmer, R. J. J., Udsen, C., White, D. C., Molin, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Assessment+of+GFP+fluorescence+in+cells+of+Streptococcus+gordonii+under+conditions+of+low+pH+and+low+oxygen+concentration.">Assessment of GFP fluorescence in cells of Streptococcus gordonii under conditions of low pH and low oxygen concentration.</a> Microbiol. 147, 1383-1391 (2001).</li><li>Englert, D. L., Manson, M. D., Jayaraman, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+Flow-Based+Microfluidic+Device+for+Quantifying+Bacterial+Chemotaxis+in+Stable,+Competing+Gradients.">A Flow-Based Microfluidic Device for Quantifying Bacterial Chemotaxis in Stable, Competing Gradients.</a> Appl Environ Microbiol. 75, 4557-4564 (2009).</li><li>Englert, D. L., Jayaraman, A., Manson, M. D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> Microfluidic techniques for the analysis of bacterial chemotaxis. , (2009).</li><li>Mao, H., Cremer, P. S., &amp;amp, M. a. n. s. o. n. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+sensitive,+versatile+microfluidic+assay+for+bacterial+chemotaxis.">A sensitive, versatile microfluidic assay for bacterial chemotaxis.</a> PNAS. 100 (9), 5449-5454 (2003).</li></ol>

在毛泽东等人 5所描述的趋化分配系数（CPC）和趋化迁移系数（CMC），可以计算出。如果一个细胞上检测到高浓度侧，它是一个价值1，而在低浓度侧检测的单元格值-1。值总结和细胞总数除以产生中国共产党。中国共产党的符号（正或负）给出的迁移方向（朝向或远离信号）。虽然中国共产党表示是否细胞作为一种化学引诱或驱蚊剂，它没有量化的趋化程度。对于这一点，我们除?...

a microfluidic device for quantifying bacterial chemotaxis in stable concentration gradients

趋使细菌方法诱食化学品的来源，或避免驱蚊化学品的来源。细菌不断地监视当前浓度的浓度进行比较具体chemoeffectors的浓度检测早几秒钟。这种比较，决定运动的净方向。虽然多个，竞争梯度往往共存于大自然，调查细菌的趋化作用的常规方法是最理想量化引诱剂和驱虫的浓度梯度迁移。在这里，我们描述了一个微流体的趋化模型，提出chemoeffectors精确和稳定的浓度梯度细菌和定量调查应用梯度的响应的发展。该装置是在任何所需的绝对浓度和梯度强度，浓度梯度的多功能，可以很容易扩散混合产生。该设备是证明使用的响应<em&gt;大肠埃希氏菌</em&gt; RP437氨基酸和镍离子的梯度。

Watch this Scientific Journal Video about A Microfluidic Device for Quantifying Bacterial Chemotaxis in Stable Concentration Gradients at JoVE.com

A Microfluidic Device for Quantifying Bacterial Chemotaxis in Stable Concentration Gradients

本协议描述了一个微流体装置的发展，为细菌的趋化调查chemoeffectors稳定的浓度梯度。

一个微流体装置量化细菌趋稳定的浓度梯度

Chemotaxis allows bacteria to approach sources of attractant chemicals or to avoid sources of repellent chemicals. Bacteria constantly monitor the ...

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