Name: chronic salmonella infected mouse model
Uploaded: 2010-05-31T17:00:00
Description: Watch this Scientific Journal Video about Chronic Salmonella Infected Mouse Model at JoVE.com

Questo lavoro è stato sostenuto dal KO1 NIDDK DK075386 concessione e l&#39;American Cancer Society RSG-09-075-01-MBC a giugno Dom

Questo protocollo comprende tre parti: rilevamento di coltura batterica, gavage mouse e Salmonella. 1. Salmonella condizione di crescita. <ol><li> Preparare la Salmonella brodo Luria-Bertani (LB) piastra, incubare a 37 ° C durante la notte. </li><li> Scegli un clone dalla piastra LB e mettere in 7 ml di LB in una provetta da 12 ml, e si agita a 37 ° C per circa 5 ore. </li><li> Inoculare 50 LB ml con 0,05 ml di una cultura fase stazionaria e incubare a 37 ° C ...

<ol>
	<li>Duan, Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=beta-Catenin+activity+negatively+regulates+bacteria-induced+inflammation.">beta-Catenin activity negatively regulates bacteria-induced inflammation.</a> Lab Invest. 87 (6), 613-624 (2007).</li><li>Barthel, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Pretreatment+of+mice+with+streptomycin+provides+a+Salmonella+enterica+serovar+Typhimurium+colitis+model+that+allows+analysis+of+both+pathogen+and+host.">Pretreatment of mice with streptomycin provides a Salmonella enterica serovar Typhimurium colitis model that allows analysis of both pathogen and host.</a> Infection and immunity. 71 (5), 2839-2858 (2003).</li><li>Miller, S. I., Mekalanos, J. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Constitutive+expression+of+the+phoP+regulon+attenuates+Salmonella+virulence+and+survival+within+macrophages.">Constitutive expression of the phoP regulon attenuates Salmonella virulence and survival within macrophages.</a> Journal of bacteriology. 172 (5), 2485-2490 (1990).</li><li>Valdez, Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Nramp1+expression+by+dendritic+cells+modulates+inflammatory+responses+during+Salmonella+Typhimurium+infection.">Nramp1 expression by dendritic cells modulates inflammatory responses during Salmonella Typhimurium infection.</a> Cell Microbiol. 10 (8), 1646-1661 (2008).</li><li>Woo, H., Okamoto, S., Guiney, D., Gunn, J. S., Fierer, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+model+of+Salmonella+colitis+with+features+of+diarrhea+in+SLC11A1+wild-type+mice.">A model of Salmonella colitis with features of diarrhea in SLC11A1 wild-type mice.</a> PLoS ONE. 3 (2), e1603-e1603 (2008).</li><li>Dangl, J. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Molecular+call-and-response:+how+Salmonella+learns+the+gospel+from+its+host.">Molecular call-and-response: how Salmonella learns the gospel from its host.</a> Trends Microbiol. 11 (6), 245-246 (2003).</li><li>Zaharik, M. L., Vallance, B. A., Puente, J. L., Gros, P., Finlay, B. B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Host-pathogen+interactions:+Host+resistance+factor+Nramp1+up-regulates+the+expression+of+Salmonella+pathogenicity+island-2+virulence+genes.">Host-pathogen interactions: Host resistance factor Nramp1 up-regulates the expression of Salmonella pathogenicity island-2 virulence genes.</a> Proceedings of the National Academy of Sciences of the United States of America. 99, 15705-15710 (2002).</li><li>Townsend, P., Morton, D. B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Laboratory+Animal+Care+Policies+and+Regulations:+United+Kingdom.">Laboratory Animal Care Policies and Regulations: United Kingdom.</a> ILAR J. 37 (2), 68-74 (1995).</li><li>Olfert, E. D., Godson, D. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Humane+endpoints+for+infectious+disease+animal+models.">Humane endpoints for infectious disease animal models.</a> ILAR J. 41 (2), 99-104 (2000).</li><li>Ye, Z., Petrof, E. O., Boone, D., Claud, E. C., Sun, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Salmonella+effector+AvrA+regulation+of+colonic+epithelial+cell+inflammation+by+deubiquitination.">Salmonella effector AvrA regulation of colonic epithelial cell inflammation by deubiquitination.</a> Am J Pathol. 171 (3), 882-892 (2007).</li><li>Grassl, G. A., Valdez, Y., Bergstrom, K. S., Vallance, B. A., Finlay, B. B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Chronic+enteric+salmonella+infection+in+mice+leads+to+severe+and+persiste+nt+intestinal+fibrosis.">Chronic enteric salmonella infection in mice leads to severe and persiste nt intestinal fibrosis.</a> Gastroenterology. 134 (3), 768-780 (2008).</li><li>Sukupolvi, S., Edelstein, A., Rhen, M., Normark, S. J., Pfeifer, J. D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Development+of+a+murine+model+of+chronic+Salmonella+infection.">Development of a murine model of chronic Salmonella infection.</a> Infection and immunity. 65 (2), 838-842 (1997).</li><li>. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> Biosafety in Microbiological and Biomedical Laboratories (BMBL). , (2007).</li><li>Wu, S., Lu, R., Zhang, Y., Sun, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Chronic+effects+of+a+Salmonella+type+III+secretion+effector+protein+AvrA+in+vivo.">Chronic effects of a Salmonella type III secretion effector protein AvrA in vivo.</a> PLoS ONE. , (2010).</li></ol>

