Name: flash freezing and cryosectioning e12.5 mouse brain
Uploaded: 2007-05-28T00:00:00
Description: Watch this Scientific Journal Video about Flash Freezing and Cryosectioning E12.5 Mouse Brain at JoVE.com

<ol><li> Fixer le tissu dans du paraformaldéhyde 4% dans du PBS pendant le temps désiré. </li><li> Saccharose infuser tissus (cryoprotection) <ol><li> Faire solution de saccharose à 30% dans le PBS p / v en 2059 tube. </li><li> Rincez le tissu 3x dans du PBS (~ 5 min avec bascule). </li><li> Tissulaire lieu en solution de saccharose à 30%. Tissus ne va pas couler. </li><li> Placez le tissu dans 4 ° C pendant la nuit, ou jusqu&#39;à ce qu&#39;il ait coulé. </li></ol></li><li> Taille de l&#39;étiquette appropriée cryomold d&#39;informat...

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		<th>Material Name</th>
		<th>Type</th>
		<th>Company</th>
		<th>Catalogue Number</th>
		<th>Comment</th>
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<td>Tissue-Tek Cryomold</td>
<td>&nbsp;</td>
<td>Ted Pella, Inc.</td>
<td>27181</td>
<td>&nbsp;</td>
<tr>
<td>O.C.T.</td>
<td>&nbsp;</td>
<td>Ted Pella, Inc</td>
<td>27050</td>
<td>&nbsp;</td>
<tr>
<td>Sucrose solution</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>30% sucrose solution in PBS w/v</td>
</tr>
<tr>
<td>paraformaldehyde</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>4% paraformaldehyde in PBS </td>
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flash freezing and cryosectioning e12.5 mouse brain

Watch this Scientific Journal Video about Flash Freezing and Cryosectioning E12.5 Mouse Brain at JoVE.com

Flash Freezing and Cryosectioning E12.5 Mouse Brain

<p class="jove_content">Démonstration dans cette vidéo sont les techniques pour flash de congélation et de sectionnement des tissus cérébraux embryonnaires à partir de la souris. Conseils utiles pour l&#39;utilisation du cryostat sont donnés, y compris les méthodes de dépannage qui peut être utilisé pendant la coupe pour s&#39;assurer que les sections résultante tissus sont exempts de fissures et d&#39;autres distorsions.</p>

Flash de congélation et Cryosectioning E12.5 cerveau de souris

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