Name: electrode fabrication and implantation in aplysia californica for multi-channel neural and muscular recordings in intact, freely behaving animals
Uploaded: 2010-06-04T17:00:00
Description: Watch this Scientific Journal Video about Electrode Fabrication and Implantation in Aplysia californica for Multi-channel Neural and Muscular Recordings in Intact, Freely Behaving Animals at JoVE.com

我们非常感谢来自美国国立卫生研究院（NS047073 HJC）的支持。我们也感谢凯瑟琳凯尔佳柏实，鼓励我们为我们的应用程序中使用它与她的早期实验。

1。电极的制作<ol><li>要创建的电极，用剪刀切了一块搪瓷涂层0.001英寸直径的不锈钢丝约2英尺长。每个线端连接一个小球的腻子，折了一半的线，和自旋创建一个双绞线的两端，称为差分电极。 </li><li>胶带两端的导线，以防止解开。四个差分电极，使用不同的颜色为每个用于识别目的的差分电极（图1A）的磁带。 </li><li>在另一端的差分电极一起离开两英寸，两个扭无捻一端和1英寸。重复其余?...

<ol>
	<li>Lu, H., Chestek, C. A., Shaw, K. M., Chiel, H. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Selective+extracellular+stimulation+of+individual+neurons+in+ganglia.">Selective extracellular stimulation of individual neurons in ganglia.</a> J. Neural Eng. 5, 287-309 (2008).</li><li>Morton, D. W., Chiel, H. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=In+vivo+buccal+nerve+activity+that+distinguishes+ingestion+from+rejection+can+be+used+to+predict+behavioral+transitions+in+Aplysia.">In vivo buccal nerve activity that distinguishes ingestion from rejection can be used to predict behavioral transitions in Aplysia.</a> J Comp. Physiol. A. 172, 17-32 (1993).</li><li>Warman, E. N., Chiel, H. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+new+technique+for+chronic+single+extracellular+recording+in+freely+behaving+animals+using+pipette+electrodes.">A new technique for chronic single extracellular recording in freely behaving animals using pipette electrodes.</a> J. Neurosci. Methods. 57, 161-169 (1995).</li><li>Ye, H., Morton, D. W., Chiel, H. J., J, H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=nbsp;Neuromechanics+of+multifunctionality+during+rejection+in+Aplysia+californica.">nbsp;Neuromechanics of multifunctionality during rejection in Aplysia californica.</a> J. Neurosci. 26, 10743-10755 (2006).</li></ol>

这些录音成功的一个关键的创新是结合成一个电缆的电极。已使用过的两个电极录音，而是扩大到四个独立的电极在手术后粘连的形成，往往无法正常进食运动动物。结合成一个电缆的电极，降低了大部分内的动物，从而降低免疫反应的电极，使动物更自由地移动。 此过程中的创新等方面的强力胶和佳- SIL的组合，连接电极的神经或肌肉。在此之前，只有强力胶使用，但两...

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electrode fabrication and implantation in aplysia californica for multi-channel neural and muscular recordings in intact, freely behaving animals

从主要神经和肌肉的录制<em&gt;海兔</em&gt;在摄食行为，让我们在一个完整的动物研究的神经控制模式。同时记录多个神经和肌肉，为我们提供了准确的信息有关的神经活动的时间。以前的记录方法有两个电极的工作，但更多的神经和肌肉的研究所需的多种动物的录音，这使得它难以确定的时机和逐步精细的细节，因为变性，合并和平均响应响应，并从动物动物。植入四个独立的电极，具有非常低的成功率，由于形成粘连，防止动物执行正常进食运动。我们开发新的方法，减少了大量的动物允许正常进食运动的内部电极的电极制造。使用胶水的组合，附加在一个更可靠的绝缘，持续时间较长的电极，电极的结果，使得它可以记录长达一个星期的时期。描述的制造技术，我们可以扩展到包含一些额外的电极，将适用于脊椎动物。

Watch this Scientific Journal Video about Electrode Fabrication and Implantation in Aplysia californica for Multi-channel Neural and Muscular Recordings in Intact, Freely Behaving Animals at JoVE.com

Electrode Fabrication and Implantation in Aplysia californica for Multi-channel Neural and Muscular Recordings in Intact, Freely Behaving Animals

一种技术是植入四个描述<em&gt;在体内</em&gt;电极监测摄食行为的神经肌肉控制<em&gt;海兔californica</em&gt;。

电极的制作及植入海兔californica完整的，自由的行为的动物多通道的神经和肌肉的录音

Recording from key nerves and muscles of Aplysia during feeding behavior allows us to study the patterns of neural control in an intact animal.  ...

