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DOI :
10.3791/1948-v
•
9:16 min
June 15th, 2010
Chapters
0:45
Introduction
1:15
Preparation of Sucrose Gradients
2:53
Preparation of Extracts from Yeast Cells
8:35
Representative Trace of a Ribosome Extract
5:06
Centrifugation of Gradients and Collection of Data and Fractions
8:56
Conclusion
本文介绍了协议的真核细胞的核糖体翻译的提取。一旦提取,核糖体是由蔗糖梯度分馏分离到monosomes和多聚核糖体,允许不同的核糖体的人群进行分析。因此,这种方法是研究翻译调控的黄金标准。
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