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DOI :
10.3791/1902-v
December 24th, 2010
Chapters
0:05
Title
1:03
Introduction
1:38
Injection of Caged Fluorescein
3:15
Mounting of Injected Embryos
4:17
Photoactivation
5:59
Detection of Uncaged Fluorescein
8:02
Tracing the Lineage of Neural Progenitors Using Photoactivation
8:45
Conclusion
多光子顕微鏡は深く、光浸透と低光毒性を持つ低エネルギーの光子の制御が可能になります。我々は、ゼブラフィッシュ胚における生細胞標識のためのこの技術の使用について説明します。このプロトコルは、容易に様々な光反応性分子の光誘導に適合させることができる。
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