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DOI :
10.3791/1902-v
December 24th, 2010
Chapters
0:05
Title
1:03
Introduction
1:38
Injection of Caged Fluorescein
3:15
Mounting of Injected Embryos
4:17
Photoactivation
5:59
Detection of Uncaged Fluorescein
8:02
Tracing the Lineage of Neural Progenitors Using Photoactivation
8:45
Conclusion
Multiphoton 현미경은 광학 깊이 침투 및 감소 phototoxicity 낮은 에너지 광자를 제어할 수 있습니다. 우리는 zebrafish 배아에 살고 세포 라벨이 기술의 사용을 설명합니다. 이 프로토콜은 쉽게 다양한 가벼운 반응 분자의 사진 유도를 위해 적용할 수 있습니다.
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