Name: simultaneous fmri and electrophysiology in the rodent brain
Uploaded: 2010-08-19T17:00:00
Description: Watch this Scientific Journal Video about Simultaneous fMRI and Electrophysiology in the Rodent Brain at JoVE.com

这项工作是由美国国立卫生研究院支持1 R21NS057718 - 01。

1。这是一个非生存手术。第一步是植入电极。在这个例子中，将植入电极在两个半球的初级躯体感觉皮层的前爪地区。 <ol><li> 2％异氟醚麻醉大鼠（雄性SD大鼠，200-300克），立体定向手术系统修复到位。手术开始之前，确保动物麻醉，并没有一个脚趾头掐的响应展品。打开头皮之前删除的皮毛。上述颅骨的肌肉和其他组织分离，并阻止任何出血，骨表面使用cauterizer。 </li><li>码头准备在头骨表...

<ol>
	<li>Gruetter, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Automatic,+localized+in+vivo+adjustment+of+all+first-+and+second-order+shim+coils.">Automatic, localized in vivo adjustment of all first- and second-order shim coils.</a> Magn Reson Med. 29 (6), 804-804 (1993).</li><li>Logothetis, N. K., Pauls, J., Augath, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Neurophysiological+investigation+of+the+basis+of+the+fMRI+signal.">Neurophysiological investigation of the basis of the fMRI signal.</a> Nature. 412 (6843), 150-150 (2001).</li><li>Nir, Y., Fisch, L., Mukamel, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Coupling+between+neuronal+firing+rate,+gamma+LFP,+and+BOLD+fMRI+is+related+to+interneuronal+correlations.">Coupling between neuronal firing rate, gamma LFP, and BOLD fMRI is related to interneuronal correlations.</a> Curr Biol. 17 (15), 1275-1275 (2007).</li></ol>

电生理记录的BOLD功能磁共振成像技术分别发达。然而，同时录制和成像是具有挑战性，由于相互干扰的两 ​​种模式2。在这里，我们提供了一个可能的解决方案结合的啮齿类动物实验。电极植入的修改后的方法，最大限度地减少图像质量的影响，电气录音的工件去除是必要的，以消除噪声引起的图像采集。同时成像和记录在啮齿类动物中，将提供一个自发的神经活动和BOLD信号之间的耦?...

simultaneous fmri and electrophysiology in the rodent brain

要检查的血氧水平依赖（BOLD）的磁共振成像（MRI）信号的神经基础，我们已经开发出一种啮齿动物模型中，功能性磁共振成像数据，并<em&gt;在体内</em&gt; intracortical记录可以同时执行。 MRI和电气录音的结合是技术上具有挑战性，因为用于拍摄的电极扭曲的MRI图像和MRI的收购引起的电气录音中的噪音。为了尽量减少相互干扰的两种模式，分别用玻璃微电极，而不是金属和噪声去除算法实施的电生理数据。在我们的研究中，两个微电极分别植入双侧初级躯体感觉皮层（SI）的大鼠和固定到位。被选定为功能性磁共振成像冠状切片覆盖电极提示。电极轴和固定的位置不包括在图像切片，以避免成像伪影。去除头皮被替换用牙膏降低易感性不匹配，并防止图像吉布斯振铃文物。在快速转换的磁场在图像采集电气录音引起的工件结构特点，平均每个运行的扫描所有周期。成像过程中的噪音结构，然后从原始录音中扣除。降噪时间课程，然后用fMRI数据的进一步分析相结合。作为一个例子，同时采集用于确定自发的功能磁共振成像大胆的信号和带限intracortical电活动之间的关系。同时在啮齿类动物中的功能磁共振成像和电生理记录将在神经科学中的许多令人兴奋的应用，除了阐明的fMRI BOLD信号和神经元的活动之间的关系提供了一个平台。

Watch this Scientific Journal Video about Simultaneous fMRI and Electrophysiology in the Rodent Brain at JoVE.com

Simultaneous fMRI and Electrophysiology in the Rodent Brain

我们已经开发出一种方法同步功能磁共振成像和电生理记录在啮齿类动物的大脑的神经活动和血氧水平依赖（BOLD）的MRI信号之间的关系进行调查，提供了一个平台。

同时在啮齿动物脑功能磁共振成像和电

To examine the neural basis of the blood oxygenation level dependent (BOLD) magnetic resonance imaging (MRI) signal, we have developed a rodent model in ...

Research

JoVE Journal

Neuroscience

Design and Construction of a Cost Effective Headstage for Simultaneous Neural Stimulation and Recording in the Water Maze

同时神经刺激，并记录在水迷宫的设计和构建一个具有成本效益探头

Headstage preamplifiers and source followers are commonly used to study neural activity in behavioral neurophysiology experiments. Available commercial ...

科研

神经科学

Combining Transcranial Magnetic Stimulation and fMRI to Examine the Default Mode Network

结合经颅磁刺激和功能磁共振成像检查默认模式网络

The default mode network is a group of brain regions that are active when an individual is not focused on the outside world and the brain is at "wakeful ...

Co-analysis of Brain Structure and Function using fMRI and Diffusion-weighted Imaging

使用功能磁共振成像和弥散加权成像脑结构与功能分析

The study of complex computational systems is facilitated by network maps, such as circuit diagrams. Such mapping is particularly informative when ...

Simultaneous Electroencephalography, Real-time Measurement of Lactate Concentration and Optogenetic Manipulation of Neuronal Activity in the Rodent Cerebral Cortex

同时脑电图，实时测量的啮齿动物的大脑皮质中的神经元活动的乳酸浓度与Optogenetic的操作

Although the brain represents less than 5% of the body  by mass, it utilizes approximately one quarter of the glucose used by the body  at rest1. The ...

Deep Brain Stimulation with Simultaneous fMRI in Rodents

深部脑刺激与同步功能磁共振成像在鼠害

In order to visualize the global and downstream neuronal responses to deep brain stimulation (DBS) at various targets, we have developed a protocol for ...

In vivo Optogenetic Stimulation of the Rodent Central Nervous System

In vivo Optogenetic Stimulation of the Rodent Central Nervous System

在啮齿动物中枢神经系统的体内光遗传学刺激

The ability to probe defined neural circuits in awake, freely-moving animals with cell-type specificity, spatial precision, and high temporal resolution ...

Simultaneous Electrophysiological Recording and Micro-injections of Inhibitory Agents in the Rodent Brain

同时电生理记录和抑制剂在啮齿动物大脑微注射

Here we describe a method for the construction of a single-use &#8220;injectrode&#8221; using commercially accessible and affordable parts. A probing ...

Personalized Needles for Microinjections in the Rodent Brain

啮齿动物脑 Microinjections 的个性化针

Microinjections have been used for a long time for the delivery of drugs or toxins within specific brain areas and, more recently, they have been used to ...

Evaluation of Synapse Density in Hippocampal Rodent Brain Slices

海马鼠脑切片的突触密度评价

In the brain, synapses are specialized junctions between neurons, determining the strength and spread of neuronal signaling. The number of synapses is ...

Simultaneous Cryosectioning of Multiple Rodent Brains

多鼠脑的同时 Cryosectioning

Histology and immunohistochemistry are routine methods of analysis to visualize microscopic anatomy and localize proteins within biological tissue. In ...