Name: a model of disturbed flow-induced atherosclerosis in mouse carotid artery by partial ligation and a simple method of rna isolation from carotid endothelium
Uploaded: 2010-06-22T17:30:00
Description: Watch this Scientific Journal Video about A Model of Disturbed Flow-Induced Atherosclerosis in Mouse Carotid Artery by Partial Ligation and a Simple Method of RNA Isolation from Carotid Endothelium at

<p class="jove_content">这项工作是由美国国立卫生研究院拨款HL87012“（HJ），HL75209”（HJ），UO1HL80711（DH，DG HJ）和世界一流大学项目“（HJ）的资金从韩国的科学，技术和教育部的支持。 </p>

<p class="jove_title"> 1。部分结扎左侧颈内动脉</p><ol><li> 〜8周龄小鼠。建议至少18克的老鼠的重量。 </li><li> 30秒珠仪器灭菌消毒文书，并允许降温</li><li>诱导麻醉与氯胺酮（80mg/kg）和甲苯噻嗪（按10mg/kg）腹腔（IP）注射或替代麻醉IACUC批准。腹腔注射，注入鼠标在左侧腹部低象限。 </li><li>将鼠标放置在变暖，直至完全麻醉室。确认与脚趾捏的麻醉深度。 </li><li>将鼠标在手术阶段，在仰卧（腹朝上）位置。磁带脱颖而...

<ol>
	<li>Korshunov, V. A., Berk, B. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Flow-induced+vascular+remodeling+in+the+mouse:+a+model+for+carotid+intima-media+thickening.">Flow-induced vascular remodeling in the mouse: a model for carotid intima-media thickening.</a> <em style='font-style: italic'>Arterioscler Thromb Vasc Biol</em>. <b>23</b> (12), 2185-2191 (2003).</li><li>Sullivan, C. J., Hoying, J. B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Flow-dependent+remodeling+in+the+carotid+artery+of+fibroblast+growth+factor-2+knockout+mice.">Flow-dependent remodeling in the carotid artery of fibroblast growth factor-2 knockout mice.</a> <em style='font-style: italic'>Arterioscler Thromb Vasc Biol</em>. <b>22</b> (7), 1100-1105 (2002).</li><li>Nam, D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Partial+carotid+ligation+is+a+model+of+acutely+induced+disturbed+flow,+leading+to+rapid+endothelial+dysfunction+and+atherosclerosis.">Partial carotid ligation is a model of acutely induced disturbed flow, leading to rapid endothelial dysfunction and atherosclerosis.</a> <em style='font-style: italic'>Am J Physiol Heart Circ Physiol</em>. <b>297</b> (4), H1535-H1543 (2009).</li></ol>

<p class="jove_content">在此过程中，适当的识别所有常见的颈动脉分支，包括颈外动脉，颈内动脉，枕动脉，甲状腺上动脉是必不可少的。同样重要的是，以验证使用超声作为证明的流动模式。在我们经验丰富的的手中，我们的部分结扎手术的成功率超过500小鼠的超声研究为基础，超过90％。对于颈动脉内膜RNA的准备工作是很重要要注意的颈内动脉后出现的第一灌注步骤。流在这一点上左侧颈总动脉堵塞，如果有证据?...

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<td>Insulin syringe (3/10 ml 29g syringe)</td>
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a model of disturbed flow-induced atherosclerosis in mouse carotid artery by partial ligation and a simple method of rna isolation from carotid endothelium

<p class="jove_content">尽管众所周知的密切联系，不安流连接到动脉粥样硬化的直接证据，一直缺乏。最近，我们使用修改后的版本的颈动脉部分结扎方法[1,2]表明它敏锐地诱导低，振荡流的条件下，在小鼠左颈总动脉不安流的两个关键特征。使用这个模型，我们提供了直接证据，扰乱流量确实在载脂蛋白E基因敲除小鼠动脉粥样硬化的发展快速，稳健的[3]。我们还开发了高纯度的内皮细胞RNA制备方法，从小鼠颈动脉内膜[3]。利用这种小鼠模型和方法，我们发现，部分结扎血管内皮功能障碍，导致在一个星期稳健，快速的在两个星期的动脉粥样硬化的形成在高脂血症小鼠模型以及复杂的病变，如intraplaque新生血管形成的功能，四个星期。这种快速<em&gt;在体内</em&gt;模型和内皮细胞的RNA制备方法可以用来确定底层流依赖调节血管生物学和疾病的分子机制。此外，它可用于测试各种治疗干预，针对血管内皮功能障碍和动脉粥样硬化研究的时间大大减少。</p>

