Name: plastic embedding and sectioning of xenopus laevis embryos
Uploaded: 2007-04-29T00:00:00
Description: Watch this Scientific Journal Video about Plastic Embedding and Sectioning of Xenopus laevis Embryos at JoVE.com

Fixering <ol><li> Överför hela embryon eller dissekeras explants i MEMFA eller 3,7% FA + PBS i ett litet glas scintillation injektionsflaska (Fisher katt # 03 till 339-25B). Inkubera flaskan på nutator vid RT, endast för 2 timmar och inte mer än så. Lämna inte embryo i FA för mer än 2 timmar. Längre inkubation i FA kommer att göra embryonal vävnad hårdare och resultera i en dålig penetration av antikroppar i identifieringsprocessen. </li><li> Aspirera flesta av FA och lägga D...

Primär antikropp mot din antigen eller taggar epitop: Gör en lämplig utspädning i PBT. Biotin-konjugerade sekundära antikroppar: olika leverantörer sälja biotin-konjugerade antikroppar.

plastic embedding and sectioning of xenopus laevis embryos

Watch this Scientific Journal Video about Plastic Embedding and Sectioning of Xenopus laevis Embryos at JoVE.com

Plastic Embedding and Sectioning of Xenopus laevis Embryos

Plast sektioner upprätthålla sant vävnad morfologi i tunna delar av vävnad som kan immunostained med fluorescerande sekundära antikroppar, vilket gör denna metod mer användbar än paraffininbäddade eller frysta snitt för många typer av vävnad. Metoden för färgning, plast inbäddning och snittning visas i denna video.

Plast inbäddning och Sektionering av Xenopus laevis embryon

Research

JoVE Journal

Biology

Dissection of Organizer and Animal Pole Explants from Xenopus laevis Embryos and Assembly of a Cell Adhesion Assay

Dissekering av Organizer och Animal explants Pole från Xenopus laevis Embryon och församlingen i en cell Adhesion analys

Obtaining Eggs from Xenopus laevis Females

Skaffa Ägg från Xenopus laevis Kvinnor

The eggs of Xenopus laevis intact, lysed, and/or fractionated are useful for a wide variety of experiments. This protocol shows how to induce egg laying, ...

Preparation and Fractionation of Xenopus laevis Egg Extracts

Upprättande och fraktionering av Xenopus laevis Egg Utdrag

Crude and fractionated Xenopus egg extracts can be used to provide ingredients for reconstituting cellular processes for morphological and biochemical ...

Microinjection of Xenopus Laevis Oocytes

Mikroinjektion av Xenopus laevis oocyter

Microinjection of Xenopus laevis oocytes followed by thin-sectioning electron microscopy (EM) is an excellent system for studying nucleocytoplasmic ...

Tissue Determination Using the Animal Cap Transplant (ACT) Assay in Xenopus laevis

Tissue Determination Using the Animal Cap Transplant ACT Assay in Xenopus laevis

Tissue Fastställande Använda Transplant Animal Cap (ACT) analys i Xenopus laevis</em

Many proteins play a dual role in embryonic development. Those that regulate cell fate determination in a specific tissue can also affect the development ...

Live-cell Imaging and Quantitative Analysis of Embryonic Epithelial Cells in Xenopus laevis

Live-cell Imaging and Quantitative Analysis of Embryonic Epithelial Cells in Xenopus laevis

Live-cell imaging och kvantitativ analys av embryonala epitelceller i Xenopus laevis</em

Embryonic epithelial cells serve as an ideal model to study morphogenesis where multi-cellular tissues undergo changes in their geometry, such as changes ...

Production of Transgenic Xenopus laevis by Restriction Enzyme Mediated Integration and Nuclear Transplantation

Production of Transgenic Xenopus laevis by Restriction Enzyme Mediated Integration and Nuclear Transplantation

Produktion av Transgena Xenopus laevis Genom begränsning enzymmedierad Integration och Nuclear Transplantation

Stable integration of cloned gene products into the Xenopus genome is necessary to control the time and place of expression, to express genes at later ...

Facial Transplants in Xenopus laevis Embryos

Facial Transplants in Xenopus laevis Embryos

Ansikts Transplantationer i<em&gt; Xenopus laevis</em&gt; Embryon

Craniofacial birth defects occur in 1 out of every 700 live births, but etiology is rarely known due to limited understanding of craniofacial development. ...

Dissection of Xenopus laevis Neural Crest for in vitro Explant Culture or in vivo Transplantation

Dissection of Xenopus laevis Neural Crest for in vitro Explant Culture or in vivo Transplantation

Dissekering av<em&gt; Xenopus laevis</em&gt; Neural Crest för<em&gt; In vitro</em&gt; Explantation kultur eller<em&gt; In vivo</em&gt; Transplantation

The neural crest (NC) is a transient dorsal neural tube cell population that undergoes an epithelium-to-mesenchyme transition (EMT) at the end of ...

Using plusTipTracker Software to Measure Microtubule Dynamics in Xenopus laevis Growth Cones

Using plusTipTracker Software to Measure Microtubule Dynamics in Xenopus laevis Growth Cones

Använda plusTipTracker Programvara för att mäta mikrotubuli Dynamics in<em&gt; Xenopus laevis</em&gt; Tillväxt Kottar

Microtubule (MT) plus-end-tracking proteins (+TIPs) localize to the growing plus-ends of MTs and regulate MT dynamics1,2. One of the most well-known and ...