Name: chronic salmonella infected mouse model
Uploaded: 2010-05-31T17:00:00
Description: Watch this Scientific Journal Video about Chronic Salmonella Infected Mouse Model at JoVE.com

Dit werk werd ondersteund door de NIDDK KO1 DK075386 te verlenen en de American Cancer Society RSG-09-075-01-MBC tot Jun zon

Dit protocol omvat drie delen: bacteriecultuur, muis maagsonde, en Salmonella detectie. 1. Salmonella groei conditie. <ol><li> Bereid de Salmonella Luria-Bertani bouillon (LB) Plaat uit, incubeer bij 37 ° C gedurende de nacht. </li><li> Kies een kloon van de LB plaat en in 7 ml LB in een 12 ml tube, en schud bij 37 ° C gedurende ongeveer 5 uur. </li><li> Inoculeren 50 ml LB met 0,05 ml van een stationaire fase cultuur en incubeer bij 37 ° C zonder schudden ged...

<ol>
	<li>Duan, Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=beta-Catenin+activity+negatively+regulates+bacteria-induced+inflammation.">beta-Catenin activity negatively regulates bacteria-induced inflammation.</a> Lab Invest. 87 (6), 613-624 (2007).</li><li>Barthel, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Pretreatment+of+mice+with+streptomycin+provides+a+Salmonella+enterica+serovar+Typhimurium+colitis+model+that+allows+analysis+of+both+pathogen+and+host.">Pretreatment of mice with streptomycin provides a Salmonella enterica serovar Typhimurium colitis model that allows analysis of both pathogen and host.</a> Infection and immunity. 71 (5), 2839-2858 (2003).</li><li>Miller, S. I., Mekalanos, J. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Constitutive+expression+of+the+phoP+regulon+attenuates+Salmonella+virulence+and+survival+within+macrophages.">Constitutive expression of the phoP regulon attenuates Salmonella virulence and survival within macrophages.</a> Journal of bacteriology. 172 (5), 2485-2490 (1990).</li><li>Valdez, Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Nramp1+expression+by+dendritic+cells+modulates+inflammatory+responses+during+Salmonella+Typhimurium+infection.">Nramp1 expression by dendritic cells modulates inflammatory responses during Salmonella Typhimurium infection.</a> Cell Microbiol. 10 (8), 1646-1661 (2008).</li><li>Woo, H., Okamoto, S., Guiney, D., Gunn, J. S., Fierer, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+model+of+Salmonella+colitis+with+features+of+diarrhea+in+SLC11A1+wild-type+mice.">A model of Salmonella colitis with features of diarrhea in SLC11A1 wild-type mice.</a> PLoS ONE. 3 (2), e1603-e1603 (2008).</li><li>Dangl, J. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Molecular+call-and-response:+how+Salmonella+learns+the+gospel+from+its+host.">Molecular call-and-response: how Salmonella learns the gospel from its host.</a> Trends Microbiol. 11 (6), 245-246 (2003).</li><li>Zaharik, M. L., Vallance, B. A., Puente, J. L., Gros, P., Finlay, B. 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L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Humane+endpoints+for+infectious+disease+animal+models.">Humane endpoints for infectious disease animal models.</a> ILAR J. 41 (2), 99-104 (2000).</li><li>Ye, Z., Petrof, E. O., Boone, D., Claud, E. C., Sun, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Salmonella+effector+AvrA+regulation+of+colonic+epithelial+cell+inflammation+by+deubiquitination.">Salmonella effector AvrA regulation of colonic epithelial cell inflammation by deubiquitination.</a> Am J Pathol. 171 (3), 882-892 (2007).</li><li>Grassl, G. A., Valdez, Y., Bergstrom, K. S., Vallance, B. A., Finlay, B. B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Chronic+enteric+salmonella+infection+in+mice+leads+to+severe+and+persiste+nt+intestinal+fibrosis.">Chronic enteric salmonella infection in mice leads to severe and persiste nt intestinal fibrosis.</a> Gastroenterology. 134 (3), 768-780 (2008).</li><li>Sukupolvi, S., Edelstein, A., Rhen, M., Normark, S. J., Pfeifer, J. 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Voor het gebruik van dit systeem, is het noodzakelijk dat de onderzoeker om te leren hoe de dieren maagsonde. We detail een methodiek voor te bereiden bacteriecultuur en maagsonde de muizen. We hebben ook laten zien hoe je de Salmonella persistentie in de gastro-intestinale (GI) stelsel te controleren. De kritische stappen in dit protocol, waaronder: <ol><li> Streptomycine-voorbehandeling: streptomycine-voorbehandeling kan ontdoen van een aantal commensale darmflora en maken de muizen gevoelig zijn voor de ...

