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DOI :
10.3791/1865-v
March 30th, 2010
Chapters
0:00
Title
0:37
Introduction
1:10
Designing a Yeast Expression System for Type III Effectors
4:21
Preparing Yeast Protein Extracts to Verify Effector Experession
2:13
Yeast Transformation
6:46
Spotting Assay to Detect Effector-induced Growth Inhibition Phenotypes
8:08
Spotting Assay Results
9:02
Conclusion
이 비디오에서는, 우리는 효모와 효과기 유발 성장 저해 phenotypes의 식별에 세균성 유형 III의 effectors의 표현을위한 절차를 설명합니다. 이러한 phenotypes는 이후 이펙터 기능과 목표를 명료하게하다에 악용될 수 있습니다.
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