Name: direct tracheal instillation of solutes into mouse lung
Uploaded: 2010-08-29T17:00:00
Description: Watch this Scientific Journal Video about Direct Tracheal Instillation of Solutes into Mouse Lung at JoVE.com

Dit werk werd ondersteund door NIH K99 HL09222601 en de S &amp; R Foundation Ryaji Uneo Award uitgereikt aan MNH.

1. Anesthesie <ol><li> Verlamming muizen met isofluraan kunnen vergemakkelijken dier behandeling en terughoudendheid vóór het toedienen van anesthesie intraperitaneally. Met behulp van een geïnhaleerd verdoving kan wel of niet geschikt zijn voor een bepaalde studie: bepaal eerst de invloed van het gebruik van isofluraan, of enige andere verdoving, over het voorgestelde longen te bestuderen. </li><li> Verdoven dieren met isofluraan. De video toont het gebruik van een vaporizer isofluraan kamer vastgesteld op 2% isof...

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	<li>Driscoll, K. E., Costa, D. L., Hatch, G., Henderson, R., Oberdorster, G., Salem, H., Schlesinger, R. B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Intratracheal+instillation+as+an+exposure+technique+for+the+evaluation+of+respiratory+tract+toxicity:+uses+and+limitations.">Intratracheal instillation as an exposure technique for the evaluation of respiratory tract toxicity: uses and limitations.</a> Toxicol Sci. 55, 24-35 (2000).</li><li>Helms, M. N., Jain, L., Self, J. L., Eaton, D. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Redox+regulation+of+epithelial+sodium+channels+examined+in+alveolar+type+1+and+2+cells+patch-clamped+in+lung+slice+tissue.">Redox regulation of epithelial sodium channels examined in alveolar type 1 and 2 cells patch-clamped in lung slice tissue.</a> J Biol Chem. 283, 22875-22883 (2008).</li><li>Helms, M. N., Self, J., Bao, H. F., Job, L. C., Jain, L., Eaton, D. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Dopamine+activates+amiloride-sensitive+sodium+channels+in+alveolar+type+I+cells+in+lung+slice+preparations.">Dopamine activates amiloride-sensitive sodium channels in alveolar type I cells in lung slice preparations.</a> Am J Physiol Lung Cell Mol Physiol. 291, L610-L618 (2006).</li><li>Iyer, S. S., Ramirez, A. M., Ritzenthaler, J. D., Torres-Gonzalez, E., Roser-Page, S., Mora, A. L., Brigham, K. L., Jones, D. P., Roman, J., Rojas, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Oxidation+of+extracellular+cysteine/cystine+redox+state+in+bleomycin-induced+lung+fibrosis.">Oxidation of extracellular cysteine/cystine redox state in bleomycin-induced lung fibrosis.</a> Am J Physiol Lung Cell Mol Physiol. 296, L37-L45 (2009).</li><li>Li, T., Folkesson, H. G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=RNA+interference+for+alpha-ENaC+inhibits+rat+lung+fluid+absorption+in+vivo.">RNA interference for alpha-ENaC inhibits rat lung fluid absorption in vivo.</a> Am J Physiol Lung Cell Mol Physiol. 290, L649-L660 (2006).</li><li>Sager, T., Porter, D., Robinson, V., Lindsley, W., Schwegler-Berry, D., Castranova, V. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=An+improved+method+to+disperse+nanoparticles+for+in+vitro+and+in+vivo+investigation+of+toxicity.">An improved method to disperse nanoparticles for in vitro and in vivo investigation of toxicity.</a> Nanotoxicol. 1, 118-129 (2007).</li><li>Xu, J., Gonzalez, E. T., Iyer, S. S., Mac, V., Mora, A. L., Sutliff, R. L., Reed, A., Brigham, K. L., Kelly, P., Rojas, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Use+of+senescence-accelerated+mouse+model+in+bleomycin-induced+lung+injury+suggests+that+bone+marrow-derived+cells+can+alter+the+outcome+of+lung+injury+in+aged+mice.">Use of senescence-accelerated mouse model in bleomycin-induced lung injury suggests that bone marrow-derived cells can alter the outcome of lung injury in aged mice.</a> J Gerontol A Biol Sci Med Sci. 64, 731-739 (2009).</li><li>Yeh, T. F., Lin, H. C., Chang, C. H., Wu, T. S., Su, B. H., Li, T. C., Pyati, S., Tsai, C. H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Early+intratracheal+instillation+of+budesonide+using+surfactant+as+a+vehicle+to+prevent+chronic+lung+disease+in+preterm+infants:+a+pilot+study.">Early intratracheal instillation of budesonide using surfactant as a vehicle to prevent chronic lung disease in preterm infants: a pilot study.</a> Pediatrics. 121, e1310-e1318 (2008).</li></ol>

