Name: chronic salmonella infected mouse model
Uploaded: 2010-05-31T17:00:00
Description: Watch this Scientific Journal Video about Chronic Salmonella Infected Mouse Model at JoVE.com

Diese Arbeit wurde von der NIDDK KO1 DK075386 gewähren und die American Cancer Society RSG-09-075 bis 01-MBC bis Jun Sun unterstützt.

Dieses Protokoll umfasst drei Teile: Bakterienkultur, Maus Magensonde und Nachweis von Salmonellen. 1. Salmonella Wachstum Zustand. <ol><li> Bereiten Sie die Salmonella Luria-Bertani Broth (LB)-Platte, bei 37 ° C über Nacht inkubieren. </li><li> Wählen Sie einen Klon aus der LB-Platte und in 7 ml LB in einer 12 ml-Tube, und schütteln bei 37 ° C für ca. 5 Stunden. </li><li> Inoculate 50 ml LB mit 0,05 ml einer stationären Phase der Kultur und bei 37 ° C oh...

<ol>
	<li>Duan, Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=beta-Catenin+activity+negatively+regulates+bacteria-induced+inflammation.">beta-Catenin activity negatively regulates bacteria-induced inflammation.</a> Lab Invest. 87 (6), 613-624 (2007).</li><li>Barthel, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Pretreatment+of+mice+with+streptomycin+provides+a+Salmonella+enterica+serovar+Typhimurium+colitis+model+that+allows+analysis+of+both+pathogen+and+host.">Pretreatment of mice with streptomycin provides a Salmonella enterica serovar Typhimurium colitis model that allows analysis of both pathogen and host.</a> Infection and immunity. 71 (5), 2839-2858 (2003).</li><li>Miller, S. I., Mekalanos, J. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Constitutive+expression+of+the+phoP+regulon+attenuates+Salmonella+virulence+and+survival+within+macrophages.">Constitutive expression of the phoP regulon attenuates Salmonella virulence and survival within macrophages.</a> Journal of bacteriology. 172 (5), 2485-2490 (1990).</li><li>Valdez, Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Nramp1+expression+by+dendritic+cells+modulates+inflammatory+responses+during+Salmonella+Typhimurium+infection.">Nramp1 expression by dendritic cells modulates inflammatory responses during Salmonella Typhimurium infection.</a> Cell Microbiol. 10 (8), 1646-1661 (2008).</li><li>Woo, H., Okamoto, S., Guiney, D., Gunn, J. S., Fierer, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+model+of+Salmonella+colitis+with+features+of+diarrhea+in+SLC11A1+wild-type+mice.">A model of Salmonella colitis with features of diarrhea in SLC11A1 wild-type mice.</a> PLoS ONE. 3 (2), e1603-e1603 (2008).</li><li>Dangl, J. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Molecular+call-and-response:+how+Salmonella+learns+the+gospel+from+its+host.">Molecular call-and-response: how Salmonella learns the gospel from its host.</a> Trends Microbiol. 11 (6), 245-246 (2003).</li><li>Zaharik, M. L., Vallance, B. A., Puente, J. L., Gros, P., Finlay, B. B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Host-pathogen+interactions:+Host+resistance+factor+Nramp1+up-regulates+the+expression+of+Salmonella+pathogenicity+island-2+virulence+genes.">Host-pathogen interactions: Host resistance factor Nramp1 up-regulates the expression of Salmonella pathogenicity island-2 virulence genes.</a> Proceedings of the National Academy of Sciences of the United States of America. 99, 15705-15710 (2002).</li><li>Townsend, P., Morton, D. B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Laboratory+Animal+Care+Policies+and+Regulations:+United+Kingdom.">Laboratory Animal Care Policies and Regulations: United Kingdom.</a> ILAR J. 37 (2), 68-74 (1995).</li><li>Olfert, E. D., Godson, D. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Humane+endpoints+for+infectious+disease+animal+models.">Humane endpoints for infectious disease animal models.</a> ILAR J. 41 (2), 99-104 (2000).</li><li>Ye, Z., Petrof, E. O., Boone, D., Claud, E. C., Sun, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Salmonella+effector+AvrA+regulation+of+colonic+epithelial+cell+inflammation+by+deubiquitination.">Salmonella effector AvrA regulation of colonic epithelial cell inflammation by deubiquitination.</a> Am J Pathol. 171 (3), 882-892 (2007).</li><li>Grassl, G. A., Valdez, Y., Bergstrom, K. S., Vallance, B. A., Finlay, B. B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Chronic+enteric+salmonella+infection+in+mice+leads+to+severe+and+persiste+nt+intestinal+fibrosis.">Chronic enteric salmonella infection in mice leads to severe and persiste nt intestinal fibrosis.</a> Gastroenterology. 134 (3), 768-780 (2008).</li><li>Sukupolvi, S., Edelstein, A., Rhen, M., Normark, S. J., Pfeifer, J. D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Development+of+a+murine+model+of+chronic+Salmonella+infection.">Development of a murine model of chronic Salmonella infection.</a> Infection and immunity. 65 (2), 838-842 (1997).</li><li>. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> Biosafety in Microbiological and Biomedical Laboratories (BMBL). , (2007).</li><li>Wu, S., Lu, R., Zhang, Y., Sun, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Chronic+effects+of+a+Salmonella+type+III+secretion+effector+protein+AvrA+in+vivo.">Chronic effects of a Salmonella type III secretion effector protein AvrA in vivo.</a> PLoS ONE. , (2010).</li></ol>