Research

JoVE Journal

Neuroscience

Electrode Fabrication and Implantation in Aplysia californica for Multi-channel Neural and Muscular Recordings in Intact, Freely Behaving Animals

Combining Behavioral Endocrinology and Experimental Economics: Testosterone and Social Decision Making

睾酮和社会决策，结合行为内分泌和实验经济学：

Behavioral endocrinological research in humans as well as in animals suggests that testosterone plays a key role in social interactions. Studies in ...

科研

神经科学

High-density EEG Recordings of the Freely Moving Mice using Polyimide-based Microelectrode

高密度的自由移动，使用基于聚酰亚胺的微电极的小鼠的脑电图记录

Electroencephalogram (EEG) indicates the averaged electrical activity of the neuronal populations on a large-scale level. It is widely utilized as a ...

Design and Assembly of an Ultra-light Motorized Microdrive for Chronic Neural Recordings in Small Animals

设计和组装的超轻型电动微型硬盘（Microdrive）慢性神经录音的小动物

The ability to chronically record from populations of neurons in freely behaving animals has proven an invaluable tool for dissecting the function of ...

An In Vitro Preparation for Eliciting and Recording Feeding Motor Programs with Physiological Movements in Aplysia californica

An In Vitro Preparation for Eliciting and Recording Feeding Motor Programs with Physiological Movements in Aplysia californica

一个<em在体外</em制备与生理变动，为激发和记录进给电机程序<em&gt;海兔夜蛾</em

Multifunctionality, the ability of one peripheral  structure to generate multiple, distinct behaviors1, allows animals  to rapidly adapt their behaviors ...

Dual Electrophysiological Recordings of Synaptically-evoked Astroglial and Neuronal Responses in Acute Hippocampal Slices

双突触诱发的星形胶质细胞和神经元的反应，在急性海马脑片电生理记录

Astrocytes form together with neurons tripartite synapses, where they integrate and modulate neuronal activity. Indeed, astrocytes sense neuronal inputs ...

Extracellularly Identifying Motor Neurons for a Muscle Motor Pool in Aplysia californica

Extracellularly Identifying Motor Neurons for a Muscle Motor Pool in Aplysia californica

运动神经元细胞外识别的肌肉运动游泳池<em&gt;海兔夜蛾</em

In animals with large  identified neurons (e.g. mollusks), analysis of motor pools is done using  intracellular techniques1,2,3,4. Recently,  we developed ...

Isolation of Sensory Neurons of Aplysia californica for Patch Clamp Recordings of Glutamatergic Currents

Isolation of Sensory Neurons of Aplysia californica for Patch Clamp Recordings of Glutamatergic Currents

隔离的感觉神经元中<em&gt;海兔丫</em谷氨酸能电流膜片钳记录

The marine gastropod mollusk Aplysia californica has a venerable  history as a model of nervous system function, with particular significance in  studies ...

A Procedure for Implanting Organized Arrays of Microwires for Single-unit Recordings in Awake, Behaving Animals

一个程序植入微丝的有组织阵列的单机录音清醒，举止动物

In vivo electrophysiological recordings in the awake, behaving animal provide a powerful method for understanding neural signaling at the single-cell ...

Design and Fabrication of Ultralight Weight, Adjustable Multi-electrode Probes for Electrophysiological Recordings in Mice

设计和超轻重量的制作，可调节的多电极电生理记录小鼠探头

The number of physiological investigations in the mouse, mus musculus, has experienced a recent surge, paralleling the growth in methods of genetic ...

Visual Evoked Potential Recordings in Mice Using a Dry Non-invasive Multi-channel Scalp EEG Sensor

干无创多通道头皮脑电传感器在小鼠视觉诱发电位记录中的应用

For scalp EEG research environments with laboratory mice, we designed a dry-type 16 channel EEG sensor which is non-invasive, deformable, and re-usable ...