Name: harvesting sperm and artificial insemination of mice
Uploaded: 2007-04-28T00:00:00
Description: Watch this Scientific Journal Video about Harvesting Sperm and Artificial Insemination of Mice at JoVE.com

<p>我想感谢迈克杜威从他宝贵的见解Peromyscus遗传股票中心，耐心，和啮齿类动物繁殖知识。 </p>

<p class="jove_title">人工授精</p><p class="jove_content">人工授精代替自然交配，以增加成功的机会，所有的女性会怀孕。用于每1超排的女性或在发情已知一名女2男。 caudae附睾成熟的雄性小鼠被放置在了9％的牛奶悬挂和切片18表针开放。精子被允许自由游泳几分钟，然后是附睾从混合物。悬架被评估为合流和活动精子的显微镜。钝的22号1 ½英寸长针是用来管理精子。 120 °弯曲放置约3 / 4 <sup>次</sup> ，上下针方式。弯曲和0...

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<html xmlns="http://www.w3.org/1999/xhtml" xml:lang="en" lang="en">	
		
		<div>
		<table border="1">
		<thead>
		<tr>
		<th>Material Name</th>
		<th>Type</th>
		<th>Company</th>
		<th>Catalogue Number</th>
		<th>Comment</th>
		</tr>
		</thead>
		<tbody>
		
<tr>
<td>Petri Dish</td>
<td>Tool</td>
<td>Fisher</td>
<td>08-772-30 </td>
<td>&nbsp;</td>
<tr>
<td>18 gauge needle</td>
<td>Tool</td>
<td>Becton Dickinson</td>
<td>305195</td>
<td>&nbsp;</td>
<tr>
<td>Powdered milk</td>
<td>Reagent</td>
<td>Any Grocery store</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<tr>
<td>1 ml syringe</td>
<td>Tool</td>
<td>National Scientific Company</td>
<td>S7510-1</td>
<td>&nbsp;</td>
<tr>
<td>Forceps</td>
<td>Tool</td>
<td>Roboz</td>
<td>RS-5060</td>
<td>&nbsp;</td>
<tr>
<td>Scissors</td>
<td>Tool</td>
<td>Roboz</td>
<td>RS-5882</td>
<td>&nbsp;</td>
<tr>
<td>Dulbecco's Phosphate Buffered Saline</td>
<td>Reagent</td>
<td>Gibco</td>
<td>14200-075</td>
<td>&nbsp;</td>
<tr>
<td>22 gauge blunt needle</td>
<td>Tool</td>
<td>Give to me</td>
<td>&nbsp;</td>
<td>&nbsp;</td>		</tbody>
		</table>
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harvesting sperm and artificial insemination of mice

<p class="jove_content">鼠类属Peromyscus（鹿鼠）是最普遍的原生北美的哺乳动物。 Peromyscus物种的研究范围广泛，包括毒物学，流行病学，生态，行为，和遗传研究。在这里，他们提供了一个有用的模型示范人工授精。 </p><p class="jove_content">此处显示的方法类似先前已被用于一些鹿小鼠研究，但没有详细的协议已经出版。在这里，我们展示了人工受精的基本方法。这种方法需要从啮齿类动物中提取的睾丸，然后隔离精子从附睾和输精管的。在排卵期，精子的成熟，现在在一个牛奶混合后，放置在女性生殖道。施肥是算作0天的胚胎发育的时间。胚胎可以在需要的时间点检索和操纵。 </p><p class="jove_content">人工授精可用于在各种啮齿类动物是至关重要的，或难以取得确切的胚胎时机。这种技术是重要的物种或品系（包括最Peromyscus）可能无法立即交配和/或交配是很难评估。此外，人工受精胚胎发育的确切时间，无论是在映射发展进步和/或转基因工作。因为施肥是保证，可用于动物数量减少。这种方法已经进一步Peromyscus系统至关重要，以及将有望使他人受益。 </p>

Watch this Scientific Journal Video about Harvesting Sperm and Artificial Insemination of Mice at JoVE.com

Harvesting Sperm and Artificial Insemination of Mice

<p class="jove_content">此协议表明，从男性的睾丸中提取精子，然后inseminating雌性小鼠的方法。此过程需要精确的时间是在发育的研究，以及转基因工作时非常有用。</p>

收获的小鼠精子和人工授精

Rodents of the genus Peromyscus (deer mice) are the most prevalent native North American mammals.  Peromyscus species are used in a wide range of research ...

Research

JoVE Journal

Biology

Preparation of Artificial Bilayers for Electrophysiology Experiments

电生理实验人工双层膜的制备

Planar lipid bilayers, also called artificial lipid bilayers, allow you to study ion-conducting channels in a well-defined environment. These bilayers can ...

科研

生物学

Harvesting and Preparing <em>Drosophila</em> Embryos for Electrophysiological Recording and Other Procedures

Harvesting and Preparing Drosophila Embryos for Electrophysiological Recording and Other Procedures

收获和准备<em>果蝇</em>胚胎的电生理记录和其他程序

Drosophila is a premier genetic model for the study of both embryonic development and functional neuroscience. Traditionally, these fields are quite ...

Isolation and <em>In vitro</em> Activation of <em>Caenorhabditis elegans</em> Sperm

Isolation and In vitro Activation of Caenorhabditis elegans Sperm

分离和<em在体外</em>激活<em>线虫</em>精子

Males and hermaphrodites are the two naturally found sexual forms in the nematode C. elegans. The amoeboid sperm are produced by both males and ...

Mouse Sperm Cryopreservation and Recovery using the I&middot;Cryo Kit

Mouse Sperm Cryopreservation and Recovery using the IÃƒâ€šÃ‚Â·Cryo Kit

精子冷冻保存和恢复使用I ·冷冻包

Thousands of new genetically modified (GM) strains of mice have been created since the advent of transgenesis and knockout technologies.  Many of these ...

Assessing Differences in Sperm Competitive Ability in <em>Drosophila</em>

Assessing Differences in Sperm Competitive Ability in Drosophila

评估精子竞争能力的差异<em&gt;果蝇</em

Competition among conspecific males for fertilizing the ova is one of the mechanisms of sexual selection, i.e. selection that operates on maximizing the ...

<em>In Vitro</em> Reconstitution of Light-harvesting Complexes of Plants and Green Algae

In Vitro Reconstitution of Light-harvesting Complexes of Plants and Green Algae

<em>在</em>植物和绿藻的捕光配合<em>体外</em>重组

In plants and green algae, light is captured by the light-harvesting complexes (LHCs), a family of integral membrane proteins that coordinate chlorophylls ...

Avian Semen Collection by Cloacal Massage and Isolation of DNA from Sperm

泄殖腔按摩与精子 DNA 分离法采集禽精

Collection of semen may be useful for a wide range of applications including studies involving sperm quality, sperm telomere dynamics, and epigenetics. ...

Fish Sperm Assessment Using Software and Cooling Devices

使用软件和冷却装置进行鱼类精子评估

For gamete quality evaluation, there are innovative, rapid, and quantitative techniques that can provide useful data for aquaculture. Computerized systems ...

Harvesting and Disaggregation: An Overlooked Step in Biofilm Methods Research

收获和分解：生物膜方法研究中被忽视的步骤

Biofilm methods consist of four distinct steps: growing the biofilm in a relevant model, treating the mature biofilm, harvesting the biofilm from the ...

Artificial Intelligence Approaches to Assessing Primary Cilia

评估原发性纤毛的人工智能方法

Cilia are microtubule based cellular appendages that function as signaling centers for a diversity of signaling pathways in many mammalian cell types. ...