Zur Nutzung dieses Systems ist es notwendig, für den Forscher lernen, wie man die Tiere eine Magensonde. Wir Detail eine Methodik für die Vorbereitung Bakterienkultur und Magensonde die Mäuse. Wir zeigen auch, wie die Salmonellen Persistenz im Magen-Darm (GI-Trakt) zu überwachen. Die kritischen Schritte in diesem Protokoll einschließlich: <ol><li> Streptomycin-Vorbehandlung: Streptomycin-Vorbehandlung loswerden könnte einige kommensalen Darmflora und machen die Mäuse anfällig für die Infektion mit ...

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		<th>Material Name</th>
		<th>Type</th>
		<th>Company</th>
		<th>Catalogue Number</th>
		<th>Comment</th>
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<td>HBSS</td>
<td>&nbsp;</td>
<td>Sigma</td>
<td>ABCD1234</td>
<td>&nbsp;</td>
<tr>
<td>Luria-Bertani broth</td>
<td>&nbsp;</td>
<td>BD</td>
<td>244610</td>
<td>&nbsp;</td>
<tr>
<td>Feeding needle</td>
<td>&nbsp;</td>
<td>Popper &amp; Sons, Inc</td>
<td>7920</td>
<td>&nbsp;</td>
<tr>
<td>Streptomycin </td>
<td>&nbsp;</td>
<td>MP biomedicals, LLC</td>
<td>100556</td>
<td>&nbsp;</td>
<tr>
<td>BBL CHROMagar Salmonella</td>
<td>&nbsp;</td>
<td>BD</td>
<td>214983</td>
<td>&nbsp;</td>
<tr>
<td>Disposable cell spreaders</td>
<td>&nbsp;</td>
<td>Biologix research company</td>
<td>65-1010</td>
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chronic salmonella infected mouse model

Die bakterielle Infektion Mausmodell ist ein leistungsfähiges Modell für die Erforschung von Bereichen wie Infektion, Entzündung, Immunologie, Signaltransduktion und Tumorgenese. Viele Forscher haben den Vorteil der Colitis induziert durch genommen<em&gt; Salmonellen</em&gt; Typhimurium für die Studien über die frühe Phase der Entzündung und Infektion. Allerdings sind nur wenige Berichte über die chronische Infektion<em&gt; In vivo</em&gt;. Mäuse, die mit<em&gt; Salmonellen</em&gt; Persistent Existenz im Magen-Darm-Trakt erlauben uns, die langfristige Wirt-Bakterien-Interaktion, der Signaltransduktion und Tumorgenese zu erkunden. Wir haben eine chronische bakterielle Infektion Mausmodell mit etablierten<em&gt; Salmonellen</em&gt; Typhimurium Kolonisierung in der Maus Darm über 6 Monate. Zur Nutzung dieses Systems ist es notwendig, für den Forscher lernen, wie man die Bakterienkultur vorzubereiten und eine Magensonde den Tieren. Wir Detail eine Methodik für die Vorbereitung Bakterienkultur und eine Magensonde Mäusen. Wir zeigen auch, wie die Erkennung<em&gt; Salmonellen</em&gt; Persistenz im Magen-Darm-Trakt. Insgesamt wird dieses Protokoll Hilfe Forscher mit der bakteriellen Infektion Mausmodell grundlegend wichtig, biologischen und mikrobiologischen Fragen zu beantworten.

Watch this Scientific Journal Video about Chronic Salmonella Infected Mouse Model at JoVE.com

Chronic Salmonella Infected Mouse Model

Stellen Sie eine chronische bakterielle Infektion Mausmodell mit persistierenden<em&gt; Salmonellen</em&gt; Typhimurium Kolonisation im Darm für 27 Wochen.

Chronisch Salmonellen Infected Maus-Modell

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Chronic Salmonella Infected Mouse Model

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