Name: thin sectioning of slice preparations for immunohistochemistry
Uploaded: 2007-04-28T00:00:00
Description: Watch this Scientific Journal Video about Thin Sectioning of Slice Preparations for Immunohistochemistry at JoVE.com

<ol><li>准备从磁带华侨城平台的模具。 </li><li>与华侨城填充模具。冻结范围内的低温恒温器或通过使用粉碎的干冰。 </li><li>删除冻结华侨城平台周围的磁带。 </li><li>将冻结夹头和低温恒温器的安装阶段，并锁定在夹头的标记。 </li><li>组通过OCT平台，直到表面是平坦的。 </li><li>低温恒温器冻结阶段，取出重铺华侨城平台和地点。 </li><li>华侨城广场组织样本（以前用30％甘油或蔗糖的PBS冻存）。 </li><li>准备冻结列?...

<ol>
	<li>Scoutern, C. W., O'Connor, R., Cunningham, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Perfusion+fixation+of+research+animals.">Perfusion fixation of research animals.</a> <em style='font-style: italic'>Microsc. Today</em>. <b>14</b>, 26-33 (2006).</li><li>Cunningham, M. G., Connor, C. M., Zhang, K., Benes, F. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Diminished+serotonergic+innervation+of+adult+medial+prefrontal+cortex+after+6-OHDA+lesions+in+the+newborn+rat.">Diminished serotonergic innervation of adult medial prefrontal cortex after 6-OHDA lesions in the newborn rat.</a> <em style='font-style: italic'>Brain Res. Dev. Brain Res</em>. <b>157</b>, 124-131 (2005).</li></ol>

<p>这里介绍的协议为研究人员提供一个简洁，易于遵循大纲如何获得小，难以管理，组织块，如活检和大脑切片进行进一步研究，如薄的低温恒温器部分各种染色方法，原位杂交，免疫组织化学。 </p>

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thin sectioning of slice preparations for immunohistochemistry

<p class="jove_content">在神经科学，以及其他学科的许多调查，参与研究的小，但新鲜宏观件或部分组织已被保存下来，删除，或切除，但保持可行的，在脑组织切片准备。随后的微观研究，这种材料可以是具有挑战性，组织样本可能难以处理。证明这里是一个薄低温恒温器的部分，厚度为0.2-5.0毫米的范围可以从组织获得的方法。我们经常削减400微米厚Vibratome的脑片串联成5-10微米日冕低温恒温器的部分。切片通常首先用于电生理实验，然后需要的地区，从录音，观察细胞结构的微观分析。我们已经构建了一个简单的装置，可控制和可重复性的准备和组织切片定位。该装置由一个圆柱体5厘米的长度，用直径1.2厘米，作为冻结切片阶段。一环，紧贴幻灯片在气缸周围墙壁，让沉浸在冻结复合组织（例如，华侨城）切片。然后迅速冷冻粉碎的干冰和由此产生的晶圆可低温恒温切片的位置容易。薄片可以解冻安装在涂幻灯片，以便进一步的研究来进行，如各种染色方法，原位杂交，免疫组织化学，这里展示。</p>

Watch this Scientific Journal Video about Thin Sectioning of Slice Preparations for Immunohistochemistry at JoVE.com

Thin Sectioning of Slice Preparations for Immunohistochemistry

<p class="jove_content">本方法可重复性的低温恒温器的小，难以管理，组织块，如活组织切片检查和脑切片，切片。我们利用一个简单的铝冻结的阶段，以方便处理组织和一个标准的低温​​恒温器，定期制作5-10微米，400微米厚的脑片连续切片。</p>

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The Drosophila neuromuscular junction (NMJ) is an established model system used for the study of synaptic development and plasticity. The widespread use ...

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Drosophila has long been used as model system to study development, mainly due to the ease with which it is genetically tractable. Over the years, a ...

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The  tissue microarray (TMA) technology provides the means for high-throughput  analysis of multiple tissues and cells. The technique is used within the ...

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Working with delicate tissue can be a complicating factor when performing immunohistochemical assessment. Here, we present a method that utilizes a ...

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Multiple approaches have been used to record and evaluate gastrointestinal motility including: recording changes in muscle tension, intraluminal pressure, ...

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Methylation of cytosine bases (5-methylcytosine, 5mC) occurring in vertebrate genomes is usually associated with transcriptional silencing. ...