Name: direct tracheal instillation of solutes into mouse lung
Uploaded: 2010-08-29T17:00:00
Description: Watch this Scientific Journal Video about Direct Tracheal Instillation of Solutes into Mouse Lung at JoVE.com

この作品は、NIH K99 HL09222601とMNHに贈られるS＆R財団Ryaji Uneo賞によってサポートされていました。

1。麻酔<ol><li>イソフルランでマウスを麻酔するintraperitaneally麻酔を投与する前に、動物取扱と自制を促進することができる。吸入麻酔薬を使用すると、または特定の研究に適していない場合があります。提案された肺の研究の最初のイソフルランを使用しての衝撃、またはその他の麻酔薬を、決定する。 </li><li>イソフルランで動物を麻酔。ビデオでは、酸素と混合イソフルラン2％で設定さ...

<ol>
	<li>Driscoll, K. E., Costa, D. L., Hatch, G., Henderson, R., Oberdorster, G., Salem, H., Schlesinger, R. B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Intratracheal+instillation+as+an+exposure+technique+for+the+evaluation+of+respiratory+tract+toxicity:+uses+and+limitations.">Intratracheal instillation as an exposure technique for the evaluation of respiratory tract toxicity: uses and limitations.</a> Toxicol Sci. 55, 24-35 (2000).</li><li>Helms, M. N., Jain, L., Self, J. L., Eaton, D. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Redox+regulation+of+epithelial+sodium+channels+examined+in+alveolar+type+1+and+2+cells+patch-clamped+in+lung+slice+tissue.">Redox regulation of epithelial sodium channels examined in alveolar type 1 and 2 cells patch-clamped in lung slice tissue.</a> J Biol Chem. 283, 22875-22883 (2008).</li><li>Helms, M. N., Self, J., Bao, H. F., Job, L. C., Jain, L., Eaton, D. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Dopamine+activates+amiloride-sensitive+sodium+channels+in+alveolar+type+I+cells+in+lung+slice+preparations.">Dopamine activates amiloride-sensitive sodium channels in alveolar type I cells in lung slice preparations.</a> Am J Physiol Lung Cell Mol Physiol. 291, L610-L618 (2006).</li><li>Iyer, S. S., Ramirez, A. M., Ritzenthaler, J. D., Torres-Gonzalez, E., Roser-Page, S., Mora, A. L., Brigham, K. L., Jones, D. P., Roman, J., Rojas, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Oxidation+of+extracellular+cysteine/cystine+redox+state+in+bleomycin-induced+lung+fibrosis.">Oxidation of extracellular cysteine/cystine redox state in bleomycin-induced lung fibrosis.</a> Am J Physiol Lung Cell Mol Physiol. 296, L37-L45 (2009).</li><li>Li, T., Folkesson, H. G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=RNA+interference+for+alpha-ENaC+inhibits+rat+lung+fluid+absorption+in+vivo.">RNA interference for alpha-ENaC inhibits rat lung fluid absorption in vivo.</a> Am J Physiol Lung Cell Mol Physiol. 290, L649-L660 (2006).</li><li>Sager, T., Porter, D., Robinson, V., Lindsley, W., Schwegler-Berry, D., Castranova, V. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=An+improved+method+to+disperse+nanoparticles+for+in+vitro+and+in+vivo+investigation+of+toxicity.">An improved method to disperse nanoparticles for in vitro and in vivo investigation of toxicity.</a> Nanotoxicol. 1, 118-129 (2007).</li><li>Xu, J., Gonzalez, E. T., Iyer, S. S., Mac, V., Mora, A. L., Sutliff, R. L., Reed, A., Brigham, K. L., Kelly, P., Rojas, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Use+of+senescence-accelerated+mouse+model+in+bleomycin-induced+lung+injury+suggests+that+bone+marrow-derived+cells+can+alter+the+outcome+of+lung+injury+in+aged+mice.">Use of senescence-accelerated mouse model in bleomycin-induced lung injury suggests that bone marrow-derived cells can alter the outcome of lung injury in aged mice.</a> J Gerontol A Biol Sci Med Sci. 64, 731-739 (2009).</li><li>Yeh, T. F., Lin, H. C., Chang, C. H., Wu, T. S., Su, B. H., Li, T. C., Pyati, S., Tsai, C. H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Early+intratracheal+instillation+of+budesonide+using+surfactant+as+a+vehicle+to+prevent+chronic+lung+disease+in+preterm+infants:+a+pilot+study.">Early intratracheal instillation of budesonide using surfactant as a vehicle to prevent chronic lung disease in preterm infants: a pilot study.</a> Pediatrics. 121, e1310-e1318 (2008).</li></ol>

気管内点滴が試験化合物（6）の毒性を評価するためにいくつかの様々な研究で使用されている、肺胞肺傷害（4、7）を誘導、界面活性剤（8）を置き換えるだけでなく、直接に小さなオリゴヌクレオチドの配信を介して遺伝子発現を変化させる肺（5）。我々は現在、肺への蛍光レドックス感受性材料は薬理学的化合物、DNAベクター、ウイルス、およびで蛍光指示薬を浸透させる）だけでなく?...

