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DOI :
10.3791/1948-v
•
9:16 min
June 15th, 2010
Chapters
0:45
Introduction
1:15
Preparation of Sucrose Gradients
2:53
Preparation of Extracts from Yeast Cells
8:35
Representative Trace of a Ribosome Extract
5:06
Centrifugation of Gradients and Collection of Data and Fractions
8:56
Conclusion
이 문서는 진핵 세포의 변이를 번역의 추출을위한 프로토콜을 설명합니다. 일단 추출된, 리보솜은 다른 ribosomal 인구가 분석 수 있도록 자당 기울기 분별 (분리)에 의해 monosomes 및 polyribosomes로 구분됩니다. 따라서이 방법은 번역의 규정을 조사를위한 황금 표준입니다.
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