Name: isolation of human umbilical vein endothelial cells (huvec)
Uploaded: 2007-04-28T00:00:00
Description: Watch this Scientific Journal Video about Isolation of Human Umbilical Vein Endothelial Cells HUVEC at JoVE.com

<p class="jove_step">手順</p><ol><li>過剰な血液オフクリーンパッドとDABにコードを置く。コー​​ドの両端で新鮮なカットを行います。 </li><li>静脈に、ONプラスチック針のシースで<strong>21 1 / 2 Gの針を挿入します</strong> 。 （静脈の最大の開口部である、2小さいものが動脈です。） </li><li>止血剤で固定<strong>針をクランプ</strong>し、針にハンクスの20ccシリンジを取り付けます。 </li><li>適度な圧力で静脈<strong>を通し...

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<td>0.1% Collagenase </td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>10ml per cord, warmed to 37&#176;C</td>
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<td>Tissue culture flasks</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>T75, one per cord.</td>
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<td>Hanks medium</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
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<td>scissors</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>sterile</td>
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<td>beaker</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>sterile</td>
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<td>50 ml tubes</td>
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isolation of human umbilical vein endothelial cells (huvec)

<p class="jove_content">血管新生は血管新生の刺激に応答して、新しい血管が既存の血管系から作成される複雑なマルチステップのプロセスです。これらの手順は、次のとおりコード、管腔形成、吻合、および新たな基底膜の形成に細胞外マトリックス、ECの整列への内皮細胞の基底膜、増殖と移動（萌芽）（EC）の低下。 in vitroアッセイの多くは、このプロセスを研究するために開発が、ほとんど唯一の血管新生の特定の段階を模倣し、形態学的に血管が多くの場合、in vivoで血管似ていないされています。ここでは、ヒト臍帯静脈ECと線維芽細胞を利用したin vitroでの血管新生アッセイに最適化を示しています。このモデルは、反復する血管新生の重要な初期段階のすべてを、そして重要な血管が偏ECに囲まれた特許細胞間ルーメンを表示します。血管は容易に位相コントラストとタイムラプス顕微鏡で観察し、下流のアプリケーション用に純粋な形で回収することができます。 </p>

Watch this Scientific Journal Video about Isolation of Human Umbilical Vein Endothelial Cells HUVEC at JoVE.com

Isolation of Human Umbilical Vein Endothelial Cells HUVEC

<p class="jove_content">このビデオプロトコルは、ヒト臍帯からヒト臍帯静脈内皮細胞（HUVEC）の単離と文化を示しています。一旦単離さこれらの細胞はまた、ヒューズの研究室で実証最適化されたフィブリンゲルビーズアッセイなどのin vitroでの血管新生アッセイに使用することができます。</p>

ヒト臍帯静脈内皮細胞の単離（HUVEC）

Angiogenesis is a complex multi-step process, where in response to angiogenic stimuli, new vessels are created from the existing vasculature. These steps ...

Research

JoVE Journal

Biology

Isolation of Human Umbilical Vein Endothelial Cells (HUVEC)

Isolation and Large Scale Expansion of Adult Human Endothelial Colony Forming Progenitor Cells

分離と成人ヒト内皮コロニー形成前駆細胞の大規模拡張

This paper introduces a novel recovery strategy for endothelial colony forming progenitor cells (ECFCs) from heparinized but otherwise unmanipulated adult ...

Isolation and Animal Serum Free Expansion of Human Umbilical Cord Derived Mesenchymal Stromal Cells (MSCs) and Endothelial Colony Forming Progenitor Cells (ECFCs)

Isolation and Animal Serum Free Expansion of Human Umbilical Cord Derived Mesenchymal Stromal Cells MSCs and Endothelial Colony Forming Progenitor Cells ECFCs

ヒト臍帯由来間葉系幹細胞（MSCs）と血管内皮コロニー形成前駆細胞（ECFCs）の単離および動物血清自由膨張

The umbilical cord is a rich source for progenitor cells with high proliferative potential including mesenchymal stromal cells (also termed mesenchymal ...

Isolation of Human Umbilical Arterial Smooth Muscle Cells (HUASMC)

Isolation of Human Umbilical Arterial Smooth Muscle Cells HUASMC

ヒト臍帯動脈平滑筋細胞の単離（HUASMC）

The human umbilical cord (UC) is a biological sample that can be easily obtained just after birth. This biological sample is, most of the time, discarded ...

Isolation of Valvular Endothelial Cells

弁内皮細胞の単離

Heart valves are solely responsible for maintaining unidirectional blood flow through the cardiovascular system.  These thin, fibrous tissues are ...

Isolation and Culture of Pulmonary Endothelial Cells from Neonatal Mice

新生児マウスからの肺血管内皮細胞の単離と培養

Endothelial cells provide a useful research model in many areas of vascular biology. Since its first isolation 1, human umbilical vein endothelial cells ...

Phenotypic and Functional Characterization of Endothelial Colony Forming Cells Derived from Human Umbilical Cord Blood

ヒト臍帯血由来血管内皮コロニー形成細胞の表現型および機能解析

Longstanding views of new blood vessel formation via angiogenesis, vasculogenesis, and arteriogenesis have been recently reviewed1. The presence of ...

Isolation of Embryonic Ventricular Endothelial Cells

胚心室内皮細胞の単離

Cell culture has greatly enhanced our ability to assess individual populations of cells under myriad culture conditions. While immortalized cell lines ...

Isolation of Murine Valve Endothelial Cells

ネズミ弁内皮細胞の単離

Normal valve structures consist of stratified layers of specialized extracellular matrix (ECM) interspersed with valve interstitial cells (VICs) and ...

Isolation and Primary Culture of Mouse Aortic Endothelial Cells

マウス大動脈内皮細胞の単離および初代培養

The vascular endothelium is essential to normal vascular homeostasis. Its dysfunction participates in various cardiovascular disorders. The mouse is an ...

Isolation of Mouse Coronary Endothelial Cells

マウスの冠動脈内皮細胞の単離

Endothelial cells line the inner wall of blood vessels and play an important role in the regulation of vascular tone, vascular permeability, and new ...