Watch this Scientific Journal Video about A Model of Disturbed Flow-Induced Atherosclerosis in Mouse Carotid Artery by Partial Ligation and a Simple Method of RNA Isolation from Carotid Endothelium at

A Model of Disturbed Flow-Induced Atherosclerosis in Mouse Carotid Artery by Partial Ligation and a Simple Method of RNA Isolation from Carotid Endothelium

<p class="jove_content">这描述了一个局部的颈动脉结扎手术，这会导致不安的水流条件和随后的动脉粥样硬化的发展（在两个星期）intraplaque新生血管在小鼠左颈总动脉（在四个星期内）。我们还描述了一个新颖的RNA从颈动脉内膜隔离方法，提供高纯度的内皮细胞的RNA。</p>

一个型号的不安流致动脉粥样硬化小鼠颈动脉部分结扎术和颈动脉内皮细胞的RNA分离的简单方法

Despite the well-known close association, direct evidence linking disturbed flow to atherogenesis has been lacking. We have recently used a modified ...

Research

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Implantation of a Carotid Cuff for Triggering Shear-stress Induced Atherosclerosis in Mice

植入小鼠触发剪应力致动脉粥样硬化的颈袖

It is widely accepted that alterations in vascular shear stress trigger the expression of inflammatory genes in endothelial cells and thereby induce ...

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Neointimal hyperplasia is one the primary causes of stenosis in arterialized veins that are of great importance in arterial coronary bypass surgery, in ...

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Despite  the considerable progress made in the stent development in the last decades,  cardiovascular diseases remain the main cause of death in western ...

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一个简单的方法小鼠肺插管

A  simple procedure to intubate mice for pulmonary function measurements would  have several advantages in longitudinal studies with limited numbers or  ...

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成年小鼠静脉高压型号：颈总动脉，以颈外静脉吻合。

The understanding of the pathophysiology of brain arteriovenous malformations and arteriovenous fistulas has improved thanks to animal models. A rat model ...

Carotid Artery Infusions for Pharmacokinetic and Pharmacodynamic Analysis of Taxanes in Mice

颈动脉输液的紫杉烷类化合物在小鼠体内的药代动力学和药效学分析

When proposing the use of a drug, drug combination, or drug delivery into a novel system, one must assess the pharmacokinetics of the drug in the study ...

Vascular Balloon Injury and Intraluminal Administration in Rat Carotid Artery

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The carotid artery balloon injury model in rats has been well established for over two decades. It remains an important method to study the molecular and ...

Ferric Chloride-induced Thrombosis Mouse Model on Carotid Artery and Mesentery Vessel

三氯化铁诱发血栓形成小鼠模型上的颈动脉和肠系膜血管

Severe thrombosis and its ischemic consequences such as myocardial infarction, pulmonary embolism and stroke are major worldwide health issues. The ferric ...

<em>In Vivo</em> Gene Transfer to the Rabbit Common Carotid Artery Endothelium

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<em>体内</em>兔颈总动脉内皮的基因转移

The goal of this method is to introduce a transgene into the endothelium of isolated segments of both rabbit common carotid arteries. The method achieves ...

A Rabbit Model of Durable Transgene Expression in Jugular Vein to Common Carotid Artery Interposition Grafts

颈动脉介入移植中颈静脉持久性转基因表达的家兔模型

Vein graft bypass surgery is a common treatment for occlusive arterial disease; however, long-term success is limited by graft failure due to thrombosis, ...