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		<th>Material Name</th>
		<th>Type</th>
		<th>Company</th>
		<th>Catalogue Number</th>
		<th>Comment</th>
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<tr>
<td>HBSS</td>
<td>&nbsp;</td>
<td>Sigma</td>
<td>ABCD1234</td>
<td>&nbsp;</td>
<tr>
<td>Luria-Bertani broth</td>
<td>&nbsp;</td>
<td>BD</td>
<td>244610</td>
<td>&nbsp;</td>
<tr>
<td>Feeding needle</td>
<td>&nbsp;</td>
<td>Popper &amp; Sons, Inc</td>
<td>7920</td>
<td>&nbsp;</td>
<tr>
<td>Streptomycin </td>
<td>&nbsp;</td>
<td>MP biomedicals, LLC</td>
<td>100556</td>
<td>&nbsp;</td>
<tr>
<td>BBL CHROMagar Salmonella</td>
<td>&nbsp;</td>
<td>BD</td>
<td>214983</td>
<td>&nbsp;</td>
<tr>
<td>Disposable cell spreaders</td>
<td>&nbsp;</td>
<td>Biologix research company</td>
<td>65-1010</td>
<td>&nbsp;</td>		</tbody>
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chronic salmonella infected mouse model

De bacteriële infectie muis model is een krachtig modelsysteem voor de bestudering van gebieden, zoals infecties, ontstekingen, immunologie, signaaltransductie, en tumorigenese. Veel onderzoekers hebben gebruik gemaakt van de colitis veroorzaakt door<em&gt; Salmonella</em&gt; Typhimurium voor de studies op de vroege fase van de ontsteking en infectie. Echter, slechts weinig rapporten zijn op de chronische infectie<em&gt; In vivo</em&gt;. Muizen met<em&gt; Salmonella</em&gt; Aanhoudende bestaan ​​in het maag-darmkanaal kunnen we op de lange termijn gastheer-bacteriële interactie, signaaltransductie, en tumorigenese te verkennen. We hebben een chronische bacteriële infectie muismodel met<em&gt; Salmonella</em&gt; Typhimurium kolonisatie in de muis darm meer dan 6 maanden. Voor het gebruik van dit systeem, is het noodzakelijk dat de onderzoeker om te leren hoe de bacteriële cultuur voor te bereiden en maagsonde de dieren. We detail een methodiek voor te bereiden bacteriecultuur en maagsonde muizen. We hebben ook laten zien hoe je de sporen<em&gt; Salmonella</em&gt; Persistentie in het maagdarmkanaal. In het algemeen zal dit protocol hulp onderzoekers met behulp van de bacteriële infectie muismodel van fundamenteel belang biologische en microbiologische vragen te beantwoorden.

Watch this Scientific Journal Video about Chronic Salmonella Infected Mouse Model at JoVE.com

Chronic Salmonella Infected Mouse Model

Stel een chronische bacteriële infectie muismodel met persisterende<em&gt; Salmonella</em&gt; Typhimurium kolonisatie in de darm voor 27 weken.

Chronisch Salmonella Infected Muis Model

The bacterial infected mouse model is a powerful model system for studying areas such as infection, inflammation, immunology, signal transduction, and ...

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Biology

Chronic Salmonella Infected Mouse Model

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