Name: flash freezing and cryosectioning e12.5 mouse brain
Uploaded: 2007-05-28T00:00:00
Description: Watch this Scientific Journal Video about Flash Freezing and Cryosectioning E12.5 Mouse Brain at JoVE.com

<ol style=";text-align:right;direction:rtl"><li style=";text-align:right;direction:rtl"> תיקון רקמות paraformaldehyde 4% PBS בפעם הרצוי. </li><li style=";text-align:right;direction:rtl"> להשרות סוכרוז רקמות (cryoprotection) <ol style=";text-align:right;direction:rtl"><li style=";text-align:right;direction:rtl"> בצע פתרון סוכרוז 30% PBS w / v בצינור 2059. </li><li style=";text-align:right;direction:rtl"> יש לשטוף את רקמת 3x ב PBS (~ 5 דקות עם נדנדה). </li><li style=...

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		<thead>
		<tr>
		<th>Material Name</th>
		<th>Type</th>
		<th>Company</th>
		<th>Catalogue Number</th>
		<th>Comment</th>
		</tr>
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		<tbody>
		
<tr>
<td>Tissue-Tek Cryomold</td>
<td>&nbsp;</td>
<td>Ted Pella, Inc.</td>
<td>27181</td>
<td>&nbsp;</td>
<tr>
<td>O.C.T.</td>
<td>&nbsp;</td>
<td>Ted Pella, Inc</td>
<td>27050</td>
<td>&nbsp;</td>
<tr>
<td>Sucrose solution</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>30% sucrose solution in PBS w/v</td>
</tr>
<tr>
<td>paraformaldehyde</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>4% paraformaldehyde in PBS </td>
</tr>		</tbody>
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flash freezing and cryosectioning e12.5 mouse brain

Watch this Scientific Journal Video about Flash Freezing and Cryosectioning E12.5 Mouse Brain at JoVE.com

Flash Freezing and Cryosectioning E12.5 Mouse Brain

<p class="jove_content">הפגינו וידאו זו הן טכניקות פלאש מקפיא חתך ברקמת המוח העובריים של העכבר. טיפים שימושיים לשימוש cryostat מקבלים, כולל שיטות לפתרון בעיות שניתן להשתמש בהם בעת חיתוך על מנת להבטיח כי הסעיפים רקמות כתוצאה חופשיים של סדקים ועיוותים אחרים.</p>

פלאש מקפיא Cryosectioning E12.5 מוח עכבר

Research

JoVE Journal

Biology

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The colonic mucosal tissue provides a vital barrier to luminal antigens. This barrier is composed of a monolayer of simple columnar epithelial cells. The ...

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