Name: retrieval of mouse oocytes
Uploaded: 2007-04-28T00:00:00
Description: Watch this Scientific Journal Video about Retrieval of Mouse Oocytes at JoVE.com

<p>我想感谢他的帮助，耐心和知识迈克杜威Peromyscus遗传库存中心。 </p>

<p class="jove_title">胚胎/取卵</p><ol><li>必须从-20 °解冻胚胎/卵母细胞的检索至少两个小时前，KSOM放置在37 ° C <sub>共</sub> 2孵化器和FHM的。一个玻璃盘，其中包含三个凹浅水井是用来房子KSOM滴在孵化器。为了防止脱水，KSOM和菜都放置在有盖的容器，在底部的水。该仪器可以防止不使用矿物油的蒸发。 <br /><br /></li><li>从排卵期女性输卵管被放置在FHM媒体。要确保的方向和一个完整的infindibulum，一个卵巢的一小部分被切...

<p>在这篇文章中，我们展示了从排卵诱导（超排）Peromyscus的卵母细胞的检索。利用超数排卵（相对于自然发情周期）是常见的，由于导致数字更大。然而，必须采取谨慎解释结果，利用这样的卵母细胞/胚胎，激素用于诱导排卵和培养可能有特效。 </p><p>当发情已证实，女性euthenized和输卵管与卵巢的一小部分一起回收。卵巢小区域被用作infindibulum的位置的标记。 infindibulum坐在旁边卵巢，经常通过组织一层薄薄的预?...

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		<div>
		<table border="1">
		<thead>
		<tr>
		<th>Material Name</th>
		<th>Type</th>
		<th>Company</th>
		<th>Catalogue Number</th>
		<th>Comment</th>
		</tr>
		</thead>
		<tbody>
		
<tr>
<td>Flushing needle</td>
<td>Tool</td>
<td>Zephyrtronics</td>
<td>ZT5-130-L</td>
<td>30 gauge blunt</td>
</tr>
<tr>
<td>Forceps (2)</td>
<td>Tool</td>
<td>Roboz</td>
<td>RS-5060</td>
<td>&nbsp;</td>
<tr>
<td>Peromyscus</td>
<td>Animal</td>
<td>Peromyscus Genetic Stock Center</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<tr>
<td>Scissors</td>
<td>Tool</td>
<td>Roboz</td>
<td>RS-5880</td>
<td>&nbsp;</td>
<tr>
<td>FHM HEPES Buffered Media</td>
<td>Reagent</td>
<td>Specialty Media</td>
<td>MR-025-d</td>
<td>&nbsp;</td>
<tr>
<td>KSOM Embryo Culture Medium (5x10 ml)</td>
<td>Reagent</td>
<td>Specialty Media</td>
<td>MR-020P-5F</td>
<td>&nbsp;</td>
<tr>
<td>Mouth Pipette</td>
<td>Tool</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>Pulled from glass capillaries and attached to rubber tubing</td>
</tr>
<tr>
<td>Oocyte holding dish</td>
<td>Tool</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>Glass container with concave divets</td>
</tr>		</tbody>
		</table>
		</div>
	

retrieval of mouse oocytes

<p class="jove_content">迄今为止，只有少数的研究报告Peromyscus胚胎发育或生殖生物学的成功操作。连同Peromyscus遗传联合中心（http://stkctr.biol.sc.edu），我们需要制定本系统的显着差异特征。一个主要目标，以优化的卵母细胞/早期胚胎的检索。</p>

Watch this Scientific Journal Video about Retrieval of Mouse Oocytes at JoVE.com

Retrieval of Mouse Oocytes

<p class="jove_content">该协议说明了从小鼠的输卵管中提取卵母细胞或早期胚胎受精技术。能够识别infindibulum和钝年底针插入正确执行的程序是必不可少的。</p>

检索小鼠卵母细胞

To date, only a few studies have reported successful manipulations of Peromyscus embryogenesis or reproductive biology.  Together with the Peromyscus ...

Research

JoVE Journal

Biology

Nuclear Transfer into Mouse Oocytes

小鼠卵母细胞的细胞核移植到

Nuclear transfer into an unfertilized oocyte can restore developmental potential to a differentiated cell. This demonstrates that the processes underlying ...

科研

生物学

Patch Clamp Recording of Ion Channels Expressed in  Xenopus Oocytes

补丁钳爪蟾卵母细胞中表达的离子通道

Since its development by Sakmann and Neher 1, 2, the patch clamp has become established as an extremely useful technique for electrophysiological ...

Microinjection of Xenopus Laevis Oocytes

非洲爪蟾卵母细胞的微量注射

Microinjection of Xenopus laevis oocytes followed by thin-sectioning electron microscopy (EM) is an excellent system for studying nucleocytoplasmic ...

Collection and Cryopreservation of Hamster Oocytes and Mouse Embryos

收集和仓鼠卵母细胞和小鼠胚胎冷冻保存

Embryos and oocytes were first successfully cryopreserved more than 30 years ago, when Whittingham et al. 1 and Wilmut 2 separately described that mouse ...

Primer Extension Capture: Targeted Sequence Retrieval from Heavily Degraded DNA Sources

引物延伸捕获：从严重退化的DNA来源有针对性的顺序检索

We present a method of targeted DNA sequence retrieval from DNA sources which are heavily degraded and contaminated with microbial DNA, as is typical of ...

Visualizing RNA Localization in <em>Xenopus</em> Oocytes

Visualizing RNA Localization in Xenopus Oocytes

可视化的RNA中的本地化<em>爪蟾</em>卵母细胞

RNA localization is a conserved mechanism of establishing cell polarity.  Vg1 mRNA localizes to the vegetal pole of Xenopus laevis oocytes and acts to ...

Fertilization of <em>Xenopus oocytes</em> using the Host Transfer Method

Fertilization of Xenopus oocytes using the Host Transfer Method

施肥<em>爪蟾卵母细胞</em>使用主机传输方法

Studying the contribution of maternally inherited molecules to vertebrate early development is often hampered by the time and expense necessary to ...

<em>In vivo</em> Imaging and Therapeutic Treatments in an Orthotopic Mouse Model of Ovarian Cancer

In vivo Imaging and Therapeutic Treatments in an Orthotopic Mouse Model of Ovarian Cancer

<em>活体</em>成像和治疗卵巢癌的原位小鼠模型

Human cancer and response to therapy is better represented in orthotopic animal models.  This paper describes the development of an orthotopic mouse model ...

Patch Clamp and Perfusion Techniques for Studying Ion Channels Expressed in <em>Xenopus</em> oocytes

Patch Clamp and Perfusion Techniques for Studying Ion Channels Expressed in Xenopus oocytes

膜片钳和灌注技术研究离子通道的表达<em>爪蟾</em>卵母细胞

The protocol presented here is designed to study the activation of the large conductance, voltage- and Ca2+-activated K+ (BK) channels. The protocol may ...

Generation of Mice Derived from Induced Pluripotent Stem Cells

诱导多能干细胞来源于小鼠的生成

The production of induced pluripotent stem cells (iPSCs) from somatic cells provides a means to create valuable tools for basic research and may also ...