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<td>Isoflurane	</td>
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<td>Xylazine</td>
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<td>Nair</td>
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direct tracheal instillation of solutes into mouse lung

気管内点滴は直接肺に溶質を提供します。この手順では、肺の遠位領域にinstillateの配信をターゲットとし、従って多くの場合、肺胞の勉強を目的とした研究に組み込まれています。我々は、マウスで気管内点滴を行うための詳細な生存のプロトコルを提供しています。このアプローチを使用して、一つは遠位肺に（例えば、肺の治療薬、界面活性剤、ウイルス、および小規模なオリゴヌクレオチドとして）テストの溶質または固体の配信をターゲットにすることができます。気管点滴は、主に上気道を対象に、おそらく潜在的に有害物質への捜査官を公開することができる吸入プロトコルを介して、好みの方法論があります。さらに、気管注入プロトコルを使用して、動物は完全に非侵襲的な手順から回復することができます。これは、同じ動物を用いた試験の動物、または再インストール時にも、その後の生理的な測定をすることが可能になる。肺に導入instillateの量は、慎重に決定され、動物の回復を確実にするために浸透圧バランスする必要があります。通常は、30から75μLinstillateのボリュームは、マウスの肺に導入することができる。

Watch this Scientific Journal Video about Direct Tracheal Instillation of Solutes into Mouse Lung at JoVE.com

Direct Tracheal Instillation of Solutes into Mouse Lung

気管内点滴は直接肺に溶質を提供します。この手順では、肺の遠位領域にinstillateの配信をターゲットとし、従って多くの場合、肺胞の勉強を目的とした研究に組み込まれています。我々は、マウスで気管内点滴を行うための詳細な生存のプロトコルを提供しています。

マウス肺への溶質の直接気管点眼

Intratracheal instillations deliver solutes directly into the lungs.  This procedure targets the delivery of the instillate into the distal regions of the ...

Research

JoVE Journal

Biology

Nuclear Transfer into Mouse Oocytes

マウス卵母細胞への核移転

Nuclear transfer into an unfertilized oocyte can restore developmental potential to a differentiated cell. This demonstrates that the processes underlying ...

Standardized Preparation of Single-Cell Suspensions from Mouse Lung Tissue using the gentleMACS Dissociator

gentleMACS Dissociatorを使用してマウスの肺組織からの単細胞懸濁液の標準化の準備

The preparation of single-cell suspensions from tissues is an important prerequisite for many experiments in cellular research. The process of ...

Mouse Embryonic Lung Culture, A System to Evaluate the Molecular Mechanisms of Branching

マウス胎児肺の文化、分岐の分子機構を評価するシステム

Lung primordial specification as well as branching morphogenesis, and the formation of various pulmonary cell lineages requires a specific interaction of ...

In vivo Imaging and Therapeutic Treatments in an Orthotopic Mouse Model of Ovarian Cancer

In vivo Imaging and Therapeutic Treatments in an Orthotopic Mouse Model of Ovarian Cancer

生体イメージングおよび卵巣癌の同所性マウスモデルにおける治療的処置の

Human cancer and response to therapy is better represented in orthotopic animal models.  This paper describes the development of an orthotopic mouse model ...

Direct Delivery of MIF Morpholinos Into the Zebrafish Otocyst by Injection and Electroporation Affects Inner Ear Development

注射とエレクトロポレーションによりゼブラフィッシュ耳胞イントゥMIFモルフォの直接配信は、内耳の開発に影響を与える

In recent years, electroporation has become a popular technique for in vivo transfection of DNA, RNA, and morpholinos into various tissues, including the ...

Characterization of the Isolated, Ventilated, and Instrumented Mouse Lung Perfused with Pulsatile Flow

絶縁型、ベンチレーテッド、および計装のマウス肺の特性評価は、拍動流を灌流

The isolated, ventilated and instrumented mouse lung preparation allows steady and pulsatile pulmonary vascular pressure-flow relationships to be measured ...

Isolation &#38; Characterization of Hoechstlow CD45negative Mouse Lung Mesenchymal Stem Cells

Isolation & Characterization of Hoechstlow CD45negative Mouse Lung Mesenchymal Stem Cells

ヘキストの分離および特性評価低 CD45負マウス肺間葉系幹細胞

Tissue resident mesenchymal stem cells (MSC) are important regulators of tissue repair or regeneration, fibrosis, inflammation, angiogenesis and tumor ...

Efficient Differentiation of Mouse Embryonic Stem Cells into Motor Neurons

運動ニューロンにマウス胚性幹細胞の効率的な分化

Direct differentiation of embryonic stem (ES) cells into functional  motor neurons represents a promising resource to study disease mechanisms, to  screen ...

Generation of Mice Derived from Induced Pluripotent Stem Cells

人工多能性幹細胞に由来するマウスの作製

The production of induced pluripotent stem cells (iPSCs) from somatic cells provides a means to create valuable tools for basic research and may also ...

Protein Transfection of Mouse Lung

マウス肺のタンパク質トランスフェクション

Increasing protein expression enables researchers to  better understand the functional role of that protein in regulating key  biological processes1. In ...