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DOI :
10.3791/1904-v
•
10:34 min
April 14th, 2010
Chapters
0:49
Intro
0:00
Title
1:16
Preparing and Counting the Namalwa Cells
4:17
Electroporating the Cells in 96-well Plates
5:55
Extract and Check RNA
7:54
Real-time PCR
8:58
Analysis of Gene Expression
10:03
Conclusion
此过程介绍了一个快速简便的工作流程,引入困难的siRNA转染细胞系,并按照实时PCR基因表达。使用自动细胞计数器,多井的电板,全自动电泳站提供快速和可靠的结果,而不需要昂贵的机械手。
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