Name: chronic salmonella infected mouse model
Uploaded: 2010-05-31T17:00:00
Description: Watch this Scientific Journal Video about Chronic Salmonella Infected Mouse Model at JoVE.com

Detta arbete stöddes av NIDDK KO1 DK075386 bidrag och American Cancer Society RSG-09-075-01-MBC till juni Sön

Detta protokoll innehåller tre delar: bakteriekultur, mus sondmatning, och Salmonella upptäckt. 1. Salmonella tillväxt skick. <ol><li> Förbered Salmonella Luria-Bertani buljong (LB) agar, inkubera vid 37 ° C över natten. </li><li> Välj en klon från LB plattan och tas i 7 ml LB i en 12 ml tub, och skaka vid 37 ° C i ca 5 timmar. </li><li> Inokulera 50 ml LB med 0,05 ml av en stationär fas kultur och inkubera vid 37 ° C utan att skaka i ca 18 timmar. </l...

<ol>
	<li>Duan, Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=beta-Catenin+activity+negatively+regulates+bacteria-induced+inflammation.">beta-Catenin activity negatively regulates bacteria-induced inflammation.</a> Lab Invest. 87 (6), 613-624 (2007).</li><li>Barthel, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Pretreatment+of+mice+with+streptomycin+provides+a+Salmonella+enterica+serovar+Typhimurium+colitis+model+that+allows+analysis+of+both+pathogen+and+host.">Pretreatment of mice with streptomycin provides a Salmonella enterica serovar Typhimurium colitis model that allows analysis of both pathogen and host.</a> Infection and immunity. 71 (5), 2839-2858 (2003).</li><li>Miller, S. I., Mekalanos, J. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Constitutive+expression+of+the+phoP+regulon+attenuates+Salmonella+virulence+and+survival+within+macrophages.">Constitutive expression of the phoP regulon attenuates Salmonella virulence and survival within macrophages.</a> Journal of bacteriology. 172 (5), 2485-2490 (1990).</li><li>Valdez, Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Nramp1+expression+by+dendritic+cells+modulates+inflammatory+responses+during+Salmonella+Typhimurium+infection.">Nramp1 expression by dendritic cells modulates inflammatory responses during Salmonella Typhimurium infection.</a> Cell Microbiol. 10 (8), 1646-1661 (2008).</li><li>Woo, H., Okamoto, S., Guiney, D., Gunn, J. S., Fierer, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+model+of+Salmonella+colitis+with+features+of+diarrhea+in+SLC11A1+wild-type+mice.">A model of Salmonella colitis with features of diarrhea in SLC11A1 wild-type mice.</a> PLoS ONE. 3 (2), e1603-e1603 (2008).</li><li>Dangl, J. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Molecular+call-and-response:+how+Salmonella+learns+the+gospel+from+its+host.">Molecular call-and-response: how Salmonella learns the gospel from its host.</a> Trends Microbiol. 11 (6), 245-246 (2003).</li><li>Zaharik, M. L., Vallance, B. A., Puente, J. L., Gros, P., Finlay, B. 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L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Humane+endpoints+for+infectious+disease+animal+models.">Humane endpoints for infectious disease animal models.</a> ILAR J. 41 (2), 99-104 (2000).</li><li>Ye, Z., Petrof, E. O., Boone, D., Claud, E. C., Sun, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Salmonella+effector+AvrA+regulation+of+colonic+epithelial+cell+inflammation+by+deubiquitination.">Salmonella effector AvrA regulation of colonic epithelial cell inflammation by deubiquitination.</a> Am J Pathol. 171 (3), 882-892 (2007).</li><li>Grassl, G. A., Valdez, Y., Bergstrom, K. S., Vallance, B. A., Finlay, B. B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Chronic+enteric+salmonella+infection+in+mice+leads+to+severe+and+persiste+nt+intestinal+fibrosis.">Chronic enteric salmonella infection in mice leads to severe and persiste nt intestinal fibrosis.</a> Gastroenterology. 134 (3), 768-780 (2008).</li><li>Sukupolvi, S., Edelstein, A., Rhen, M., Normark, S. J., Pfeifer, J. 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För att använda detta system är det nödvändigt för forskaren att lära sig att sondmatning djuren. Vi detalj en metod för att förbereda bakteriekultur och sondmatning till möss. Vi visar också hur man kan övervaka Salmonella persistens i gastrointestinal (GI) tarmkanalen. Den kritiska steg i detta protokoll, inklusive: <ol><li> Streptomycin-förbehandling: streptomycin-förbehandling kan bli av med några kommensala tarmflora och göra möss mottagliga för salmonella infektion 2.</...

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		<th>Material Name</th>
		<th>Type</th>
		<th>Company</th>
		<th>Catalogue Number</th>
		<th>Comment</th>
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<td>HBSS</td>
<td>&nbsp;</td>
<td>Sigma</td>
<td>ABCD1234</td>
<td>&nbsp;</td>
<tr>
<td>Luria-Bertani broth</td>
<td>&nbsp;</td>
<td>BD</td>
<td>244610</td>
<td>&nbsp;</td>
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<td>Feeding needle</td>
<td>&nbsp;</td>
<td>Popper &amp; Sons, Inc</td>
<td>7920</td>
<td>&nbsp;</td>
<tr>
<td>Streptomycin </td>
<td>&nbsp;</td>
<td>MP biomedicals, LLC</td>
<td>100556</td>
<td>&nbsp;</td>
<tr>
<td>BBL CHROMagar Salmonella</td>
<td>&nbsp;</td>
<td>BD</td>
<td>214983</td>
<td>&nbsp;</td>
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<td>Disposable cell spreaders</td>
<td>&nbsp;</td>
<td>Biologix research company</td>
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chronic salmonella infected mouse model

Den bakterie infekterade musmodell är ett kraftfullt modellsystem för att studera områden som infektion, inflammation, immunologi, signaltransduktion, och Tumörgenesens. Många forskare har dragit nytta av kolit inducerad av<em&gt; Salmonella</em&gt; Typhimurium för studier av den tidiga fasen av inflammation och infektion. Men bara ett fåtal rapporter på kronisk infektion<em&gt; In vivo</em&gt;. Möss med<em&gt; Salmonella</em&gt; Ihållande existens i mag-tarmkanalen ger oss möjlighet att utforska den långsiktiga host-bakteriell interaktion, signaltransduktion, och Tumörgenesens. Vi har etablerat en kronisk bakteriell infekterad musmodell med<em&gt; Salmonella</em&gt; Typhimurium kolonisationen i musen tarmen över 6 månader. För att använda detta system är det nödvändigt för forskaren att lära sig att förbereda bakterieodling och sondmatning djuren. Vi detalj en metod för att förbereda bakteriekultur och sondmatning möss. Vi visar också hur man kan upptäcka<em&gt; Salmonella</em&gt; Persistens i mag-tarmkanalen. Sammantaget kommer detta protokoll bistånd forskare som använder den bakteriella smittade musmodell för att ta itu principiellt viktiga biologiska och mikrobiologiska frågor.

Watch this Scientific Journal Video about Chronic Salmonella Infected Mouse Model at JoVE.com

Chronic Salmonella Infected Mouse Model

Upprätta en kronisk bakteriell infekterad musmodell med ihållande<em&gt; Salmonella</em&gt; Typhimurium kolonisering i tarmen ytterligare 27 veckor.

Kronisk Salmonella Infected musmodell

The bacterial infected mouse model is a powerful model system for studying areas such as infection, inflammation, immunology, signal transduction, and ...

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Biology

Chronic Salmonella Infected Mouse Model

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