Intratracheale instillaties zijn gebruikt in een aantal verschillende studies naar de toxiciteit van stoffen te testen (6) te evalueren, alveolaire longbeschadiging (4, 7) te induceren, oppervlakte-actieve stoffen (8), evenals alter genexpressie direct te vervangen door de levering van kleine oligonucleotiden in de long (5). We zijn momenteel het verkennen van het gebruik van de bevolking is 1) TL-redox-gevoelig materiaal in de longen om oxidatieve stress te meten in vivo onder normale en pathofysiologische omstandighed...

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		<th>Catalogue Number</th>
		<th>Comment</th>
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<tr>
<td>Isoflurane	</td>
<td>&nbsp;</td>
<td>Webster Veterinary Sterling , MA</td>
<td>14043-220-05</td>
<td>&nbsp;</td>
<tr>
<td>Xylazine</td>
<td>&nbsp;</td>
<td>Lloyd Laboratories Shenandoah, IA</td>
<td>&nbsp;</td>
<td>New Animal Drug Application #139-236</td>
</tr>
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<td>Ketamine</td>
<td>&nbsp;</td>
<td>Bioniche Pharma Lake Forest, IL</td>
<td>67457-001-10</td>
<td>&nbsp;</td>
<tr>
<td>Nair</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>Available at drug stores</td>
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<td>1mL SubQ Syringe</td>
<td>&nbsp;</td>
<td>BD Syringe Franklin Lakes, NJ</td>
<td>309597</td>
<td>26 5/8 G</td>
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<td>4-0 Nylon Suture</td>
<td>&nbsp;</td>
<td>Ethicon San Lorenzo, Puerto Rico</td>
<td>1894G.S30</td>
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direct tracheal instillation of solutes into mouse lung

Intratracheale instillaties leveren opgeloste stoffen direct in de longen. Deze procedure richt zich op de levering van de instillate in het distale gebieden van de long, en is daarom vaak opgenomen in studies gericht op het bestuderen van longblaasjes. Wij bieden een uitgebreide survival protocol voor het uitvoeren van intratracheale instillaties bij muizen. Met behulp van deze aanpak, kan men de levering van test-opgeloste stoffen of vaste stoffen (zoals long-therapeutica, oppervlakteactieve stoffen, virussen, en kleine oligonucleotiden) te richten op de distale long. Tracheale instillaties kan de voorkeur methodiek worden, na inhalatie-protocollen die in de eerste plaats kunnen richten op de bovenste luchtwegen en eventueel de onderzoeker bloot aan potentieel gevaarlijke stoffen. Bovendien, het gebruik van de tracheale instillatie protocol, kunnen dieren volledig te herstellen van de niet-invasieve procedure. Dit maakt voor het maken van de volgende fysiologische metingen op proefdieren, of opnieuw installeren met behulp van hetzelfde dier. De hoeveelheid instillate geïntroduceerd in de longen moet zorgvuldig worden bepaald en osmotisch evenwicht van dieren herstel te verzekeren. Meestal kan 30 tot 75 pi instillate volume worden opgenomen in de muis long.

Watch this Scientific Journal Video about Direct Tracheal Instillation of Solutes into Mouse Lung at JoVE.com

Direct Tracheal Instillation of Solutes into Mouse Lung

Intratracheale instillaties leveren opgeloste stoffen direct in de longen. Deze procedure richt zich op de levering van de instillate in het distale gebieden van de long, en is daarom vaak opgenomen in studies gericht op het bestuderen van longblaasjes. Wij bieden een uitgebreide survival protocol voor het uitvoeren van intratracheale instillaties bij muizen.

Directe Tracheale Instillatie van opgeloste stoffen in de muis Lung

Intratracheal instillations deliver solutes directly into the lungs.  This procedure targets the delivery of the instillate into the distal regions of the ...

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