Per utilizzare questo sistema, è necessario che il ricercatore per imparare a sonda gastrica degli animali. Noi dettaglio una metodologia per preparare la cultura batterica e sonda gastrica i topi. Mostriamo anche come monitorare la persistenza Salmonella nel tratto gastrointestinale (GI). Le fasi critiche in questo protocollo, tra cui: <ol><li> Streptomicina-pretrattamento: Streptomicina-pretrattamento riusciva a liberarsi di qualche commensale della flora intestinale e fanno i topi suscettibili alla infe...

<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" "http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd">
<html xmlns="http://www.w3.org/1999/xhtml" xml:lang="en" lang="en">	
		
		<div>
		<table border="1">
		<thead>
		<tr>
		<th>Material Name</th>
		<th>Type</th>
		<th>Company</th>
		<th>Catalogue Number</th>
		<th>Comment</th>
		</tr>
		</thead>
		<tbody>
		
<tr>
<td>HBSS</td>
<td>&nbsp;</td>
<td>Sigma</td>
<td>ABCD1234</td>
<td>&nbsp;</td>
<tr>
<td>Luria-Bertani broth</td>
<td>&nbsp;</td>
<td>BD</td>
<td>244610</td>
<td>&nbsp;</td>
<tr>
<td>Feeding needle</td>
<td>&nbsp;</td>
<td>Popper &amp; Sons, Inc</td>
<td>7920</td>
<td>&nbsp;</td>
<tr>
<td>Streptomycin </td>
<td>&nbsp;</td>
<td>MP biomedicals, LLC</td>
<td>100556</td>
<td>&nbsp;</td>
<tr>
<td>BBL CHROMagar Salmonella</td>
<td>&nbsp;</td>
<td>BD</td>
<td>214983</td>
<td>&nbsp;</td>
<tr>
<td>Disposable cell spreaders</td>
<td>&nbsp;</td>
<td>Biologix research company</td>
<td>65-1010</td>
<td>&nbsp;</td>		</tbody>
		</table>
		</div>

chronic salmonella infected mouse model

Il modello di infezione batterica del mouse è un sistema potente modello per lo studio di aree come infezioni, infiammazioni, immunologia, trasduzione del segnale, e tumorigenesi. Molti ricercatori hanno approfittato della colite indotta da<em&gt; Salmonella</emTyphimurium&gt; per gli studi sulla fase precoce di infiammazione e infezione. Tuttavia, solo pochi studi sono l&#39;infezione cronica<em&gt; In vivo</em&gt;. I topi con<em&gt; Salmonella</emEsistenza&gt; persistente nel tratto gastrointestinale ci permettono di esplorare a lungo termine di interazione ospite-batterico, trasduzione del segnale, e tumorigenesi. Abbiamo stabilito un modello batterica cronica del mouse infettati con<em&gt; Salmonella</em&gt; Colonizzazione typhimurium nell&#39;intestino del mouse di oltre 6 mesi. Per utilizzare questo sistema, è necessario che il ricercatore per imparare a preparare la coltura batterica e sonda gastrica degli animali. Noi dettaglio una metodologia per la preparazione coltura batterica e topi sonda gastrica. Mostriamo anche come rilevare la<em&gt; Salmonella</emPersistenza&gt; nel tratto gastrointestinale. Nel complesso, questo protocollo sarà di aiuto ricercatori che utilizzano il modello di infezione batterica del mouse per affrontare le questioni biologiche e microbiologiche di fondamentale importanza.

