Name: plastic embedding and sectioning of xenopus laevis embryos
Uploaded: 2007-04-29T00:00:00
Description: Watch this Scientific Journal Video about Plastic Embedding and Sectioning of Xenopus laevis Embryos at JoVE.com

固定<ol><li> MEMFAまたは小さなガラスシンチレーションバイアルの3.7％FA + PBS（フィッシャー猫＃03 - 339 - 25B）に全胚または切除した外植片を移す。それ以上だけでなく、2時間、室温でnutatorにバイアルをインキュベートする、と。 2時間以上のためのFAに胚を放置しないでください。 FAでより長いインキュベーションは、胚組織が難しくなると検出プロセス中の抗体の貧しい浸透に?...

あなたの抗原またはエピトープタグに対する一次抗体：PBTの適切な希釈を作る。ビオチン標識二次抗体：様々なベンダーは、ビオチン標識抗体を販売しています。

plastic embedding and sectioning of xenopus laevis embryos

Watch this Scientific Journal Video about Plastic Embedding and Sectioning of Xenopus laevis Embryos at JoVE.com

Plastic Embedding and Sectioning of Xenopus laevis Embryos

プラスチック製のセクションでは、組織の多くの種類のパラフィン包埋や凍結切片より、この方法は、より有用に、蛍光二次抗体で免疫染色することができる組織の薄切片における真の組織形態を維持する。染色、プラスチック製の埋め込み、およびセクショニングするための方法はこのビデオで示されている。

プラスチック製の埋め込みとアフリカツメガエル胚のセクショニング

Research

JoVE Journal

Biology

Dissection of Organizer and Animal Pole Explants from Xenopus laevis Embryos and Assembly of a Cell Adhesion Assay

アフリカツメガエルの胚および細胞接着アッセイのアセンブリから主催し、動物極の外植片の解剖

Obtaining Eggs from Xenopus laevis Females

アフリカツメガエル女性から卵子を入手

The eggs of Xenopus laevis intact, lysed, and/or fractionated are useful for a wide variety of experiments. This protocol shows how to induce egg laying, ...

Preparation and Fractionation of Xenopus laevis Egg Extracts

アフリカツメガエル卵抽出物の調製と分画

Crude and fractionated Xenopus egg extracts can be used to provide ingredients for reconstituting cellular processes for morphological and biochemical ...

Microinjection of Xenopus Laevis Oocytes

アフリカツメガエル卵母細胞のマイクロインジェクション

Microinjection of Xenopus laevis oocytes followed by thin-sectioning electron microscopy (EM) is an excellent system for studying nucleocytoplasmic ...

Tissue Determination Using the Animal Cap Transplant (ACT) Assay in Xenopus laevis

Tissue Determination Using the Animal Cap Transplant ACT Assay in Xenopus laevis

でアニマルキャップの移植（ACT）アッセイを用いて組織の決定アフリカツメガエル</em

Many proteins play a dual role in embryonic development. Those that regulate cell fate determination in a specific tissue can also affect the development ...

Live-cell Imaging and Quantitative Analysis of Embryonic Epithelial Cells in Xenopus laevis

Live-cell Imaging and Quantitative Analysis of Embryonic Epithelial Cells in Xenopus laevis

生細胞イメージングとの胚上皮細胞の定量分析アフリカツメガエル</em

Embryonic epithelial cells serve as an ideal model to study morphogenesis where multi-cellular tissues undergo changes in their geometry, such as changes ...

Production of Transgenic Xenopus laevis by Restriction Enzyme Mediated Integration and Nuclear Transplantation

Production of Transgenic Xenopus laevis by Restriction Enzyme Mediated Integration and Nuclear Transplantation

トランスジェニックの生産アフリカツメガエル制限酵素を介した統合と核移植によって

Stable integration of cloned gene products into the Xenopus genome is necessary to control the time and place of expression, to express genes at later ...

Facial Transplants in Xenopus laevis Embryos

Facial Transplants in Xenopus laevis Embryos

顔面移植<em&gt;アフリカツメガエル</em&gt;胚

Craniofacial birth defects occur in 1 out of every 700 live births, but etiology is rarely known due to limited understanding of craniofacial development. ...

Dissection of Xenopus laevis Neural Crest for in vitro Explant Culture or in vivo Transplantation

Dissection of Xenopus laevis Neural Crest for in vitro Explant Culture or in vivo Transplantation

の解剖<em&gt;アフリカツメガエル</em&gt;神経堤のため<em&gt; in vitroで</em&gt;外植片培養又は<em&gt; in vivoで</em&gt;移植

The neural crest (NC) is a transient dorsal neural tube cell population that undergoes an epithelium-to-mesenchyme transition (EMT) at the end of ...

Using plusTipTracker Software to Measure Microtubule Dynamics in Xenopus laevis Growth Cones

Using plusTipTracker Software to Measure Microtubule Dynamics in Xenopus laevis Growth Cones

で微小管ダイナミクスを測定するためにplusTipTrackerソフトウェアを使用した<em&gt;アフリカツメガエル</em&gt;成長円錐

Microtubule (MT) plus-end-tracking proteins (+TIPs) localize to the growing plus-ends of MTs and regulate MT dynamics1,2. One of the most well-known and ...