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DOI :
10.3791/1852-v
March 22nd, 2010
Chapters
0:00
Title
0:47
Introduction
1:18
Isolation of Rat MSCs
2:40
Enrichment of Rat MSCs
5:17
Separation of Single-colony Derived MSCs
6:36
Results of Isolation and Enrichment of Rat MSCs
7:41
Conclusion
大鼠MSCs从股骨和胫骨分离,然后通过磁性细胞分选富集。排序细胞被证实为流式细胞仪检测表面标志物表达。这些细胞克隆密度培养,形成单菌落,然后这些菌落克隆气瓶分开。
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