Watch this Scientific Journal Video about Chronic Salmonella Infected Mouse Model at JoVE.com

Chronic Salmonella Infected Mouse Model

Stabilire un modello batterica cronica del mouse infettati con persistente<em&gt; Salmonella</em&gt; Colonizzazione typhimurium nell&#39;intestino per 27 settimane.

Cronico Salmonella Modello mouse infetti

The bacterial infected mouse model is a powerful model system for studying areas such as infection, inflammation, immunology, signal transduction, and ...

Research

JoVE Journal

Biology

Chronic Salmonella Infected Mouse Model

Orthotopic Mouse Model of Colorectal Cancer

Topo Modello ortotopico di cancro colorettale

The traditional subcutaneous tumor model is less than ideal for studying colorectal cancer. Orthotopic mouse models of colorectal cancer, which feature ...

Ricerca

Biologia

Laser-Induced Chronic Ocular Hypertension Model on SD Rats

Laser-Induced cronica ipertensione oculare su Ratti modello SD

Glaucoma is one of the major causes of blindness in the world. Elevated intraocular pressure is a major risk factor. Laser photocoagulation induced ocular ...

Immunohistochemistry on Paraffin Sections of Mouse Epidermis Using Fluorescent Antibodies

Immunoistochimica su sezioni di paraffina di epidermide mouse utilizzando anticorpi fluorescenti

In the epidermis, immunohistochemistry is an efficient means of localizing specific proteins to their relative expression compartment; namely the basal, ...

In vivo Imaging and Therapeutic Treatments in an Orthotopic Mouse Model of Ovarian Cancer

In vivo Imaging and Therapeutic Treatments in an Orthotopic Mouse Model of Ovarian Cancer

Imaging in vivo e trattamenti terapeutici in un modello di mouse ortotopico di cancro ovarico

Human cancer and response to therapy is better represented in orthotopic animal models.  This paper describes the development of an orthotopic mouse model ...

Generation of Mice Derived from Induced Pluripotent Stem Cells

Generazione di topi derivati ​​da cellule staminali pluripotenti indotte

The production of induced pluripotent stem cells (iPSCs) from somatic cells provides a means to create valuable tools for basic research and may also ...

Maintaining Aedes aegypti Mosquitoes Infected with Wolbachia

Maintaining Aedes aegypti Mosquitoes Infected with Wolbachia

Mantenimento di Aedes aegypti zanzare infettate con Wolbachia

Aedes aegypti mosquitoes experimentally infected with Wolbachia are being utilized in programs to control the spread of arboviruses such as dengue, ...

The Murine Choline-Deficient, Ethionine-Supplemented (CDE) Diet Model of Chronic Liver Injury

The Murine Choline-Deficient, Ethionine-Supplemented CDE Diet Model of Chronic Liver Injury

La murino colina-carenti, etionina-completati (CDE) dieta modello della lesione epatica cronica

Chronic liver diseases, such as viral hepatitis, alcoholic liver disease, or non-alcoholic fatty liver disease, are characterized by continual ...

Trans-inner Cell Mass Injection of Embryonic Stem Cells Leads to Higher Chimerism Rates

Trans-inner Cell Mass iniezione delle cellule staminali embrionali porta a tassi più elevati di chimerismo

In an effort to increase efficiency in the creation of genetically modified mice via ES Cell methodologies, we present an adaptation to the current ...

A Mouse Model of Intestinal Partial Obstruction

Un modello murino di ostruzione intestinale parziale

Intestinal obstructions, that impede or block peristaltic movement, can be caused by abdominal adhesions and most gastrointestinal (GI) diseases including ...

Quasi-metagenomic Analysis of Salmonella from Food and Environmental Samples

Quasi-metagenomic Analysis of Salmonella from Food and Environmental Samples

Analisi metagenomica quasi di Salmonella da campioni ambientali e alimentari

Quasi-metagenomics sequencing refers to the sequencing-based analysis of modified microbiomes of food and environmental samples. In this protocol, ...